Cargando…

Quantifying mRNA and MicroRNA with qPCR in Cervical Carcinogenesis: A Validation of Reference Genes to Ensure Accurate Data

A number of recent studies have catalogued global gene expression patterns in a panel of normal, tumoral cervical tissues so that potential biomarkers can be identified. The qPCR has been one of the most widely used technologies for detecting these potential biomarkers. However, few studies have inv...

Descripción completa

Detalles Bibliográficos
Autores principales:	Leitão, Maria da Conceição Gomes, Coimbra, Eliane Campos, de Lima, Rita de Cássia Pereira, Guimarães, Mariléa de Lima, Heráclio, Sandra de Andrade, Silva Neto, Jacinto da Costa, de Freitas, Antonio Carlos
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4217744/ https://www.ncbi.nlm.nih.gov/pubmed/25365304 http://dx.doi.org/10.1371/journal.pone.0111021

Ejemplares similares

Validation of reference genes aiming accurate normalization of qPCR data in soybean upon nematode parasitism and insect attack
por: Miranda, Vívian de Jesus, et al.
Publicado: (2013)

Ensuring vaccine safety: Case studies of falsified influenza vaccines detected in Brazil
por: da Costa, Jaline Alves Cabral, et al.
Publicado: (2023)

RNA-based qPCR as a tool to quantify and to characterize dual-species biofilms
por: Magalhães, Andreia Patrícia, et al.
Publicado: (2019)

Comparison between qPCR and RNA-seq reveals challenges of quantifying HLA expression
por: Aguiar, Vitor R. C., et al.
Publicado: (2023)

Pterostilbene and Probiotic Complex in Chemoprevention of Putative Precursor Lesions for Colorectal Cancer in an Experimental Model of Intestinal Carcinogenesis with 1,2-Dimethylhydrazine
por: Barreira, Márcio Alencar, et al.
Publicado: (2023)

Reduced Expression of IL-1β and IL-18 Proinflammatory Interleukins Increases the Risk of Developing Cervical Cancer
por: Matamoros, Jose Anibal, et al.
Publicado: (2019)

Effect of Demographics and Time to Sample Processing on the qPCR Detection of Pathogenic Leptospira spp. from Human Samples in the National Reference Laboratory for Leptospirosis, Brazil
por: Neris, Romulo Leão Silva, et al.
Publicado: (2023)

A Facile and Specific Assay for Quantifying MicroRNA by an Optimized RT-qPCR Approach
por: Mei, Qian, et al.
Publicado: (2012)

Postmenopausal Osteoporosis reference genes for qPCR expression assays
por: de Lima, Camilla Albertina Dantas, et al.
Publicado: (2019)

Modulation of Cell Sialoglycophenotype: A Stylish Mechanism Adopted by Trypanosoma cruzi to Ensure Its Persistence in the Infected Host
por: Freire-de-Lima, Leonardo, et al.
Publicado: (2016)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

Reference Genes for qPCR Analysis in Resin-Tapped Adult Slash Pine As a Tool to Address the Molecular Basis of Commercial Resinosis
por: de Lima, Júlio C., et al.
Publicado: (2016)

The role of TNF-α and NFkβ in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine
por: Barreira, Márcio Alencar, et al.
Publicado: (2023)

Corrigendum: Reference Genes for qPCR Analysis in Resin-Tapped Adult Slash Pine As a Tool to Address the Molecular Basis of Commercial Resinosis
por: de Lima, Júlio C., et al.
Publicado: (2018)

Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification
por: Androvic, Peter, et al.
Publicado: (2017)

A sequence element that tunes E. coli tRNA(GGC)(Ala) to ensure accurate decoding
por: Ledoux, Sarah, et al.
Publicado: (2009)

Selection and testing of reference genes for accurate RT-qPCR in rice seedlings under iron toxicity
por: dos Santos, Fabiane Igansi de Castro, et al.
Publicado: (2018)

I Choose You: Selecting Accurate Reference Genes for qPCR Expression Analysis in Reproductive Tissues in Arabidopsis thaliana
por: Ferreira, Maria João, et al.
Publicado: (2023)

Parasite load evaluation by qPCR and blood culture in Chagas disease and HIV co-infected patients under antiretroviral therapy
por: Marcon, Gláucia Elisete Barbosa, et al.
Publicado: (2022)

Ensuring accurate testing for human immunodeficiency virus in Myanmar
por: Kyaw, Latt Latt, et al.
Publicado: (2015)

A novel and universal method for microRNA RT-qPCR data normalization
por: Mestdagh, Pieter, et al.
Publicado: (2009)

Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
por: Nunes, Bruno Tardelli Diniz, et al.
Publicado: (2019)

Effectiveness of an action-oriented educational intervention in ensuring long term improvement of knowledge, attitudes and practices of community health workers in maternal and infant health: a randomized controlled study
por: Melo e Lima, Tereza Rebecca de, et al.
Publicado: (2018)

A new and accurate qPCR protocol to detect plant pathogenic bacteria of the genus ‘Candidatus Liberibacter’ in plants and insects
por: de Chaves, María Quintana-González, et al.
Publicado: (2023)

The Inhibition and Variability of Two Different RT-qPCR Assays Used for Quantifying SARS-CoV-2 RNA in Wastewater
por: Scott, George, et al.
Publicado: (2023)

Validating Internal Control Genes for the Accurate Normalization of qPCR Expression Analysis of the Novel Model Plant Setaria viridis
por: Lambret-Frotté, Julia, et al.
Publicado: (2015)

What is the Role of SNORA42 in Carcinogenesis? A Systematic Review
por: Barbosa, Eldevan da Silva, et al.
Publicado: (2023)

RNA Vaccines: Yeast as a Novel Antigen Vehicle
por: Silva, Anna Jéssica Duarte, et al.
Publicado: (2023)

Innovative microRNA quantification by qPCR
por: Le, Minh Ngoc, et al.
Publicado: (2023)

RT-qPCR-based pool testing for the diagnosis of COVID-19
por: Sato, Hugo Itaru, et al.
Publicado: (2023)

MicroRNA deregulation in nonalcoholic steatohepatitis-associated liver carcinogenesis
por: de Conti, Aline, et al.
Publicado: (2017)

Gene signatures from scRNA‐seq accurately quantify mast cells in biopsies in asthma
por: Jiang, Jian, et al.
Publicado: (2020)

The role of HPV-induced epigenetic changes in cervical carcinogenesis (Review)
por: Da Silva, Martha Laysla Ramos, et al.
Publicado: (2021)

Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022)

RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts
por: Vermeulen, Joëlle, et al.
Publicado: (2009)

Design and optimization of an improved qPCR assay for the detection of Histoplasma capsulatum
por: da Silva, Bianca Kelly Neves Izidro, et al.
Publicado: (2023)

EMERGENCY RIGHT HEPATECTOMY FOR PORTA HEPATIS INJURY DURING LAPAROSCOPIC CHOLECYSTECTOMY
por: da FONSECA-NETO, Olival Cirilo Lucena, et al.
Publicado: (2014)

Quantification of siRNA using competitive qPCR
por: Liu, Wei-li, et al.
Publicado: (2009)

The direct and indirect costs of cardiovascular diseases in Brazil
por: de Araújo, Jevuks Matheus, et al.
Publicado: (2022)

Integrity of SRP RNA is ensured by La and the nuclear RNA quality control machinery
por: Leung, Eileen, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_pd1mlui031bmjaeo3730n9plg9