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Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus

The endemicity of highly pathogenic avian influenza (HPAI) A(H5N1) viruses in Asia has led to the generation of reassortant H5 strains with novel gene constellations. A newly emerged HPAI A(H5N8) virus caused poultry outbreaks in the Republic of Korea in 2014. Because newly emerging high-pathogenici...

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Autores principales: Kim, Young-Il, Pascua, Philippe Noriel Q, Kwon, Hyeok-Il, Lim, Gyo-Jin, Kim, Eun-Ha, Yoon, Sun-Woo, Park, Su-Jin, Kim, Se Mi, Choi, Eun-Ji, Si, Young-Jae, Lee, Ok-Jun, Shim, Woo-Sub, Kim, Si-Wook, Mo, In-Pil, Bae, Yeonji, Lim, Yong Taik, Sung, Moon Hee, Kim, Chul-Joong, Webby, Richard J, Webster, Robert G, Choi, Young Ki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4217095/
https://www.ncbi.nlm.nih.gov/pubmed/26038499
http://dx.doi.org/10.1038/emi.2014.75
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author Kim, Young-Il
Pascua, Philippe Noriel Q
Kwon, Hyeok-Il
Lim, Gyo-Jin
Kim, Eun-Ha
Yoon, Sun-Woo
Park, Su-Jin
Kim, Se Mi
Choi, Eun-Ji
Si, Young-Jae
Lee, Ok-Jun
Shim, Woo-Sub
Kim, Si-Wook
Mo, In-Pil
Bae, Yeonji
Lim, Yong Taik
Sung, Moon Hee
Kim, Chul-Joong
Webby, Richard J
Webster, Robert G
Choi, Young Ki
author_facet Kim, Young-Il
Pascua, Philippe Noriel Q
Kwon, Hyeok-Il
Lim, Gyo-Jin
Kim, Eun-Ha
Yoon, Sun-Woo
Park, Su-Jin
Kim, Se Mi
Choi, Eun-Ji
Si, Young-Jae
Lee, Ok-Jun
Shim, Woo-Sub
Kim, Si-Wook
Mo, In-Pil
Bae, Yeonji
Lim, Yong Taik
Sung, Moon Hee
Kim, Chul-Joong
Webby, Richard J
Webster, Robert G
Choi, Young Ki
author_sort Kim, Young-Il
collection PubMed
description The endemicity of highly pathogenic avian influenza (HPAI) A(H5N1) viruses in Asia has led to the generation of reassortant H5 strains with novel gene constellations. A newly emerged HPAI A(H5N8) virus caused poultry outbreaks in the Republic of Korea in 2014. Because newly emerging high-pathogenicity H5 viruses continue to pose public health risks, it is imperative that their pathobiological properties be examined. Here, we characterized A/mallard duck/Korea/W452/2014 (MDk/W452(H5N8)), a representative virus, and evaluated its pathogenic and pandemic potential in various animal models. We found that MDk/W452(H5N8), which originated from the reassortment of wild bird viruses harbored by migratory waterfowl in eastern China, replicated systemically and was lethal in chickens, but appeared to be attenuated, albeit efficiently transmitted, in ducks. Despite predominant attachment to avian-like virus receptors, MDk/W452(H5N8) also exhibited detectable human virus-like receptor binding and replicated in human respiratory tract tissues. In mice, MDk/W452(H5N8) was moderately pathogenic and had limited tissue tropism relative to previous HPAI A(H5N1) viruses. It also induced moderate nasal wash titers in inoculated ferrets; additionally, it was recovered in extrapulmonary tissues and one of three direct-contact ferrets seroconverted without shedding. Moreover, domesticated cats appeared to be more susceptible than dogs to virus infection. With their potential to become established in ducks, continued circulation of A(H5N8) viruses could alter the genetic evolution of pre-existing avian poultry strains. Overall, detailed virological investigation remains a necessity given the capacity of H5 viruses to evolve to cause human illness with few changes in the viral genome.
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spelling pubmed-42170952014-11-03 Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus Kim, Young-Il Pascua, Philippe Noriel Q Kwon, Hyeok-Il Lim, Gyo-Jin Kim, Eun-Ha Yoon, Sun-Woo Park, Su-Jin Kim, Se Mi Choi, Eun-Ji Si, Young-Jae Lee, Ok-Jun Shim, Woo-Sub Kim, Si-Wook Mo, In-Pil Bae, Yeonji Lim, Yong Taik Sung, Moon Hee Kim, Chul-Joong Webby, Richard J Webster, Robert G Choi, Young Ki Emerg Microbes Infect Original Article The endemicity of highly pathogenic avian influenza (HPAI) A(H5N1) viruses in Asia has led to the generation of reassortant H5 strains with novel gene constellations. A newly emerged HPAI A(H5N8) virus caused poultry outbreaks in the Republic of Korea in 2014. Because newly emerging high-pathogenicity H5 viruses continue to pose public health risks, it is imperative that their pathobiological properties be examined. Here, we characterized A/mallard duck/Korea/W452/2014 (MDk/W452(H5N8)), a representative virus, and evaluated its pathogenic and pandemic potential in various animal models. We found that MDk/W452(H5N8), which originated from the reassortment of wild bird viruses harbored by migratory waterfowl in eastern China, replicated systemically and was lethal in chickens, but appeared to be attenuated, albeit efficiently transmitted, in ducks. Despite predominant attachment to avian-like virus receptors, MDk/W452(H5N8) also exhibited detectable human virus-like receptor binding and replicated in human respiratory tract tissues. In mice, MDk/W452(H5N8) was moderately pathogenic and had limited tissue tropism relative to previous HPAI A(H5N1) viruses. It also induced moderate nasal wash titers in inoculated ferrets; additionally, it was recovered in extrapulmonary tissues and one of three direct-contact ferrets seroconverted without shedding. Moreover, domesticated cats appeared to be more susceptible than dogs to virus infection. With their potential to become established in ducks, continued circulation of A(H5N8) viruses could alter the genetic evolution of pre-existing avian poultry strains. Overall, detailed virological investigation remains a necessity given the capacity of H5 viruses to evolve to cause human illness with few changes in the viral genome. Nature Publishing Group 2014-10 2014-10-22 /pmc/articles/PMC4217095/ /pubmed/26038499 http://dx.doi.org/10.1038/emi.2014.75 Text en Copyright © 2014 Shanghai Shangyixun Cultural Communication Co., Ltd http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
Kim, Young-Il
Pascua, Philippe Noriel Q
Kwon, Hyeok-Il
Lim, Gyo-Jin
Kim, Eun-Ha
Yoon, Sun-Woo
Park, Su-Jin
Kim, Se Mi
Choi, Eun-Ji
Si, Young-Jae
Lee, Ok-Jun
Shim, Woo-Sub
Kim, Si-Wook
Mo, In-Pil
Bae, Yeonji
Lim, Yong Taik
Sung, Moon Hee
Kim, Chul-Joong
Webby, Richard J
Webster, Robert G
Choi, Young Ki
Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus
title Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus
title_full Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus
title_fullStr Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus
title_full_unstemmed Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus
title_short Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus
title_sort pathobiological features of a novel, highly pathogenic avian influenza a(h5n8) virus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4217095/
https://www.ncbi.nlm.nih.gov/pubmed/26038499
http://dx.doi.org/10.1038/emi.2014.75
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