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Guidance for laboratories performing molecular pathology for cancer patients
Molecular testing is becoming an important part of the diagnosis of any patient with cancer. The challenge to laboratories is to meet this need, using reliable methods and processes to ensure that patients receive a timely and accurate report on which their treatment will be based. The aim of this p...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BMJ Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215286/ https://www.ncbi.nlm.nih.gov/pubmed/25012948 http://dx.doi.org/10.1136/jclinpath-2014-202404 |
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author | Cree, Ian A Deans, Zandra Ligtenberg, Marjolijn J L Normanno, Nicola Edsjö, Anders Rouleau, Etienne Solé, Francesc Thunnissen, Erik Timens, Wim Schuuring, Ed Dequeker, Elisabeth Murray, Samuel Dietel, Manfred Groenen, Patricia Van Krieken, J Han |
author_facet | Cree, Ian A Deans, Zandra Ligtenberg, Marjolijn J L Normanno, Nicola Edsjö, Anders Rouleau, Etienne Solé, Francesc Thunnissen, Erik Timens, Wim Schuuring, Ed Dequeker, Elisabeth Murray, Samuel Dietel, Manfred Groenen, Patricia Van Krieken, J Han |
author_sort | Cree, Ian A |
collection | PubMed |
description | Molecular testing is becoming an important part of the diagnosis of any patient with cancer. The challenge to laboratories is to meet this need, using reliable methods and processes to ensure that patients receive a timely and accurate report on which their treatment will be based. The aim of this paper is to provide minimum requirements for the management of molecular pathology laboratories. This general guidance should be augmented by the specific guidance available for different tumour types and tests. Preanalytical considerations are important, and careful consideration of the way in which specimens are obtained and reach the laboratory is necessary. Sample receipt and handling follow standard operating procedures, but some alterations may be necessary if molecular testing is to be performed, for instance to control tissue fixation. DNA and RNA extraction can be standardised and should be checked for quality and quantity of output on a regular basis. The choice of analytical method(s) depends on clinical requirements, desired turnaround time, and expertise available. Internal quality control, regular internal audit of the whole testing process, laboratory accreditation, and continual participation in external quality assessment schemes are prerequisites for delivery of a reliable service. A molecular pathology report should accurately convey the information the clinician needs to treat the patient with sufficient information to allow for correct interpretation of the result. Molecular pathology is developing rapidly, and further detailed evidence-based recommendations are required for many of the topics covered here. |
format | Online Article Text |
id | pubmed-4215286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-42152862014-11-05 Guidance for laboratories performing molecular pathology for cancer patients Cree, Ian A Deans, Zandra Ligtenberg, Marjolijn J L Normanno, Nicola Edsjö, Anders Rouleau, Etienne Solé, Francesc Thunnissen, Erik Timens, Wim Schuuring, Ed Dequeker, Elisabeth Murray, Samuel Dietel, Manfred Groenen, Patricia Van Krieken, J Han J Clin Pathol Best Practice Molecular testing is becoming an important part of the diagnosis of any patient with cancer. The challenge to laboratories is to meet this need, using reliable methods and processes to ensure that patients receive a timely and accurate report on which their treatment will be based. The aim of this paper is to provide minimum requirements for the management of molecular pathology laboratories. This general guidance should be augmented by the specific guidance available for different tumour types and tests. Preanalytical considerations are important, and careful consideration of the way in which specimens are obtained and reach the laboratory is necessary. Sample receipt and handling follow standard operating procedures, but some alterations may be necessary if molecular testing is to be performed, for instance to control tissue fixation. DNA and RNA extraction can be standardised and should be checked for quality and quantity of output on a regular basis. The choice of analytical method(s) depends on clinical requirements, desired turnaround time, and expertise available. Internal quality control, regular internal audit of the whole testing process, laboratory accreditation, and continual participation in external quality assessment schemes are prerequisites for delivery of a reliable service. A molecular pathology report should accurately convey the information the clinician needs to treat the patient with sufficient information to allow for correct interpretation of the result. Molecular pathology is developing rapidly, and further detailed evidence-based recommendations are required for many of the topics covered here. BMJ Publishing Group 2014-11 2014-07-10 /pmc/articles/PMC4215286/ /pubmed/25012948 http://dx.doi.org/10.1136/jclinpath-2014-202404 Text en Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ |
spellingShingle | Best Practice Cree, Ian A Deans, Zandra Ligtenberg, Marjolijn J L Normanno, Nicola Edsjö, Anders Rouleau, Etienne Solé, Francesc Thunnissen, Erik Timens, Wim Schuuring, Ed Dequeker, Elisabeth Murray, Samuel Dietel, Manfred Groenen, Patricia Van Krieken, J Han Guidance for laboratories performing molecular pathology for cancer patients |
title | Guidance for laboratories performing molecular pathology for cancer patients |
title_full | Guidance for laboratories performing molecular pathology for cancer patients |
title_fullStr | Guidance for laboratories performing molecular pathology for cancer patients |
title_full_unstemmed | Guidance for laboratories performing molecular pathology for cancer patients |
title_short | Guidance for laboratories performing molecular pathology for cancer patients |
title_sort | guidance for laboratories performing molecular pathology for cancer patients |
topic | Best Practice |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215286/ https://www.ncbi.nlm.nih.gov/pubmed/25012948 http://dx.doi.org/10.1136/jclinpath-2014-202404 |
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