Rapid Restriction Enzyme-Free Cloning of PCR Products: A High-Throughput Method Applicable for Library Construction

Herein, we describe a novel cloning strategy for PCR-amplified DNA which employs the type IIs restriction endonuclease BsaI to create a linearized vector with four base-long 5′-overhangs, and T4 DNA polymerase treatment of the insert in presence of a single dNTP to create vector-compatible four base...

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Detalles Bibliográficos
Autores principales: Chaudhary, Vijay K., Shrivastava, Nimisha, Verma, Vaishali, Das, Shilpi, Kaur, Charanpreet, Grover, Payal, Gupta, Amita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4216109/
https://www.ncbi.nlm.nih.gov/pubmed/25360695
http://dx.doi.org/10.1371/journal.pone.0111538

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4216109/
https://www.ncbi.nlm.nih.gov/pubmed/25360695
http://dx.doi.org/10.1371/journal.pone.0111538

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