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Steady-State Acceptor Fluorescence Anisotropy Imaging under Evanescent Excitation for Visualisation of FRET at the Plasma Membrane

We present a novel imaging system combining total internal reflection fluorescence (TIRF) microscopy with measurement of steady-state acceptor fluorescence anisotropy in order to perform live cell Förster Resonance Energy Transfer (FRET) imaging at the plasma membrane. We compare directly the imagin...

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Detalles Bibliográficos
Autores principales:	Devauges, Viviane, Matthews, Daniel R., Aluko, Justin, Nedbal, Jakub, Levitt, James A., Poland, Simon P., Coban, Oana, Weitsman, Gregory, Monypenny, James, Ng, Tony, Ameer-Beg, Simon M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215982/ https://www.ncbi.nlm.nih.gov/pubmed/25360776 http://dx.doi.org/10.1371/journal.pone.0110695

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