A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus

Human papillomavirus (HPV) is associated with cervical cancer. In this study, we developed a high-throughput microwell-plate hybrid capture (MPHC) method for epidemiological studies of high-risk HPV (HRHPV). The results with 1238 cervical specimens from female outpatients showed a concordance rate o...

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Autores principales: Wang, Yumei, Liu, Yan, Ding, Yaping, Sun, Nan, Gong, Yafang, Gao, Shangxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2014
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221605/
https://www.ncbi.nlm.nih.gov/pubmed/25091742
http://dx.doi.org/10.1007/s00705-014-2186-0
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author Wang, Yumei
Liu, Yan
Ding, Yaping
Sun, Nan
Gong, Yafang
Gao, Shangxian
author_facet Wang, Yumei
Liu, Yan
Ding, Yaping
Sun, Nan
Gong, Yafang
Gao, Shangxian
author_sort Wang, Yumei
collection PubMed
description Human papillomavirus (HPV) is associated with cervical cancer. In this study, we developed a high-throughput microwell-plate hybrid capture (MPHC) method for epidemiological studies of high-risk HPV (HRHPV). The results with 1238 cervical specimens from female outpatients showed a concordance rate of 94.3 % between the MPHC and Hybrid Capture II assay. The MPHC assay showed an average HRHPV rate of 29.3 % for high-risk populations in populous cities of China. The established MPHC assay could sensitively and specifically detect 13 types of HRHPV and is suitable for large-scale screening, especially in areas where real-time PCR or fluorescence equipment is unavailable. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00705-014-2186-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-42216052014-11-11 A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus Wang, Yumei Liu, Yan Ding, Yaping Sun, Nan Gong, Yafang Gao, Shangxian Arch Virol Brief Report Human papillomavirus (HPV) is associated with cervical cancer. In this study, we developed a high-throughput microwell-plate hybrid capture (MPHC) method for epidemiological studies of high-risk HPV (HRHPV). The results with 1238 cervical specimens from female outpatients showed a concordance rate of 94.3 % between the MPHC and Hybrid Capture II assay. The MPHC assay showed an average HRHPV rate of 29.3 % for high-risk populations in populous cities of China. The established MPHC assay could sensitively and specifically detect 13 types of HRHPV and is suitable for large-scale screening, especially in areas where real-time PCR or fluorescence equipment is unavailable. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00705-014-2186-0) contains supplementary material, which is available to authorized users. Springer Vienna 2014-08-05 2014 /pmc/articles/PMC4221605/ /pubmed/25091742 http://dx.doi.org/10.1007/s00705-014-2186-0 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Brief Report
Wang, Yumei
Liu, Yan
Ding, Yaping
Sun, Nan
Gong, Yafang
Gao, Shangxian
A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus
title A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus
title_full A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus
title_fullStr A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus
title_full_unstemmed A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus
title_short A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus
title_sort pcr-based microwell-plate hybrid capture assay for high-risk human papillomavirus
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221605/
https://www.ncbi.nlm.nih.gov/pubmed/25091742
http://dx.doi.org/10.1007/s00705-014-2186-0
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