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Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature

Phosphatidylinositol-4-phosphate (PI4P) is produced on both the Golgi and the plasma membrane. Despite extensive vesicular traffic between these compartments, genetic analysis suggests that the two pools of PI4P do not efficiently mix with one another. Several lines of evidence indicate that the PI4...

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Detalles Bibliográficos
Autores principales: Ling, Yading, Hayano, Scott, Novick, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214785/
https://www.ncbi.nlm.nih.gov/pubmed/25165144
http://dx.doi.org/10.1091/mbc.E14-06-1087
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author Ling, Yading
Hayano, Scott
Novick, Peter
author_facet Ling, Yading
Hayano, Scott
Novick, Peter
author_sort Ling, Yading
collection PubMed
description Phosphatidylinositol-4-phosphate (PI4P) is produced on both the Golgi and the plasma membrane. Despite extensive vesicular traffic between these compartments, genetic analysis suggests that the two pools of PI4P do not efficiently mix with one another. Several lines of evidence indicate that the PI4P produced on the Golgi is normally incorporated into secretory vesicles, but the fate of that pool has been unclear. We show here that in yeast the oxysterol-binding proteins Osh1–Osh7 are collectively needed to maintain the normal distribution of PI4P and that Osh4p is critical in this function. Osh4p associates with secretory vesicles at least in part through its interaction with PI4P and is needed, together with lipid phosphatases, to reduce the level of PI4P as vesicles approach sites of exocytosis. This reduction in PI4P is necessary for a switch in the regulation of the Sec4p exchange protein, Sec2p, from an interaction with the upstream Rab, Ypt31/32, to an interaction with a downstream Sec4p effector, Sec15p. Spatial regulation of PI4P levels thereby plays an important role in vesicle maturation.
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spelling pubmed-42147852015-01-16 Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature Ling, Yading Hayano, Scott Novick, Peter Mol Biol Cell Articles Phosphatidylinositol-4-phosphate (PI4P) is produced on both the Golgi and the plasma membrane. Despite extensive vesicular traffic between these compartments, genetic analysis suggests that the two pools of PI4P do not efficiently mix with one another. Several lines of evidence indicate that the PI4P produced on the Golgi is normally incorporated into secretory vesicles, but the fate of that pool has been unclear. We show here that in yeast the oxysterol-binding proteins Osh1–Osh7 are collectively needed to maintain the normal distribution of PI4P and that Osh4p is critical in this function. Osh4p associates with secretory vesicles at least in part through its interaction with PI4P and is needed, together with lipid phosphatases, to reduce the level of PI4P as vesicles approach sites of exocytosis. This reduction in PI4P is necessary for a switch in the regulation of the Sec4p exchange protein, Sec2p, from an interaction with the upstream Rab, Ypt31/32, to an interaction with a downstream Sec4p effector, Sec15p. Spatial regulation of PI4P levels thereby plays an important role in vesicle maturation. The American Society for Cell Biology 2014-11-01 /pmc/articles/PMC4214785/ /pubmed/25165144 http://dx.doi.org/10.1091/mbc.E14-06-1087 Text en © 2014 Ling et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Ling, Yading
Hayano, Scott
Novick, Peter
Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature
title Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature
title_full Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature
title_fullStr Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature
title_full_unstemmed Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature
title_short Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature
title_sort osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214785/
https://www.ncbi.nlm.nih.gov/pubmed/25165144
http://dx.doi.org/10.1091/mbc.E14-06-1087
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