Cargando…
Production of superoxide from Photosystem II in a rice (Oryza sativa L.) mutant lacking PsbS
BACKGROUND: PsbS is a 22-kDa Photosystem (PS) II protein involved in non-photochemical quenching (NPQ) of chlorophyll fluorescence. Rice (Oryza sativa L.) has two PsbS genes, PsbS1 and PsbS2. However, only inactivation of PsbS1, through a knockout (PsbS1-KO) or in RNAi transgenic plants, results in...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219129/ https://www.ncbi.nlm.nih.gov/pubmed/25342550 http://dx.doi.org/10.1186/s12870-014-0242-2 |
_version_ | 1782342536926330880 |
---|---|
author | Zulfugarov, Ismayil S Tovuu, Altanzaya Eu, Young-Jae Dogsom, Bolormaa Poudyal, Roshan Sharma Nath, Krishna Hall, Michael Banerjee, Mainak Yoon, Ung Chan Moon, Yong-Hwan An, Gynheung Jansson, Stefan Lee, Choon-Hwan |
author_facet | Zulfugarov, Ismayil S Tovuu, Altanzaya Eu, Young-Jae Dogsom, Bolormaa Poudyal, Roshan Sharma Nath, Krishna Hall, Michael Banerjee, Mainak Yoon, Ung Chan Moon, Yong-Hwan An, Gynheung Jansson, Stefan Lee, Choon-Hwan |
author_sort | Zulfugarov, Ismayil S |
collection | PubMed |
description | BACKGROUND: PsbS is a 22-kDa Photosystem (PS) II protein involved in non-photochemical quenching (NPQ) of chlorophyll fluorescence. Rice (Oryza sativa L.) has two PsbS genes, PsbS1 and PsbS2. However, only inactivation of PsbS1, through a knockout (PsbS1-KO) or in RNAi transgenic plants, results in plants deficient in qE, the energy-dependent component of NPQ. RESULTS: In studies presented here, under fluctuating high light, growth of young seedlings lacking PsbS is retarded, and PSII in detached leaves of the mutants is more sensitive to photoinhibitory illumination compared with the wild type. Using both histochemical and fluorescent probes, we determined the levels of reactive oxygen species, including singlet oxygen, superoxide, and hydrogen peroxide, in leaves and thylakoids. The PsbS-deficient plants generated more superoxide and hydrogen peroxide in their chloroplasts. PSII complexes isolated from them produced more superoxide compared with the wild type, and PSII-driven superoxide production was higher in the mutants. However, we could not observe such differences either in isolated PSI complexes or through PSI-driven electron transport. Time-course experiments using isolated thylakoids showed that superoxide production was the initial event, and that production of hydrogen peroxide proceeded from that. CONCLUSION: These results indicate that at least some of the photoprotection provided by PsbS and qE is mediated by preventing production of superoxide released from PSII under conditions of excess excitation energy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0242-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4219129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42191292014-11-05 Production of superoxide from Photosystem II in a rice (Oryza sativa L.) mutant lacking PsbS Zulfugarov, Ismayil S Tovuu, Altanzaya Eu, Young-Jae Dogsom, Bolormaa Poudyal, Roshan Sharma Nath, Krishna Hall, Michael Banerjee, Mainak Yoon, Ung Chan Moon, Yong-Hwan An, Gynheung Jansson, Stefan Lee, Choon-Hwan BMC Plant Biol Research Article BACKGROUND: PsbS is a 22-kDa Photosystem (PS) II protein involved in non-photochemical quenching (NPQ) of chlorophyll fluorescence. Rice (Oryza sativa L.) has two PsbS genes, PsbS1 and PsbS2. However, only inactivation of PsbS1, through a knockout (PsbS1-KO) or in RNAi transgenic plants, results in plants deficient in qE, the energy-dependent component of NPQ. RESULTS: In studies presented here, under fluctuating high light, growth of young seedlings lacking PsbS is retarded, and PSII in detached leaves of the mutants is more sensitive to photoinhibitory illumination compared with the wild type. Using both histochemical and fluorescent probes, we determined the levels of reactive oxygen species, including singlet oxygen, superoxide, and hydrogen peroxide, in leaves and thylakoids. The PsbS-deficient plants generated more superoxide and hydrogen peroxide in their chloroplasts. PSII complexes isolated from them produced more superoxide compared with the wild type, and PSII-driven superoxide production was higher in the mutants. However, we could not observe such differences either in isolated PSI complexes or through PSI-driven electron transport. Time-course experiments using isolated thylakoids showed that superoxide production was the initial event, and that production of hydrogen peroxide proceeded from that. CONCLUSION: These results indicate that at least some of the photoprotection provided by PsbS and qE is mediated by preventing production of superoxide released from PSII under conditions of excess excitation energy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0242-2) contains supplementary material, which is available to authorized users. BioMed Central 2014-10-24 /pmc/articles/PMC4219129/ /pubmed/25342550 http://dx.doi.org/10.1186/s12870-014-0242-2 Text en © Zulfugarov et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Zulfugarov, Ismayil S Tovuu, Altanzaya Eu, Young-Jae Dogsom, Bolormaa Poudyal, Roshan Sharma Nath, Krishna Hall, Michael Banerjee, Mainak Yoon, Ung Chan Moon, Yong-Hwan An, Gynheung Jansson, Stefan Lee, Choon-Hwan Production of superoxide from Photosystem II in a rice (Oryza sativa L.) mutant lacking PsbS |
title | Production of superoxide from Photosystem II in a rice (Oryza sativa L.) mutant lacking PsbS |
title_full | Production of superoxide from Photosystem II in a rice (Oryza sativa L.) mutant lacking PsbS |
title_fullStr | Production of superoxide from Photosystem II in a rice (Oryza sativa L.) mutant lacking PsbS |
title_full_unstemmed | Production of superoxide from Photosystem II in a rice (Oryza sativa L.) mutant lacking PsbS |
title_short | Production of superoxide from Photosystem II in a rice (Oryza sativa L.) mutant lacking PsbS |
title_sort | production of superoxide from photosystem ii in a rice (oryza sativa l.) mutant lacking psbs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219129/ https://www.ncbi.nlm.nih.gov/pubmed/25342550 http://dx.doi.org/10.1186/s12870-014-0242-2 |
work_keys_str_mv | AT zulfugarovismayils productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT tovuualtanzaya productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT euyoungjae productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT dogsombolormaa productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT poudyalroshansharma productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT nathkrishna productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT hallmichael productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT banerjeemainak productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT yoonungchan productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT moonyonghwan productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT angynheung productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT janssonstefan productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs AT leechoonhwan productionofsuperoxidefromphotosystemiiinariceoryzasativalmutantlackingpsbs |