Implementation of the CRISPR-Cas9 system in fission yeast

Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence. Here we present expression constructs that use the promoter/leader RNA of RNA (rrk1) and a ribozyme to produce the ta...

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Autores principales: Jacobs, Jake Z., Ciccaglione, Keith M., Tournier, Vincent, Zaratiegui, Mikel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215166/
https://www.ncbi.nlm.nih.gov/pubmed/25352017
http://dx.doi.org/10.1038/ncomms6344
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author Jacobs, Jake Z.
Ciccaglione, Keith M.
Tournier, Vincent
Zaratiegui, Mikel
author_facet Jacobs, Jake Z.
Ciccaglione, Keith M.
Tournier, Vincent
Zaratiegui, Mikel
author_sort Jacobs, Jake Z.
collection PubMed
description Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence. Here we present expression constructs that use the promoter/leader RNA of RNA (rrk1) and a ribozyme to produce the targeting guide RNA. Tobether with constitutive expressio nof Cas9, this system achieves selection-free specific mutagenesis with efficiencies approaching 100%. The rrk1 CRISPR-Cas9 method enables rapid and efficient genome manipulation and unlocks the CRISPR toolset for use in fission yeast.
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spelling pubmed-42151662015-04-29 Implementation of the CRISPR-Cas9 system in fission yeast Jacobs, Jake Z. Ciccaglione, Keith M. Tournier, Vincent Zaratiegui, Mikel Nat Commun Article Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence. Here we present expression constructs that use the promoter/leader RNA of RNA (rrk1) and a ribozyme to produce the targeting guide RNA. Tobether with constitutive expressio nof Cas9, this system achieves selection-free specific mutagenesis with efficiencies approaching 100%. The rrk1 CRISPR-Cas9 method enables rapid and efficient genome manipulation and unlocks the CRISPR toolset for use in fission yeast. 2014-10-29 /pmc/articles/PMC4215166/ /pubmed/25352017 http://dx.doi.org/10.1038/ncomms6344 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Jacobs, Jake Z.
Ciccaglione, Keith M.
Tournier, Vincent
Zaratiegui, Mikel
Implementation of the CRISPR-Cas9 system in fission yeast
title Implementation of the CRISPR-Cas9 system in fission yeast
title_full Implementation of the CRISPR-Cas9 system in fission yeast
title_fullStr Implementation of the CRISPR-Cas9 system in fission yeast
title_full_unstemmed Implementation of the CRISPR-Cas9 system in fission yeast
title_short Implementation of the CRISPR-Cas9 system in fission yeast
title_sort implementation of the crispr-cas9 system in fission yeast
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215166/
https://www.ncbi.nlm.nih.gov/pubmed/25352017
http://dx.doi.org/10.1038/ncomms6344
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