Cargando…

Rapid Restriction Enzyme-Free Cloning of PCR Products: A High-Throughput Method Applicable for Library Construction

Herein, we describe a novel cloning strategy for PCR-amplified DNA which employs the type IIs restriction endonuclease BsaI to create a linearized vector with four base-long 5′-overhangs, and T4 DNA polymerase treatment of the insert in presence of a single dNTP to create vector-compatible four base...

Descripción completa

Detalles Bibliográficos
Autores principales:	Chaudhary, Vijay K., Shrivastava, Nimisha, Verma, Vaishali, Das, Shilpi, Kaur, Charanpreet, Grover, Payal, Gupta, Amita
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4216109/ https://www.ncbi.nlm.nih.gov/pubmed/25360695 http://dx.doi.org/10.1371/journal.pone.0111538

Ejemplares similares

A Novel Helper Phage Enabling Construction of Genome-Scale ORF-Enriched Phage Display Libraries
por: Gupta, Amita, et al.
Publicado: (2013)

Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection
por: Verma, Vaishali, et al.
Publicado: (2018)

An efficient ORF selection system for DNA fragment libraries based on split beta-lactamase complementation
por: Verma, Vaishali, et al.
Publicado: (2020)

One step construction of PCR mutagenized libraries for genetic analysis by recombination cloning
por: Khalil, Al-Muataz, et al.
Publicado: (2007)

Restriction Enzyme Body Doubles and PCR Cloning: On the General Use of Type IIS Restriction Enzymes for Cloning
por: Tóth, Eszter, et al.
Publicado: (2014)

Recombinant expression library of Pyrococcus furiosus constructed by high-throughput cloning: a useful tool for functional and structural genomics
por: Yuan, Hui, et al.
Publicado: (2015)

Streamlined high-throughput cloning protocol to generate arrayed mutant libraries
por: Sun, Kerry T., et al.
Publicado: (2022)

Cloning antibodies from single cells in pooled sequence libraries by selective PCR
por: Horns, Felix, et al.
Publicado: (2020)

A high-throughput strategy for screening of bacterial artificial chromosome libraries and anchoring of clones on a genetic map constructed with single nucleotide polymorphisms
por: Luo, Ming-Cheng, et al.
Publicado: (2009)

Evaluation of a Pooled Strategy for High-Throughput Sequencing of Cosmid Clones from Metagenomic Libraries
por: Lam, Kathy N., et al.
Publicado: (2014)

High throughput sequencing analysis of RNA libraries reveals the influences of initial library and PCR methods on SELEX efficiency
por: Takahashi, Mayumi, et al.
Publicado: (2016)

Ribosomal Binding Site Switching: An Effective Strategy for High-Throughput Cloning Constructions
por: Hu, Yangbo, et al.
Publicado: (2012)

Hackflex: low-cost, high-throughput, Illumina Nextera Flex library construction
por: Gaio, Daniela, et al.
Publicado: (2022)

A new implementation of high-throughput five-dimensional clone pooling strategy for BAC library screening
por: You, Frank M, et al.
Publicado: (2010)

A New High-Throughput Screening Method to Detect Antimicrobial Volatiles from Metagenomic Clone Libraries
por: Stocker, Franz, et al.
Publicado: (2020)

Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes
por: Fischer, Matthew D., et al.
Publicado: (2018)

M. tuberculosis Secretory Protein ESAT-6 Induces Metabolic Flux Perturbations to Drive Foamy Macrophage Differentiation
por: Singh, Varshneya, et al.
Publicado: (2015)

Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, Lates calcarifer
por: Wang, Chun Ming, et al.
Publicado: (2008)

Restriction Site-dependent PCR: An Efficient Technique for Fast Cloning of New Genes of Microorganisms
por: Jiang, Yu, et al.
Publicado: (2007)

BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.)
por: Schulte, Daniela, et al.
Publicado: (2011)

Construction and analysis of a leaf cDNA library from cold stressed rubber tree clones
por: Da Silva, Carla, et al.
Publicado: (2011)

Cloning of Insertion Site Flanking Sequence and Construction of Transfer DNA Insert Mutant Library in Stylosanthes Colletotrichum
por: Chen, Helong, et al.
Publicado: (2014)

Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method
por: Meng, Fan, et al.
Publicado: (2023)

Single Tube, High Throughput Cloning of Inverted Repeat Constructs for Double-Stranded RNA Expression
por: Hauge, Brian, et al.
Publicado: (2009)

PrecisePrimer: an easy-to-use web server for designing PCR primers for DNA library cloning and DNA shuffling
por: Pauthenier, Cyrille, et al.
Publicado: (2014)

A high-throughput, restriction-free cloning and screening strategy based on ccdB-gene replacement
por: Lund, Bjarte Aarmo, et al.
Publicado: (2014)

Inverse Fusion PCR Cloning
por: Spiliotis, Markus
Publicado: (2012)

ECG Library
por: Schuchert, Vaishali Dixit
Publicado: (2006)

A PCR-free rapid protocol for one-pot construction of highly diverse genetic libraries
por: Woolley, Michael, et al.
Publicado: (2022)

Construction of non-canonical PAM-targeting adenosine base editors by restriction enzyme-free DNA cloning using CRISPR-Cas9
por: Jeong, You Kyeong, et al.
Publicado: (2019)

Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems
por: Fukunaga, Keisuke, et al.
Publicado: (2012)

Establishment of Okadaic Acid Resistant Cell Clones using CDNA Library Expression Cloning
por: Sandal, Tone, et al.
Publicado: (2001)

Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning
por: Fujii, Wataru, et al.
Publicado: (2013)

Construction of a High Efficiency PCR Products Cloning T Vector Using pGEM-5zf (+)
por: Zhao, Yaofeng, et al.
Publicado: (2009)

Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis
por: Qin, Huajun, et al.
Publicado: (2008)

A Biobrick Library for Cloning Custom Eukaryotic Plasmids
por: Constante, Marco, et al.
Publicado: (2011)

Construction and optimization of an efficient amplification method of a random ssDNA library by asymmetric emulsion PCR
por: Shao, Keke, et al.
Publicado: (2016)

A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries
por: Tsoumpeli, Maria T., et al.
Publicado: (2022)

A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system
por: Bhat, Sushant, et al.
Publicado: (2020)

Pattern analysis approach reveals restriction enzyme cutting abnormalities and other cDNA library construction artifacts using raw EST data
por: Zhou, Sun, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_fub7uqdsrs3lpotae0fi8ls95m