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Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature
Phosphatidylinositol-4-phosphate (PI4P) is produced on both the Golgi and the plasma membrane. Despite extensive vesicular traffic between these compartments, genetic analysis suggests that the two pools of PI4P do not efficiently mix with one another. Several lines of evidence indicate that the PI4...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214785/ https://www.ncbi.nlm.nih.gov/pubmed/25165144 http://dx.doi.org/10.1091/mbc.E14-06-1087 |
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author | Ling, Yading Hayano, Scott Novick, Peter |
author_facet | Ling, Yading Hayano, Scott Novick, Peter |
author_sort | Ling, Yading |
collection | PubMed |
description | Phosphatidylinositol-4-phosphate (PI4P) is produced on both the Golgi and the plasma membrane. Despite extensive vesicular traffic between these compartments, genetic analysis suggests that the two pools of PI4P do not efficiently mix with one another. Several lines of evidence indicate that the PI4P produced on the Golgi is normally incorporated into secretory vesicles, but the fate of that pool has been unclear. We show here that in yeast the oxysterol-binding proteins Osh1–Osh7 are collectively needed to maintain the normal distribution of PI4P and that Osh4p is critical in this function. Osh4p associates with secretory vesicles at least in part through its interaction with PI4P and is needed, together with lipid phosphatases, to reduce the level of PI4P as vesicles approach sites of exocytosis. This reduction in PI4P is necessary for a switch in the regulation of the Sec4p exchange protein, Sec2p, from an interaction with the upstream Rab, Ypt31/32, to an interaction with a downstream Sec4p effector, Sec15p. Spatial regulation of PI4P levels thereby plays an important role in vesicle maturation. |
format | Online Article Text |
id | pubmed-4214785 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-42147852015-01-16 Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature Ling, Yading Hayano, Scott Novick, Peter Mol Biol Cell Articles Phosphatidylinositol-4-phosphate (PI4P) is produced on both the Golgi and the plasma membrane. Despite extensive vesicular traffic between these compartments, genetic analysis suggests that the two pools of PI4P do not efficiently mix with one another. Several lines of evidence indicate that the PI4P produced on the Golgi is normally incorporated into secretory vesicles, but the fate of that pool has been unclear. We show here that in yeast the oxysterol-binding proteins Osh1–Osh7 are collectively needed to maintain the normal distribution of PI4P and that Osh4p is critical in this function. Osh4p associates with secretory vesicles at least in part through its interaction with PI4P and is needed, together with lipid phosphatases, to reduce the level of PI4P as vesicles approach sites of exocytosis. This reduction in PI4P is necessary for a switch in the regulation of the Sec4p exchange protein, Sec2p, from an interaction with the upstream Rab, Ypt31/32, to an interaction with a downstream Sec4p effector, Sec15p. Spatial regulation of PI4P levels thereby plays an important role in vesicle maturation. The American Society for Cell Biology 2014-11-01 /pmc/articles/PMC4214785/ /pubmed/25165144 http://dx.doi.org/10.1091/mbc.E14-06-1087 Text en © 2014 Ling et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Ling, Yading Hayano, Scott Novick, Peter Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature |
title | Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature |
title_full | Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature |
title_fullStr | Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature |
title_full_unstemmed | Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature |
title_short | Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature |
title_sort | osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4214785/ https://www.ncbi.nlm.nih.gov/pubmed/25165144 http://dx.doi.org/10.1091/mbc.E14-06-1087 |
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