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Poly-dA:dT Tracts Form an In Vivo Nucleosomal Turnstile
Nucleosomes regulate many DNA-dependent processes by controlling the accessibility of DNA, and DNA sequences such as the poly-dA:dT element are known to affect nucleosome binding. We demonstrate that poly-dA:dT tracts form an asymmetric barrier to nucleosome movement in vivo, mediated by ATP-depende...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212969/ https://www.ncbi.nlm.nih.gov/pubmed/25353956 http://dx.doi.org/10.1371/journal.pone.0110479 |
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author | de Boer, Carl G. Hughes, Timothy R. |
author_facet | de Boer, Carl G. Hughes, Timothy R. |
author_sort | de Boer, Carl G. |
collection | PubMed |
description | Nucleosomes regulate many DNA-dependent processes by controlling the accessibility of DNA, and DNA sequences such as the poly-dA:dT element are known to affect nucleosome binding. We demonstrate that poly-dA:dT tracts form an asymmetric barrier to nucleosome movement in vivo, mediated by ATP-dependent chromatin remodelers. We theorize that nucleosome transit over poly-A elements is more energetically favourable in one direction, leading to an asymmetric arrangement of nucleosomes around these sequences. We demonstrate that different arrangements of poly-A and poly-T tracts result in very different outcomes for nucleosome occupancy in yeast, mouse, and human, and show that yeast takes advantage of this phenomenon in its promoter architecture. |
format | Online Article Text |
id | pubmed-4212969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42129692014-11-05 Poly-dA:dT Tracts Form an In Vivo Nucleosomal Turnstile de Boer, Carl G. Hughes, Timothy R. PLoS One Research Article Nucleosomes regulate many DNA-dependent processes by controlling the accessibility of DNA, and DNA sequences such as the poly-dA:dT element are known to affect nucleosome binding. We demonstrate that poly-dA:dT tracts form an asymmetric barrier to nucleosome movement in vivo, mediated by ATP-dependent chromatin remodelers. We theorize that nucleosome transit over poly-A elements is more energetically favourable in one direction, leading to an asymmetric arrangement of nucleosomes around these sequences. We demonstrate that different arrangements of poly-A and poly-T tracts result in very different outcomes for nucleosome occupancy in yeast, mouse, and human, and show that yeast takes advantage of this phenomenon in its promoter architecture. Public Library of Science 2014-10-29 /pmc/articles/PMC4212969/ /pubmed/25353956 http://dx.doi.org/10.1371/journal.pone.0110479 Text en © 2014 de Boer, Hughes http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article de Boer, Carl G. Hughes, Timothy R. Poly-dA:dT Tracts Form an In Vivo Nucleosomal Turnstile |
title | Poly-dA:dT Tracts Form an In Vivo Nucleosomal Turnstile |
title_full | Poly-dA:dT Tracts Form an In Vivo Nucleosomal Turnstile |
title_fullStr | Poly-dA:dT Tracts Form an In Vivo Nucleosomal Turnstile |
title_full_unstemmed | Poly-dA:dT Tracts Form an In Vivo Nucleosomal Turnstile |
title_short | Poly-dA:dT Tracts Form an In Vivo Nucleosomal Turnstile |
title_sort | poly-da:dt tracts form an in vivo nucleosomal turnstile |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4212969/ https://www.ncbi.nlm.nih.gov/pubmed/25353956 http://dx.doi.org/10.1371/journal.pone.0110479 |
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