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A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens

Here we present a metabolic profiling strategy employing direct infusion Orbitrap mass spectrometry (MS) and gas chromatography-mass spectrometry (GC/MS) for the monitoring of soybean's (Glycine max L.) global metabolism regulation in response to Rhizoctonia solani infection in a time-course. K...

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Autores principales: Aliferis, Konstantinos A., Faubert, Denis, Jabaji, Suha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219818/
https://www.ncbi.nlm.nih.gov/pubmed/25369450
http://dx.doi.org/10.1371/journal.pone.0111930
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author Aliferis, Konstantinos A.
Faubert, Denis
Jabaji, Suha
author_facet Aliferis, Konstantinos A.
Faubert, Denis
Jabaji, Suha
author_sort Aliferis, Konstantinos A.
collection PubMed
description Here we present a metabolic profiling strategy employing direct infusion Orbitrap mass spectrometry (MS) and gas chromatography-mass spectrometry (GC/MS) for the monitoring of soybean's (Glycine max L.) global metabolism regulation in response to Rhizoctonia solani infection in a time-course. Key elements in the approach are the construction of a comprehensive metabolite library for soybean, which accelerates the steps of metabolite identification and biological interpretation of results, and bioinformatics tools for the visualization and analysis of its metabolome. The study of metabolic networks revealed that infection results in the mobilization of carbohydrates, disturbance of the amino acid pool, and activation of isoflavonoid, α-linolenate, and phenylpropanoid biosynthetic pathways of the plant. Components of these pathways include phytoalexins, coumarins, flavonoids, signaling molecules, and hormones, many of which exhibit antioxidant properties and bioactivity helping the plant to counterattack the pathogen's invasion. Unraveling the biochemical mechanism operating during soybean-Rhizoctonia interaction, in addition to its significance towards the understanding of the plant's metabolism regulation under biotic stress, provides valuable insights with potential for applications in biotechnology, crop breeding, and agrochemical and food industries.
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spelling pubmed-42198182014-11-12 A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens Aliferis, Konstantinos A. Faubert, Denis Jabaji, Suha PLoS One Research Article Here we present a metabolic profiling strategy employing direct infusion Orbitrap mass spectrometry (MS) and gas chromatography-mass spectrometry (GC/MS) for the monitoring of soybean's (Glycine max L.) global metabolism regulation in response to Rhizoctonia solani infection in a time-course. Key elements in the approach are the construction of a comprehensive metabolite library for soybean, which accelerates the steps of metabolite identification and biological interpretation of results, and bioinformatics tools for the visualization and analysis of its metabolome. The study of metabolic networks revealed that infection results in the mobilization of carbohydrates, disturbance of the amino acid pool, and activation of isoflavonoid, α-linolenate, and phenylpropanoid biosynthetic pathways of the plant. Components of these pathways include phytoalexins, coumarins, flavonoids, signaling molecules, and hormones, many of which exhibit antioxidant properties and bioactivity helping the plant to counterattack the pathogen's invasion. Unraveling the biochemical mechanism operating during soybean-Rhizoctonia interaction, in addition to its significance towards the understanding of the plant's metabolism regulation under biotic stress, provides valuable insights with potential for applications in biotechnology, crop breeding, and agrochemical and food industries. Public Library of Science 2014-11-04 /pmc/articles/PMC4219818/ /pubmed/25369450 http://dx.doi.org/10.1371/journal.pone.0111930 Text en © 2014 Aliferis et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Aliferis, Konstantinos A.
Faubert, Denis
Jabaji, Suha
A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens
title A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens
title_full A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens
title_fullStr A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens
title_full_unstemmed A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens
title_short A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens
title_sort metabolic profiling strategy for the dissection of plant defense against fungal pathogens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219818/
https://www.ncbi.nlm.nih.gov/pubmed/25369450
http://dx.doi.org/10.1371/journal.pone.0111930
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