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Children with severe early childhood caries: streptococci genetic strains within carious and white spot lesions
BACKGROUND AND OBJECTIVES: Mutans streptococci (MS) are one of the major microbiological determinants of dental caries. The objectives of this study are to identify distinct MS and non-MS streptococci strains that are located at carious sites and non-carious enamel surfaces in children with severe e...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Co-Action Publishing
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4216391/ https://www.ncbi.nlm.nih.gov/pubmed/25405004 http://dx.doi.org/10.3402/jom.v6.25805 |
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author | Gilbert, Kenneth Joseph, Raphael Vo, Alex Patel, Trusha Chaudhry, Samiya Nguyen, Uyen Trevor, Amy Robinson, Erica Campbell, Margaret McLennan, John Houran, Farielle Wong, Tristan Flann, Kendra Wages, Melissa Palmer, Elizabeth A. Peterson, John Engle, John Maier, Tom Machida, Curtis A. |
author_facet | Gilbert, Kenneth Joseph, Raphael Vo, Alex Patel, Trusha Chaudhry, Samiya Nguyen, Uyen Trevor, Amy Robinson, Erica Campbell, Margaret McLennan, John Houran, Farielle Wong, Tristan Flann, Kendra Wages, Melissa Palmer, Elizabeth A. Peterson, John Engle, John Maier, Tom Machida, Curtis A. |
author_sort | Gilbert, Kenneth |
collection | PubMed |
description | BACKGROUND AND OBJECTIVES: Mutans streptococci (MS) are one of the major microbiological determinants of dental caries. The objectives of this study are to identify distinct MS and non-MS streptococci strains that are located at carious sites and non-carious enamel surfaces in children with severe early childhood caries (S-ECC), and assess if cariogenic MS and non-cariogenic streptococci might independently exist as primary bacterial strains on distinct sites within the dentition of individual children. DESIGN: Dental plaque from children (N=20; aged 3–6) with S-ECC was collected from carious lesions (CLs), white spot lesions (WSLs) and non-carious enamel surfaces. Streptococcal isolates (N=10–20) from each site were subjected to polymerase chain reaction (PCR) to identify MS, and arbitrarily primed-PCR for assignment of genetic strains. Primary strains were identified as ≥50% of the total isolates surveyed at any site. In several cases, strains were characterized for acidurity using ATP-driven bioluminescence and subjected to PCR-determination of potential MS virulence products. Identification of non-MS was determined by 16S rRNA gene sequencing. RESULTS: Sixty-four independent MS or non-MS streptococcal strains were identified. All children contained 1–6 strains. In many patients (N=11), single primary MS strains were identified throughout the dentition. In other patients (N=4), primary MS strains were identified within CLs that were distinct from primary strains found on enamel. Streptococcus gordonii strains were identified as primary strains on enamel or WSLs in four children, and in general were less aciduric than MS strains. CONCLUSIONS: Many children with S-ECC contained only a single primary MS strain that was present in both carious and non-carious sites. In some cases, MS and non-cariogenic S. gordonii strains were found to independently exist as dominant strains at different locations within the dentition of individual children, and the aciduric potential of these strains may influence susceptibility in the development of CLs. |
format | Online Article Text |
id | pubmed-4216391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Co-Action Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-42163912014-11-17 Children with severe early childhood caries: streptococci genetic strains within carious and white spot lesions Gilbert, Kenneth Joseph, Raphael Vo, Alex Patel, Trusha Chaudhry, Samiya Nguyen, Uyen Trevor, Amy Robinson, Erica Campbell, Margaret McLennan, John Houran, Farielle Wong, Tristan Flann, Kendra Wages, Melissa Palmer, Elizabeth A. Peterson, John Engle, John Maier, Tom Machida, Curtis A. J Oral Microbiol Original Article BACKGROUND AND OBJECTIVES: Mutans streptococci (MS) are one of the major microbiological determinants of dental caries. The objectives of this study are to identify distinct MS and non-MS streptococci strains that are located at carious sites and non-carious enamel surfaces in children with severe early childhood caries (S-ECC), and assess if cariogenic MS and non-cariogenic streptococci might independently exist as primary bacterial strains on distinct sites within the dentition of individual children. DESIGN: Dental plaque from children (N=20; aged 3–6) with S-ECC was collected from carious lesions (CLs), white spot lesions (WSLs) and non-carious enamel surfaces. Streptococcal isolates (N=10–20) from each site were subjected to polymerase chain reaction (PCR) to identify MS, and arbitrarily primed-PCR for assignment of genetic strains. Primary strains were identified as ≥50% of the total isolates surveyed at any site. In several cases, strains were characterized for acidurity using ATP-driven bioluminescence and subjected to PCR-determination of potential MS virulence products. Identification of non-MS was determined by 16S rRNA gene sequencing. RESULTS: Sixty-four independent MS or non-MS streptococcal strains were identified. All children contained 1–6 strains. In many patients (N=11), single primary MS strains were identified throughout the dentition. In other patients (N=4), primary MS strains were identified within CLs that were distinct from primary strains found on enamel. Streptococcus gordonii strains were identified as primary strains on enamel or WSLs in four children, and in general were less aciduric than MS strains. CONCLUSIONS: Many children with S-ECC contained only a single primary MS strain that was present in both carious and non-carious sites. In some cases, MS and non-cariogenic S. gordonii strains were found to independently exist as dominant strains at different locations within the dentition of individual children, and the aciduric potential of these strains may influence susceptibility in the development of CLs. Co-Action Publishing 2014-10-29 /pmc/articles/PMC4216391/ /pubmed/25405004 http://dx.doi.org/10.3402/jom.v6.25805 Text en © 2014 Kenneth Gilbert et al. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Gilbert, Kenneth Joseph, Raphael Vo, Alex Patel, Trusha Chaudhry, Samiya Nguyen, Uyen Trevor, Amy Robinson, Erica Campbell, Margaret McLennan, John Houran, Farielle Wong, Tristan Flann, Kendra Wages, Melissa Palmer, Elizabeth A. Peterson, John Engle, John Maier, Tom Machida, Curtis A. Children with severe early childhood caries: streptococci genetic strains within carious and white spot lesions |
title | Children with severe early childhood caries: streptococci genetic strains within carious and white spot lesions |
title_full | Children with severe early childhood caries: streptococci genetic strains within carious and white spot lesions |
title_fullStr | Children with severe early childhood caries: streptococci genetic strains within carious and white spot lesions |
title_full_unstemmed | Children with severe early childhood caries: streptococci genetic strains within carious and white spot lesions |
title_short | Children with severe early childhood caries: streptococci genetic strains within carious and white spot lesions |
title_sort | children with severe early childhood caries: streptococci genetic strains within carious and white spot lesions |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4216391/ https://www.ncbi.nlm.nih.gov/pubmed/25405004 http://dx.doi.org/10.3402/jom.v6.25805 |
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