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Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response

Dematiaceous fungi constitute a large and heterogeneous group, characterized by having a dark pigment, the dihydroxynaftalen melanin—DHN, inside their cell walls. In nature they are found mainly as soil microbiota or decomposing organic matter, and are spread in tropical and subtropical regions. The...

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Autores principales: Burjack, Juliana R., Santana-Filho, Arquimedes P., Ruthes, Andrea C., Riter, Daniel S., Vicente, Vania A., Alvarenga, Larissa M., Sassaki, Guilherme L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215789/
https://www.ncbi.nlm.nih.gov/pubmed/25401093
http://dx.doi.org/10.3389/fcimb.2014.00153
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author Burjack, Juliana R.
Santana-Filho, Arquimedes P.
Ruthes, Andrea C.
Riter, Daniel S.
Vicente, Vania A.
Alvarenga, Larissa M.
Sassaki, Guilherme L.
author_facet Burjack, Juliana R.
Santana-Filho, Arquimedes P.
Ruthes, Andrea C.
Riter, Daniel S.
Vicente, Vania A.
Alvarenga, Larissa M.
Sassaki, Guilherme L.
author_sort Burjack, Juliana R.
collection PubMed
description Dematiaceous fungi constitute a large and heterogeneous group, characterized by having a dark pigment, the dihydroxynaftalen melanin—DHN, inside their cell walls. In nature they are found mainly as soil microbiota or decomposing organic matter, and are spread in tropical and subtropical regions. The fungus Fonsecaea monophora causes chromoblastomycosis in humans, and possesses essential mechanisms that may enhance pathogenicity, proliferation and dissemination inside the host. Glycoconjugates confer important properties to these pathogenic microorganisms. In this work, structural characterization of glycan structures present in two different strains of F. monophora MMHC82 and FE5p4, from clinical and environmental origins, respectively, was performed. Each one were grown on Minimal Medium (MM) and Czapeck-Dox (CD) medium, and the water soluble cell wall glycoconjugates and exopolysaccharides (EPS) were evaluated by NMR, methylation and principal component analysis (PCA). By combining the methylation and 2D NMR analyses, it was possible to visualize the glycosidic profiles of the complex carbohydrate mixtures. Significant differences were observed in β-D-Galf-(1→5) and (1→6) linkages, α- and β-D-Glcp-(1→3), (1→4), and (1→6) units, as well as in α-D-Manp. PCA from (1)H-NMR data showed that MMHC82 from CD medium showed a higher variation in the cell wall carbohydrates, mainly related to O-2 substituted β-D-Galf (δ 106.0/5.23 and δ 105.3/5.23) units. In order to investigate the antigenic response of the glycoconjugates, these were screened against serum from chromoblastomycosis patients. The antigen which contained the cell wall of MMHC82 grown in MM had β-D-Manp units that promoted higher antigenic response. The distribution of these fungal species in nature and the knowledge of how cell wall polysaccharides and glycoconjugates structure vary, may contribute to the better understanding and the elucidation of the pathology caused by this fungus.
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spelling pubmed-42157892014-11-14 Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response Burjack, Juliana R. Santana-Filho, Arquimedes P. Ruthes, Andrea C. Riter, Daniel S. Vicente, Vania A. Alvarenga, Larissa M. Sassaki, Guilherme L. Front Cell Infect Microbiol Microbiology Dematiaceous fungi constitute a large and heterogeneous group, characterized by having a dark pigment, the dihydroxynaftalen melanin—DHN, inside their cell walls. In nature they are found mainly as soil microbiota or decomposing organic matter, and are spread in tropical and subtropical regions. The fungus Fonsecaea monophora causes chromoblastomycosis in humans, and possesses essential mechanisms that may enhance pathogenicity, proliferation and dissemination inside the host. Glycoconjugates confer important properties to these pathogenic microorganisms. In this work, structural characterization of glycan structures present in two different strains of F. monophora MMHC82 and FE5p4, from clinical and environmental origins, respectively, was performed. Each one were grown on Minimal Medium (MM) and Czapeck-Dox (CD) medium, and the water soluble cell wall glycoconjugates and exopolysaccharides (EPS) were evaluated by NMR, methylation and principal component analysis (PCA). By combining the methylation and 2D NMR analyses, it was possible to visualize the glycosidic profiles of the complex carbohydrate mixtures. Significant differences were observed in β-D-Galf-(1→5) and (1→6) linkages, α- and β-D-Glcp-(1→3), (1→4), and (1→6) units, as well as in α-D-Manp. PCA from (1)H-NMR data showed that MMHC82 from CD medium showed a higher variation in the cell wall carbohydrates, mainly related to O-2 substituted β-D-Galf (δ 106.0/5.23 and δ 105.3/5.23) units. In order to investigate the antigenic response of the glycoconjugates, these were screened against serum from chromoblastomycosis patients. The antigen which contained the cell wall of MMHC82 grown in MM had β-D-Manp units that promoted higher antigenic response. The distribution of these fungal species in nature and the knowledge of how cell wall polysaccharides and glycoconjugates structure vary, may contribute to the better understanding and the elucidation of the pathology caused by this fungus. Frontiers Media S.A. 2014-10-31 /pmc/articles/PMC4215789/ /pubmed/25401093 http://dx.doi.org/10.3389/fcimb.2014.00153 Text en Copyright © 2014 Burjack, Santana-Filho, Ruthes, Riter, Vicente, Alvarenga and Sassaki. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Burjack, Juliana R.
Santana-Filho, Arquimedes P.
Ruthes, Andrea C.
Riter, Daniel S.
Vicente, Vania A.
Alvarenga, Larissa M.
Sassaki, Guilherme L.
Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response
title Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response
title_full Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response
title_fullStr Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response
title_full_unstemmed Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response
title_short Glycan analysis of Fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response
title_sort glycan analysis of fonsecaea monophora from clinical and environmental origins reveals different structural profile and human antigenic response
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215789/
https://www.ncbi.nlm.nih.gov/pubmed/25401093
http://dx.doi.org/10.3389/fcimb.2014.00153
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