Identification of DNA Aptamers toward Epithelial Cell Adhesion Molecule via Cell-SELEX

The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate t...

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Detalles Bibliográficos
Autores principales: Kim, Ji Won, Kim, Eun Young, Kim, Sun Young, Byun, Sang Kyung, Lee, Dasom, Oh, Kyoung-Jin, Kim, Won Kon, Han, Baek Soo, Chi, Seung-Wook, Lee, Sang Chul, Bae, Kwang-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4213765/
https://www.ncbi.nlm.nih.gov/pubmed/25266702
http://dx.doi.org/10.14348/molcells.2014.0208
Descripción
Sumario:The epithelial cell adhesion molecule (EpCAM, also known as CD326) is a transmembrane glycoprotein that is specifically detected in most adenocarcinomas and cancer stem cells. In this study, we performed a Cell systematic evolution of ligands by exponential enrichment (SELEX) experiment to isolate the aptamers against EpCAM. After seven round of Cell SELEX, we identified several aptamer candidates. Among the selected aptamers, EP166 specifically binds to cells expressing EpCAM with an equilibrium dissociation constant (Kd) in a micromolar range. On the other hand, it did not bind to negative control cells. Moreover, EP166 binds to J1ES cells, a mouse embryonic stem cell line. Therefore, the isolated aptamers against EpCAM could be used as a stem cell marker or in other applications in both stem cell and cancer studies.

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