Optimized fluorescent labeling to identify memory B cells specific for Neisseria meningitidis serogroup B vaccine antigens ex vivo

Antigen-specific memory B cells generate anamnestic responses and high affinity antibodies upon re-exposure to pathogens. Attempts to isolate rare antigen-specific memory B cells for in-depth functional analysis at the single-cell level have been hindered by the lack of tools with adequate sensitivi...

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Detalles Bibliográficos
Autores principales: Nair, Nitya, Buti, Ludovico, Faenzi, Elisa, Buricchi, Francesca, Nuti, Sandra, Sammicheli, Chiara, Tavarini, Simona, Popp, Maximilian WL, Ploegh, Hidde, Berti, Francesco, Pizza, Mariagrazia, Castellino, Flora, Finco, Oretta, Rappuoli, Rino, Del Giudice, Giuseppe, Galli, Grazia, Bardelli, Monia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4217542/
https://www.ncbi.nlm.nih.gov/pubmed/25400913
http://dx.doi.org/10.1002/iid3.3

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