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Biological effects of soft denture reline materials on L929 cells in vitro
Soft denture reline materials have been developed to help patients when their oral mucosa is damaged or affected due to ill-fitting dentures or post-implant surgery. Although reports have indicated that these materials leach monomers and other components that do affect their biocompatibility, there...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221910/ https://www.ncbi.nlm.nih.gov/pubmed/25383166 http://dx.doi.org/10.1177/2041731414540911 |
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author | Chaves, Carolina AL Vergani, Carlos E Thomas, Dominique Young, Anne Costa, Carlos AS Salih, Vehid M Machado, Ana L |
author_facet | Chaves, Carolina AL Vergani, Carlos E Thomas, Dominique Young, Anne Costa, Carlos AS Salih, Vehid M Machado, Ana L |
author_sort | Chaves, Carolina AL |
collection | PubMed |
description | Soft denture reline materials have been developed to help patients when their oral mucosa is damaged or affected due to ill-fitting dentures or post-implant surgery. Although reports have indicated that these materials leach monomers and other components that do affect their biocompatibility, there is little information on what cell molecules may be implicated in these material/tissue interactions. The biocompatibility of six soft liners (Ufi Gel P, Sofreliner S, Durabase Soft, Trusoft, Softone and Coe Comfort) was evaluated using a mouse fibroblast cell line, L929. Within 2 h of material disc preparation, each of the materials was exposed by direct contact to L929 cells for periods of 24 and 48 h. The effect of this interaction was assessed by alamarBlue assay (for cell survival). The expression of integrin α(5)β(1) and transforming growth factor β(1) was also assessed using plate assays such as enzyme-linked immunosorbent assay. Trusoft, Softone and Coe Comfort showed significantly reduced cell survival compared with the other soft lining materials at each incubation period. Furthermore, there were significant differences with these same materials in the expression of both integrin α(5)β(1) and transforming growth factor β(1). Soft liner materials may affect cell viability and cellular proteins that have important roles in wound healing and the preservation of cell viability and function in the presence of environmental challenges and stresses. |
format | Online Article Text |
id | pubmed-4221910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-42219102014-11-07 Biological effects of soft denture reline materials on L929 cells in vitro Chaves, Carolina AL Vergani, Carlos E Thomas, Dominique Young, Anne Costa, Carlos AS Salih, Vehid M Machado, Ana L J Tissue Eng Article Soft denture reline materials have been developed to help patients when their oral mucosa is damaged or affected due to ill-fitting dentures or post-implant surgery. Although reports have indicated that these materials leach monomers and other components that do affect their biocompatibility, there is little information on what cell molecules may be implicated in these material/tissue interactions. The biocompatibility of six soft liners (Ufi Gel P, Sofreliner S, Durabase Soft, Trusoft, Softone and Coe Comfort) was evaluated using a mouse fibroblast cell line, L929. Within 2 h of material disc preparation, each of the materials was exposed by direct contact to L929 cells for periods of 24 and 48 h. The effect of this interaction was assessed by alamarBlue assay (for cell survival). The expression of integrin α(5)β(1) and transforming growth factor β(1) was also assessed using plate assays such as enzyme-linked immunosorbent assay. Trusoft, Softone and Coe Comfort showed significantly reduced cell survival compared with the other soft lining materials at each incubation period. Furthermore, there were significant differences with these same materials in the expression of both integrin α(5)β(1) and transforming growth factor β(1). Soft liner materials may affect cell viability and cellular proteins that have important roles in wound healing and the preservation of cell viability and function in the presence of environmental challenges and stresses. SAGE Publications 2014-06-23 /pmc/articles/PMC4221910/ /pubmed/25383166 http://dx.doi.org/10.1177/2041731414540911 Text en © The Author(s) 2014 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (http://www.uk.sagepub.com/aboutus/openaccess.htm). |
spellingShingle | Article Chaves, Carolina AL Vergani, Carlos E Thomas, Dominique Young, Anne Costa, Carlos AS Salih, Vehid M Machado, Ana L Biological effects of soft denture reline materials on L929 cells in vitro |
title | Biological effects of soft denture reline materials on L929 cells in vitro |
title_full | Biological effects of soft denture reline materials on L929 cells in vitro |
title_fullStr | Biological effects of soft denture reline materials on L929 cells in vitro |
title_full_unstemmed | Biological effects of soft denture reline materials on L929 cells in vitro |
title_short | Biological effects of soft denture reline materials on L929 cells in vitro |
title_sort | biological effects of soft denture reline materials on l929 cells in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221910/ https://www.ncbi.nlm.nih.gov/pubmed/25383166 http://dx.doi.org/10.1177/2041731414540911 |
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