Cargando…
(18) F-Fluoride positron emission tomography/computed tomography for noninvasive in vivo quantification of pathophysiological bone metabolism in experimental murine arthritis
INTRODUCTION: Evaluation of disease severity in experimental models of rheumatoid arthritis is inevitably associated with assessment of structural bone damage. A noninvasive imaging technology allowing objective quantification of pathophysiological alterations of bone structure in rodents could subs...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4220085/ https://www.ncbi.nlm.nih.gov/pubmed/25053370 http://dx.doi.org/10.1186/ar4670 |
_version_ | 1782342689940832256 |
---|---|
author | Irmler, Ingo M Gebhardt, Peter Hoffmann, Bianca Opfermann, Thomas Figge, Marc-Thilo Saluz, Hans P Kamradt, Thomas |
author_facet | Irmler, Ingo M Gebhardt, Peter Hoffmann, Bianca Opfermann, Thomas Figge, Marc-Thilo Saluz, Hans P Kamradt, Thomas |
author_sort | Irmler, Ingo M |
collection | PubMed |
description | INTRODUCTION: Evaluation of disease severity in experimental models of rheumatoid arthritis is inevitably associated with assessment of structural bone damage. A noninvasive imaging technology allowing objective quantification of pathophysiological alterations of bone structure in rodents could substantially extend the methods used to date in preclinical arthritis research for staging of autoimmune disease severity or efficacy of therapeutical intervention. Sodium (18) F-fluoride ((18) F-NaF) is a bone-seeking tracer well-suited for molecular imaging. Therefore, we systematically examined the use of (18) F-NaF positron emission tomography/computed tomography (PET/CT) in mice with glucose-6-phosphate isomerase (G6PI)–induced arthritis for quantification of pathological bone metabolism. METHODS: F-fluoride was injected into mice before disease onset and at various time points of progressing experimental arthritis. Radioisotope accumulation in joints in the fore- and hindpaws was analyzed by PET measurements. For validation of bone metabolism quantified by (18) F-fluoride PET, bone surface parameters of high-resolution μCT measurements were used. RESULTS: Before clinical arthritis onset, no distinct accumulation of (18) F-fluoride was detectable in the fore- and hindlimbs of mice immunized with G6PI. In the course of experimental autoimmune disease, (18) F-fluoride bone uptake was increased at sites of enhanced bone metabolism caused by pathophysiological processes of autoimmune disease. Moreover, (18) F-fluoride signaling at different stages of G6PI-induced arthritis was significantly correlated with the degree of bone destruction. CT enabled identification of exact localization of (18) F-fluoride signaling in bone and soft tissue. CONCLUSIONS: The results of this study suggest that small-animal PET/CT using (18) F-fluoride as a tracer is a feasible method for quantitative assessment of pathophysiological bone metabolism in experimental arthritis. Furthermore, the possibility to perform repeated noninvasive measurements in vivo allows longitudinal study of therapeutical intervention monitoring. |
format | Online Article Text |
id | pubmed-4220085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42200852014-11-06 (18) F-Fluoride positron emission tomography/computed tomography for noninvasive in vivo quantification of pathophysiological bone metabolism in experimental murine arthritis Irmler, Ingo M Gebhardt, Peter Hoffmann, Bianca Opfermann, Thomas Figge, Marc-Thilo Saluz, Hans P Kamradt, Thomas Arthritis Res Ther Research Article INTRODUCTION: Evaluation of disease severity in experimental models of rheumatoid arthritis is inevitably associated with assessment of structural bone damage. A noninvasive imaging technology allowing objective quantification of pathophysiological alterations of bone structure in rodents could substantially extend the methods used to date in preclinical arthritis research for staging of autoimmune disease severity or efficacy of therapeutical intervention. Sodium (18) F-fluoride ((18) F-NaF) is a bone-seeking tracer well-suited for molecular imaging. Therefore, we systematically examined the use of (18) F-NaF positron emission tomography/computed tomography (PET/CT) in mice with glucose-6-phosphate isomerase (G6PI)–induced arthritis for quantification of pathological bone metabolism. METHODS: F-fluoride was injected into mice before disease onset and at various time points of progressing experimental arthritis. Radioisotope accumulation in joints in the fore- and hindpaws was analyzed by PET measurements. For validation of bone metabolism quantified by (18) F-fluoride PET, bone surface parameters of high-resolution μCT measurements were used. RESULTS: Before clinical arthritis onset, no distinct accumulation of (18) F-fluoride was detectable in the fore- and hindlimbs of mice immunized with G6PI. In the course of experimental autoimmune disease, (18) F-fluoride bone uptake was increased at sites of enhanced bone metabolism caused by pathophysiological processes of autoimmune disease. Moreover, (18) F-fluoride signaling at different stages of G6PI-induced arthritis was significantly correlated with the degree of bone destruction. CT enabled identification of exact localization of (18) F-fluoride signaling in bone and soft tissue. CONCLUSIONS: The results of this study suggest that small-animal PET/CT using (18) F-fluoride as a tracer is a feasible method for quantitative assessment of pathophysiological bone metabolism in experimental arthritis. Furthermore, the possibility to perform repeated noninvasive measurements in vivo allows longitudinal study of therapeutical intervention monitoring. BioMed Central 2014 2014-07-22 /pmc/articles/PMC4220085/ /pubmed/25053370 http://dx.doi.org/10.1186/ar4670 Text en Copyright © 2014 Irmler et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Irmler, Ingo M Gebhardt, Peter Hoffmann, Bianca Opfermann, Thomas Figge, Marc-Thilo Saluz, Hans P Kamradt, Thomas (18) F-Fluoride positron emission tomography/computed tomography for noninvasive in vivo quantification of pathophysiological bone metabolism in experimental murine arthritis |
title | (18) F-Fluoride positron emission tomography/computed tomography for noninvasive in vivo quantification of pathophysiological bone metabolism in experimental murine arthritis |
title_full | (18) F-Fluoride positron emission tomography/computed tomography for noninvasive in vivo quantification of pathophysiological bone metabolism in experimental murine arthritis |
title_fullStr | (18) F-Fluoride positron emission tomography/computed tomography for noninvasive in vivo quantification of pathophysiological bone metabolism in experimental murine arthritis |
title_full_unstemmed | (18) F-Fluoride positron emission tomography/computed tomography for noninvasive in vivo quantification of pathophysiological bone metabolism in experimental murine arthritis |
title_short | (18) F-Fluoride positron emission tomography/computed tomography for noninvasive in vivo quantification of pathophysiological bone metabolism in experimental murine arthritis |
title_sort | (18) f-fluoride positron emission tomography/computed tomography for noninvasive in vivo quantification of pathophysiological bone metabolism in experimental murine arthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4220085/ https://www.ncbi.nlm.nih.gov/pubmed/25053370 http://dx.doi.org/10.1186/ar4670 |
work_keys_str_mv | AT irmleringom 18ffluoridepositronemissiontomographycomputedtomographyfornoninvasiveinvivoquantificationofpathophysiologicalbonemetabolisminexperimentalmurinearthritis AT gebhardtpeter 18ffluoridepositronemissiontomographycomputedtomographyfornoninvasiveinvivoquantificationofpathophysiologicalbonemetabolisminexperimentalmurinearthritis AT hoffmannbianca 18ffluoridepositronemissiontomographycomputedtomographyfornoninvasiveinvivoquantificationofpathophysiologicalbonemetabolisminexperimentalmurinearthritis AT opfermannthomas 18ffluoridepositronemissiontomographycomputedtomographyfornoninvasiveinvivoquantificationofpathophysiologicalbonemetabolisminexperimentalmurinearthritis AT figgemarcthilo 18ffluoridepositronemissiontomographycomputedtomographyfornoninvasiveinvivoquantificationofpathophysiologicalbonemetabolisminexperimentalmurinearthritis AT saluzhansp 18ffluoridepositronemissiontomographycomputedtomographyfornoninvasiveinvivoquantificationofpathophysiologicalbonemetabolisminexperimentalmurinearthritis AT kamradtthomas 18ffluoridepositronemissiontomographycomputedtomographyfornoninvasiveinvivoquantificationofpathophysiologicalbonemetabolisminexperimentalmurinearthritis |