Comparison of Structural Architecture of HCV NS3 Genotype 1 versus Pakistani Genotype 3a

This study described the structural characterization of Pakistani HCV NS3 GT3a in parallel with genotypes 1a and 1b NS3. We investigated the role of amino acids and their interaction patterns in different HCV genotypes by crystallographic modeling. Different softwares were used to study the interact...

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Autores principales: Fatima, Kaneez, Azhar, Esam, Mathew, Shilu, Damanhouri, Ghazi, Qadri, Ishtiaq
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221965/
https://www.ncbi.nlm.nih.gov/pubmed/25401105
http://dx.doi.org/10.1155/2014/749254
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author Fatima, Kaneez
Azhar, Esam
Mathew, Shilu
Damanhouri, Ghazi
Qadri, Ishtiaq
author_facet Fatima, Kaneez
Azhar, Esam
Mathew, Shilu
Damanhouri, Ghazi
Qadri, Ishtiaq
author_sort Fatima, Kaneez
collection PubMed
description This study described the structural characterization of Pakistani HCV NS3 GT3a in parallel with genotypes 1a and 1b NS3. We investigated the role of amino acids and their interaction patterns in different HCV genotypes by crystallographic modeling. Different softwares were used to study the interaction pattern, for example, CLCBIO sequence viewer, MODELLER, NMRCLUST, ERRAT score, and MODELLER. Sixty models were produced and clustered into groups and the best model of PK-NCVI/Pk3a NS3 was selected and studied further to check the variability with other HCV NS3 genotypes. This study will help in future to understand the structural architecture of HCV genome variability and to further define the conserved targets for antiviral agents.
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spelling pubmed-42219652014-11-16 Comparison of Structural Architecture of HCV NS3 Genotype 1 versus Pakistani Genotype 3a Fatima, Kaneez Azhar, Esam Mathew, Shilu Damanhouri, Ghazi Qadri, Ishtiaq Biomed Res Int Research Article This study described the structural characterization of Pakistani HCV NS3 GT3a in parallel with genotypes 1a and 1b NS3. We investigated the role of amino acids and their interaction patterns in different HCV genotypes by crystallographic modeling. Different softwares were used to study the interaction pattern, for example, CLCBIO sequence viewer, MODELLER, NMRCLUST, ERRAT score, and MODELLER. Sixty models were produced and clustered into groups and the best model of PK-NCVI/Pk3a NS3 was selected and studied further to check the variability with other HCV NS3 genotypes. This study will help in future to understand the structural architecture of HCV genome variability and to further define the conserved targets for antiviral agents. Hindawi Publishing Corporation 2014 2014-10-21 /pmc/articles/PMC4221965/ /pubmed/25401105 http://dx.doi.org/10.1155/2014/749254 Text en Copyright © 2014 Kaneez Fatima et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fatima, Kaneez
Azhar, Esam
Mathew, Shilu
Damanhouri, Ghazi
Qadri, Ishtiaq
Comparison of Structural Architecture of HCV NS3 Genotype 1 versus Pakistani Genotype 3a
title Comparison of Structural Architecture of HCV NS3 Genotype 1 versus Pakistani Genotype 3a
title_full Comparison of Structural Architecture of HCV NS3 Genotype 1 versus Pakistani Genotype 3a
title_fullStr Comparison of Structural Architecture of HCV NS3 Genotype 1 versus Pakistani Genotype 3a
title_full_unstemmed Comparison of Structural Architecture of HCV NS3 Genotype 1 versus Pakistani Genotype 3a
title_short Comparison of Structural Architecture of HCV NS3 Genotype 1 versus Pakistani Genotype 3a
title_sort comparison of structural architecture of hcv ns3 genotype 1 versus pakistani genotype 3a
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221965/
https://www.ncbi.nlm.nih.gov/pubmed/25401105
http://dx.doi.org/10.1155/2014/749254
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