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Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation

BACKGROUND: Carbon nanotubes (CNT) represent a great promise for technological and industrial development but serious concerns on their health effects have also emerged. Rod-shaped CNT are, in fact, able to induce asbestos-like pathogenicity in mice including granuloma formation in abdominal cavity...

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Autores principales: Rydman, Elina M, Ilves, Marit, Koivisto, Antti J, Kinaret, Pia A S, Fortino, Vittorio, Savinko, Terhi S, Lehto, Maili T, Pulkkinen, Ville, Vippola, Minnamari, Hämeri, Kaarle J, Matikainen, Sampsa, Wolff, Henrik, Savolainen, Kai M, Greco, Dario, Alenius, Harri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215016/
https://www.ncbi.nlm.nih.gov/pubmed/25318534
http://dx.doi.org/10.1186/s12989-014-0048-2
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author Rydman, Elina M
Ilves, Marit
Koivisto, Antti J
Kinaret, Pia A S
Fortino, Vittorio
Savinko, Terhi S
Lehto, Maili T
Pulkkinen, Ville
Vippola, Minnamari
Hämeri, Kaarle J
Matikainen, Sampsa
Wolff, Henrik
Savolainen, Kai M
Greco, Dario
Alenius, Harri
author_facet Rydman, Elina M
Ilves, Marit
Koivisto, Antti J
Kinaret, Pia A S
Fortino, Vittorio
Savinko, Terhi S
Lehto, Maili T
Pulkkinen, Ville
Vippola, Minnamari
Hämeri, Kaarle J
Matikainen, Sampsa
Wolff, Henrik
Savolainen, Kai M
Greco, Dario
Alenius, Harri
author_sort Rydman, Elina M
collection PubMed
description BACKGROUND: Carbon nanotubes (CNT) represent a great promise for technological and industrial development but serious concerns on their health effects have also emerged. Rod-shaped CNT are, in fact, able to induce asbestos-like pathogenicity in mice including granuloma formation in abdominal cavity and sub-pleural fibrosis. Exposure to CNT, especially in the occupational context, happens mainly by inhalation. However, little is known about the possible effects of CNT on pulmonary allergic diseases, such as asthma. METHODS: We exposed mice by inhalation to two types of multi-walled CNT, rigid rod-like and flexible tangled CNT, for four hours a day once or on four consecutive days. Early events were monitored immediately and 24 hours after the single inhalation exposure and the four day exposure mimicked an occupational work week. Mast cell deficient mice were used to evaluate the role of mast cells in the occurring inflammation. RESULTS: Here we show that even a short-term inhalation of the rod-like CNT induces novel innate immunity-mediated allergic-like airway inflammation in healthy mice. Marked eosinophilia was accompanied by mucus hypersecretion, AHR and the expression of Th2-type cytokines. Exploration of the early events by transcriptomics analysis reveals that a single 4-h exposure to rod-shaped CNT, but not to tangled CNT, causes a radical up-regulation of genes involved in innate immunity and cytokine/chemokine pathways. Mast cells were found to partially regulate the inflammation caused by rod-like CNT, but also alveaolar macrophages play an important role in the early stages. CONCLUSIONS: These observations emphasize the diverse abilities of CNT to impact the immune system, and they should be taken into account for hazard assessment. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12989-014-0048-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-42150162014-11-01 Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation Rydman, Elina M Ilves, Marit Koivisto, Antti J Kinaret, Pia A S Fortino, Vittorio Savinko, Terhi S Lehto, Maili T Pulkkinen, Ville Vippola, Minnamari Hämeri, Kaarle J Matikainen, Sampsa Wolff, Henrik Savolainen, Kai M Greco, Dario Alenius, Harri Part Fibre Toxicol Research BACKGROUND: Carbon nanotubes (CNT) represent a great promise for technological and industrial development but serious concerns on their health effects have also emerged. Rod-shaped CNT are, in fact, able to induce asbestos-like pathogenicity in mice including granuloma formation in abdominal cavity and sub-pleural fibrosis. Exposure to CNT, especially in the occupational context, happens mainly by inhalation. However, little is known about the possible effects of CNT on pulmonary allergic diseases, such as asthma. METHODS: We exposed mice by inhalation to two types of multi-walled CNT, rigid rod-like and flexible tangled CNT, for four hours a day once or on four consecutive days. Early events were monitored immediately and 24 hours after the single inhalation exposure and the four day exposure mimicked an occupational work week. Mast cell deficient mice were used to evaluate the role of mast cells in the occurring inflammation. RESULTS: Here we show that even a short-term inhalation of the rod-like CNT induces novel innate immunity-mediated allergic-like airway inflammation in healthy mice. Marked eosinophilia was accompanied by mucus hypersecretion, AHR and the expression of Th2-type cytokines. Exploration of the early events by transcriptomics analysis reveals that a single 4-h exposure to rod-shaped CNT, but not to tangled CNT, causes a radical up-regulation of genes involved in innate immunity and cytokine/chemokine pathways. Mast cells were found to partially regulate the inflammation caused by rod-like CNT, but also alveaolar macrophages play an important role in the early stages. CONCLUSIONS: These observations emphasize the diverse abilities of CNT to impact the immune system, and they should be taken into account for hazard assessment. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12989-014-0048-2) contains supplementary material, which is available to authorized users. BioMed Central 2014-10-16 /pmc/articles/PMC4215016/ /pubmed/25318534 http://dx.doi.org/10.1186/s12989-014-0048-2 Text en © Rydman et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Rydman, Elina M
Ilves, Marit
Koivisto, Antti J
Kinaret, Pia A S
Fortino, Vittorio
Savinko, Terhi S
Lehto, Maili T
Pulkkinen, Ville
Vippola, Minnamari
Hämeri, Kaarle J
Matikainen, Sampsa
Wolff, Henrik
Savolainen, Kai M
Greco, Dario
Alenius, Harri
Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation
title Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation
title_full Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation
title_fullStr Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation
title_full_unstemmed Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation
title_short Inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation
title_sort inhalation of rod-like carbon nanotubes causes unconventional allergic airway inflammation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215016/
https://www.ncbi.nlm.nih.gov/pubmed/25318534
http://dx.doi.org/10.1186/s12989-014-0048-2
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