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Age-specific locomotor response to nicotine in yellow and mottled yellow A(vy)/a mice
BACKGROUND: Most Agouti viable yellow (A(vy)) mice display constitutive expression of agouti protein, which acts as an inverse agonist at the melanocortin receptor 4 (Mc4r), resulting in adult-onset obesity as well as an altered sensitivity to some drugs of abuse. We investigated the influence of ex...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222098/ https://www.ncbi.nlm.nih.gov/pubmed/24289264 http://dx.doi.org/10.1186/1756-0500-6-497 |
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author | Dingman, Marc A Gyekis, Joseph P Whetzel, Courtney A Klein, Laura Cousino Vandenbergh, David J |
author_facet | Dingman, Marc A Gyekis, Joseph P Whetzel, Courtney A Klein, Laura Cousino Vandenbergh, David J |
author_sort | Dingman, Marc A |
collection | PubMed |
description | BACKGROUND: Most Agouti viable yellow (A(vy)) mice display constitutive expression of agouti protein, which acts as an inverse agonist at the melanocortin receptor 4 (Mc4r), resulting in adult-onset obesity as well as an altered sensitivity to some drugs of abuse. We investigated the influence of excessive agouti expression on open-field locomotor response to daily 0.5 mg/kg (-)-freebase nicotine injections in 27 early adolescent and 27 young adult male A(vy)/a and a/a mice, and assessed the effects of nicotine administration (0.5 mg/kg) followed by open-field testing on serum corticosterone levels in a separate group of 25 young adult male A(vy)/a and a/a mice. FINDINGS: Young adult A(vy)/a mice displayed pronounced nicotine-induced hypolocomotion (a 24% reduction in distance traveled) compared to their a/a littermates. Early adolescent A(vy)/a mice did not differ from their a/a littermates or saline-matched controls in locomotion following nicotine administration. Young adult A(vy)/a mice also displayed increased thigmotaxis (a 5% increase in time spent outside the center of the apparatus) on the first day of nicotine administration as compared to saline-matched controls, while a/a mice did not. An increase in serum corticosterone levels 20 minutes after nicotine injection in a separate group of young adult male mice (n = 25) was proportionally similar between A(vy)/a and a/a mice. CONCLUSIONS: Overall, the results suggest an age- and epigenotype- or genotype-specific response to nicotine administration in young adult male A(vy)/a mice. It appears the A(vy)/a locomotor and thigmotaxic responses to acute nicotine administration are not mediated solely by hypothalamic-pituitary-adrenal (HPA) axis stimulation. |
format | Online Article Text |
id | pubmed-4222098 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42220982014-11-10 Age-specific locomotor response to nicotine in yellow and mottled yellow A(vy)/a mice Dingman, Marc A Gyekis, Joseph P Whetzel, Courtney A Klein, Laura Cousino Vandenbergh, David J BMC Res Notes Short Report BACKGROUND: Most Agouti viable yellow (A(vy)) mice display constitutive expression of agouti protein, which acts as an inverse agonist at the melanocortin receptor 4 (Mc4r), resulting in adult-onset obesity as well as an altered sensitivity to some drugs of abuse. We investigated the influence of excessive agouti expression on open-field locomotor response to daily 0.5 mg/kg (-)-freebase nicotine injections in 27 early adolescent and 27 young adult male A(vy)/a and a/a mice, and assessed the effects of nicotine administration (0.5 mg/kg) followed by open-field testing on serum corticosterone levels in a separate group of 25 young adult male A(vy)/a and a/a mice. FINDINGS: Young adult A(vy)/a mice displayed pronounced nicotine-induced hypolocomotion (a 24% reduction in distance traveled) compared to their a/a littermates. Early adolescent A(vy)/a mice did not differ from their a/a littermates or saline-matched controls in locomotion following nicotine administration. Young adult A(vy)/a mice also displayed increased thigmotaxis (a 5% increase in time spent outside the center of the apparatus) on the first day of nicotine administration as compared to saline-matched controls, while a/a mice did not. An increase in serum corticosterone levels 20 minutes after nicotine injection in a separate group of young adult male mice (n = 25) was proportionally similar between A(vy)/a and a/a mice. CONCLUSIONS: Overall, the results suggest an age- and epigenotype- or genotype-specific response to nicotine administration in young adult male A(vy)/a mice. It appears the A(vy)/a locomotor and thigmotaxic responses to acute nicotine administration are not mediated solely by hypothalamic-pituitary-adrenal (HPA) axis stimulation. BioMed Central 2013-12-01 /pmc/articles/PMC4222098/ /pubmed/24289264 http://dx.doi.org/10.1186/1756-0500-6-497 Text en Copyright © 2013 Dingman et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Dingman, Marc A Gyekis, Joseph P Whetzel, Courtney A Klein, Laura Cousino Vandenbergh, David J Age-specific locomotor response to nicotine in yellow and mottled yellow A(vy)/a mice |
title | Age-specific locomotor response to nicotine in yellow and mottled yellow A(vy)/a mice |
title_full | Age-specific locomotor response to nicotine in yellow and mottled yellow A(vy)/a mice |
title_fullStr | Age-specific locomotor response to nicotine in yellow and mottled yellow A(vy)/a mice |
title_full_unstemmed | Age-specific locomotor response to nicotine in yellow and mottled yellow A(vy)/a mice |
title_short | Age-specific locomotor response to nicotine in yellow and mottled yellow A(vy)/a mice |
title_sort | age-specific locomotor response to nicotine in yellow and mottled yellow a(vy)/a mice |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222098/ https://www.ncbi.nlm.nih.gov/pubmed/24289264 http://dx.doi.org/10.1186/1756-0500-6-497 |
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