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A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens
Here we present a metabolic profiling strategy employing direct infusion Orbitrap mass spectrometry (MS) and gas chromatography-mass spectrometry (GC/MS) for the monitoring of soybean's (Glycine max L.) global metabolism regulation in response to Rhizoctonia solani infection in a time-course. K...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219818/ https://www.ncbi.nlm.nih.gov/pubmed/25369450 http://dx.doi.org/10.1371/journal.pone.0111930 |
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author | Aliferis, Konstantinos A. Faubert, Denis Jabaji, Suha |
author_facet | Aliferis, Konstantinos A. Faubert, Denis Jabaji, Suha |
author_sort | Aliferis, Konstantinos A. |
collection | PubMed |
description | Here we present a metabolic profiling strategy employing direct infusion Orbitrap mass spectrometry (MS) and gas chromatography-mass spectrometry (GC/MS) for the monitoring of soybean's (Glycine max L.) global metabolism regulation in response to Rhizoctonia solani infection in a time-course. Key elements in the approach are the construction of a comprehensive metabolite library for soybean, which accelerates the steps of metabolite identification and biological interpretation of results, and bioinformatics tools for the visualization and analysis of its metabolome. The study of metabolic networks revealed that infection results in the mobilization of carbohydrates, disturbance of the amino acid pool, and activation of isoflavonoid, α-linolenate, and phenylpropanoid biosynthetic pathways of the plant. Components of these pathways include phytoalexins, coumarins, flavonoids, signaling molecules, and hormones, many of which exhibit antioxidant properties and bioactivity helping the plant to counterattack the pathogen's invasion. Unraveling the biochemical mechanism operating during soybean-Rhizoctonia interaction, in addition to its significance towards the understanding of the plant's metabolism regulation under biotic stress, provides valuable insights with potential for applications in biotechnology, crop breeding, and agrochemical and food industries. |
format | Online Article Text |
id | pubmed-4219818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42198182014-11-12 A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens Aliferis, Konstantinos A. Faubert, Denis Jabaji, Suha PLoS One Research Article Here we present a metabolic profiling strategy employing direct infusion Orbitrap mass spectrometry (MS) and gas chromatography-mass spectrometry (GC/MS) for the monitoring of soybean's (Glycine max L.) global metabolism regulation in response to Rhizoctonia solani infection in a time-course. Key elements in the approach are the construction of a comprehensive metabolite library for soybean, which accelerates the steps of metabolite identification and biological interpretation of results, and bioinformatics tools for the visualization and analysis of its metabolome. The study of metabolic networks revealed that infection results in the mobilization of carbohydrates, disturbance of the amino acid pool, and activation of isoflavonoid, α-linolenate, and phenylpropanoid biosynthetic pathways of the plant. Components of these pathways include phytoalexins, coumarins, flavonoids, signaling molecules, and hormones, many of which exhibit antioxidant properties and bioactivity helping the plant to counterattack the pathogen's invasion. Unraveling the biochemical mechanism operating during soybean-Rhizoctonia interaction, in addition to its significance towards the understanding of the plant's metabolism regulation under biotic stress, provides valuable insights with potential for applications in biotechnology, crop breeding, and agrochemical and food industries. Public Library of Science 2014-11-04 /pmc/articles/PMC4219818/ /pubmed/25369450 http://dx.doi.org/10.1371/journal.pone.0111930 Text en © 2014 Aliferis et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Aliferis, Konstantinos A. Faubert, Denis Jabaji, Suha A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens |
title | A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens |
title_full | A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens |
title_fullStr | A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens |
title_full_unstemmed | A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens |
title_short | A Metabolic Profiling Strategy for the Dissection of Plant Defense against Fungal Pathogens |
title_sort | metabolic profiling strategy for the dissection of plant defense against fungal pathogens |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4219818/ https://www.ncbi.nlm.nih.gov/pubmed/25369450 http://dx.doi.org/10.1371/journal.pone.0111930 |
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