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Morphological Analysis of Human Induced Pluripotent Stem Cells During Induced Differentiation and Reverse Programming
The fine analysis of cell components during the generation of pluripotent cells and their comparison to bone fide human embryonic stem cells (hESCs) are valuable tools to understand their biological behavior. In this report, human mesenchymal cells (hMSCs) generated from the human ES cell line H9, w...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mary Ann Liebert, Inc.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215385/ https://www.ncbi.nlm.nih.gov/pubmed/25371857 http://dx.doi.org/10.1089/biores.2014.0028 |
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author | Courtot, Anne-Marie Magniez, Aurélie Oudrhiri, Noufissa Féraud, Olivier Bacci, Josette Gobbo, Emilie Proust, Stéphanie Turhan, Ali G. Bennaceur-Griscelli, Annelise |
author_facet | Courtot, Anne-Marie Magniez, Aurélie Oudrhiri, Noufissa Féraud, Olivier Bacci, Josette Gobbo, Emilie Proust, Stéphanie Turhan, Ali G. Bennaceur-Griscelli, Annelise |
author_sort | Courtot, Anne-Marie |
collection | PubMed |
description | The fine analysis of cell components during the generation of pluripotent cells and their comparison to bone fide human embryonic stem cells (hESCs) are valuable tools to understand their biological behavior. In this report, human mesenchymal cells (hMSCs) generated from the human ES cell line H9, were reprogrammed back to induced pluripotent state using Oct-4, Sox2, Nanog, and Lin28 transgenes. Human induced pluripotent stem cells (hIPSCs) were analyzed using electron microscopy and compared with regard to the original hESCs and the hMSCs from which they were derived. This analysis shows that hIPSCs and the original hESCs are morphologically undistinguishable but differ from the hMSCs with respect to the presence of several morphological features of undifferentiated cells at both the cytoplasmic (ribosomes, lipid droplets, glycogen, scarce reticulum) and nuclear levels (features of nuclear plasticity, presence of euchromatin, reticulated nucleoli). We show that hIPSC colonies generated this way presented epithelial aspects with specialized junctions highlighting morphological criteria of the mesenchymal–epithelial transition in cells engaged in a successful reprogramming process. Electron microscopic analysis revealed also specific morphological aspects of partially reprogrammed cells. These results highlight the valuable use of electron microscopy for a better knowledge of the morphological aspects of IPSC and cellular reprogramming. |
format | Online Article Text |
id | pubmed-4215385 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Mary Ann Liebert, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-42153852014-11-04 Morphological Analysis of Human Induced Pluripotent Stem Cells During Induced Differentiation and Reverse Programming Courtot, Anne-Marie Magniez, Aurélie Oudrhiri, Noufissa Féraud, Olivier Bacci, Josette Gobbo, Emilie Proust, Stéphanie Turhan, Ali G. Bennaceur-Griscelli, Annelise Biores Open Access Original Research Articles The fine analysis of cell components during the generation of pluripotent cells and their comparison to bone fide human embryonic stem cells (hESCs) are valuable tools to understand their biological behavior. In this report, human mesenchymal cells (hMSCs) generated from the human ES cell line H9, were reprogrammed back to induced pluripotent state using Oct-4, Sox2, Nanog, and Lin28 transgenes. Human induced pluripotent stem cells (hIPSCs) were analyzed using electron microscopy and compared with regard to the original hESCs and the hMSCs from which they were derived. This analysis shows that hIPSCs and the original hESCs are morphologically undistinguishable but differ from the hMSCs with respect to the presence of several morphological features of undifferentiated cells at both the cytoplasmic (ribosomes, lipid droplets, glycogen, scarce reticulum) and nuclear levels (features of nuclear plasticity, presence of euchromatin, reticulated nucleoli). We show that hIPSC colonies generated this way presented epithelial aspects with specialized junctions highlighting morphological criteria of the mesenchymal–epithelial transition in cells engaged in a successful reprogramming process. Electron microscopic analysis revealed also specific morphological aspects of partially reprogrammed cells. These results highlight the valuable use of electron microscopy for a better knowledge of the morphological aspects of IPSC and cellular reprogramming. Mary Ann Liebert, Inc. 2014-10-01 /pmc/articles/PMC4215385/ /pubmed/25371857 http://dx.doi.org/10.1089/biores.2014.0028 Text en Copyright 2014, Mary Ann Liebert, Inc. |
spellingShingle | Original Research Articles Courtot, Anne-Marie Magniez, Aurélie Oudrhiri, Noufissa Féraud, Olivier Bacci, Josette Gobbo, Emilie Proust, Stéphanie Turhan, Ali G. Bennaceur-Griscelli, Annelise Morphological Analysis of Human Induced Pluripotent Stem Cells During Induced Differentiation and Reverse Programming |
title | Morphological Analysis of Human Induced Pluripotent Stem Cells During Induced Differentiation and Reverse Programming |
title_full | Morphological Analysis of Human Induced Pluripotent Stem Cells During Induced Differentiation and Reverse Programming |
title_fullStr | Morphological Analysis of Human Induced Pluripotent Stem Cells During Induced Differentiation and Reverse Programming |
title_full_unstemmed | Morphological Analysis of Human Induced Pluripotent Stem Cells During Induced Differentiation and Reverse Programming |
title_short | Morphological Analysis of Human Induced Pluripotent Stem Cells During Induced Differentiation and Reverse Programming |
title_sort | morphological analysis of human induced pluripotent stem cells during induced differentiation and reverse programming |
topic | Original Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215385/ https://www.ncbi.nlm.nih.gov/pubmed/25371857 http://dx.doi.org/10.1089/biores.2014.0028 |
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