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TACC3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types

Microtubule plus end dynamics are regulated by a conserved family of proteins called plus end–tracking proteins (+TIPs). It is unclear how various +TIPs interact with each other and with plus ends to control microtubule behavior. The centrosome-associated protein TACC3, a member of the transforming...

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Autores principales: Nwagbara, Belinda U., Faris, Anna E., Bearce, Elizabeth A., Erdogan, Burcu, Ebbert, Patrick T., Evans, Matthew F., Rutherford, Erin L., Enzenbacher, Tiffany B., Lowery, Laura Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214782/
https://www.ncbi.nlm.nih.gov/pubmed/25187649
http://dx.doi.org/10.1091/mbc.E14-06-1121
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author Nwagbara, Belinda U.
Faris, Anna E.
Bearce, Elizabeth A.
Erdogan, Burcu
Ebbert, Patrick T.
Evans, Matthew F.
Rutherford, Erin L.
Enzenbacher, Tiffany B.
Lowery, Laura Anne
author_facet Nwagbara, Belinda U.
Faris, Anna E.
Bearce, Elizabeth A.
Erdogan, Burcu
Ebbert, Patrick T.
Evans, Matthew F.
Rutherford, Erin L.
Enzenbacher, Tiffany B.
Lowery, Laura Anne
author_sort Nwagbara, Belinda U.
collection PubMed
description Microtubule plus end dynamics are regulated by a conserved family of proteins called plus end–tracking proteins (+TIPs). It is unclear how various +TIPs interact with each other and with plus ends to control microtubule behavior. The centrosome-associated protein TACC3, a member of the transforming acidic coiled-coil (TACC) domain family, has been implicated in regulating several aspects of microtubule dynamics. However, TACC3 has not been shown to function as a +TIP in vertebrates. Here we show that TACC3 promotes axon outgrowth and regulates microtubule dynamics by increasing microtubule plus end velocities in vivo. We also demonstrate that TACC3 acts as a +TIP in multiple embryonic cell types and that this requires the conserved C-terminal TACC domain. Using high-resolution live-imaging data on tagged +TIPs, we show that TACC3 localizes to the extreme microtubule plus end, where it lies distal to the microtubule polymerization marker EB1 and directly overlaps with the microtubule polymerase XMAP215. TACC3 also plays a role in regulating XMAP215 stability and localizing XMAP215 to microtubule plus ends. Taken together, our results implicate TACC3 as a +TIP that functions with XMAP215 to regulate microtubule plus end dynamics.
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spelling pubmed-42147822015-01-16 TACC3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types Nwagbara, Belinda U. Faris, Anna E. Bearce, Elizabeth A. Erdogan, Burcu Ebbert, Patrick T. Evans, Matthew F. Rutherford, Erin L. Enzenbacher, Tiffany B. Lowery, Laura Anne Mol Biol Cell Articles Microtubule plus end dynamics are regulated by a conserved family of proteins called plus end–tracking proteins (+TIPs). It is unclear how various +TIPs interact with each other and with plus ends to control microtubule behavior. The centrosome-associated protein TACC3, a member of the transforming acidic coiled-coil (TACC) domain family, has been implicated in regulating several aspects of microtubule dynamics. However, TACC3 has not been shown to function as a +TIP in vertebrates. Here we show that TACC3 promotes axon outgrowth and regulates microtubule dynamics by increasing microtubule plus end velocities in vivo. We also demonstrate that TACC3 acts as a +TIP in multiple embryonic cell types and that this requires the conserved C-terminal TACC domain. Using high-resolution live-imaging data on tagged +TIPs, we show that TACC3 localizes to the extreme microtubule plus end, where it lies distal to the microtubule polymerization marker EB1 and directly overlaps with the microtubule polymerase XMAP215. TACC3 also plays a role in regulating XMAP215 stability and localizing XMAP215 to microtubule plus ends. Taken together, our results implicate TACC3 as a +TIP that functions with XMAP215 to regulate microtubule plus end dynamics. The American Society for Cell Biology 2014-11-01 /pmc/articles/PMC4214782/ /pubmed/25187649 http://dx.doi.org/10.1091/mbc.E14-06-1121 Text en © 2014 Nwagbara, Faris, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Nwagbara, Belinda U.
Faris, Anna E.
Bearce, Elizabeth A.
Erdogan, Burcu
Ebbert, Patrick T.
Evans, Matthew F.
Rutherford, Erin L.
Enzenbacher, Tiffany B.
Lowery, Laura Anne
TACC3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types
title TACC3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types
title_full TACC3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types
title_fullStr TACC3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types
title_full_unstemmed TACC3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types
title_short TACC3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types
title_sort tacc3 is a microtubule plus end–tracking protein that promotes axon elongation and also regulates microtubule plus end dynamics in multiple embryonic cell types
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214782/
https://www.ncbi.nlm.nih.gov/pubmed/25187649
http://dx.doi.org/10.1091/mbc.E14-06-1121
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