Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells

Surface-modified maghemite (γ-Fe(2)O(3)) nanoparticles were obtained by using a conventional precipitation method and coated with D-mannose and poly(N,N-dimethylacrylamide). Both the initial and the modified particles were characterized by transmission electron microscopy and dynamic light scatterin...

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Autores principales: Babič, Michal, Horák, Daniel, Lukash, Lyubov L, Ruban, Tetiana A, Kolomiets, Yurii N, Shpylova, Svitlana P, Grypych, Oksana A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Beilstein-Institut 2014
Materias:
Full Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222378/
https://www.ncbi.nlm.nih.gov/pubmed/25383284
http://dx.doi.org/10.3762/bjnano.5.183
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author Babič, Michal
Horák, Daniel
Lukash, Lyubov L
Ruban, Tetiana A
Kolomiets, Yurii N
Shpylova, Svitlana P
Grypych, Oksana A
author_facet Babič, Michal
Horák, Daniel
Lukash, Lyubov L
Ruban, Tetiana A
Kolomiets, Yurii N
Shpylova, Svitlana P
Grypych, Oksana A
author_sort Babič, Michal
collection PubMed
description Surface-modified maghemite (γ-Fe(2)O(3)) nanoparticles were obtained by using a conventional precipitation method and coated with D-mannose and poly(N,N-dimethylacrylamide). Both the initial and the modified particles were characterized by transmission electron microscopy and dynamic light scattering with regard to morphology, particle size and polydispersity. In vitro survival of human stem cells was then investigated by using the methyl thiazolyl tetrazolium (MTT) assay, which showed that D-mannose- and poly(N,N-dimethylacrylamide)-coated γ-Fe(2)O(3) particles exhibit much lower level of cytotoxicity than the non-coated γ-Fe(2)O(3).
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spelling pubmed-42223782014-11-07 Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells Babič, Michal Horák, Daniel Lukash, Lyubov L Ruban, Tetiana A Kolomiets, Yurii N Shpylova, Svitlana P Grypych, Oksana A Beilstein J Nanotechnol Full Research Paper Surface-modified maghemite (γ-Fe(2)O(3)) nanoparticles were obtained by using a conventional precipitation method and coated with D-mannose and poly(N,N-dimethylacrylamide). Both the initial and the modified particles were characterized by transmission electron microscopy and dynamic light scattering with regard to morphology, particle size and polydispersity. In vitro survival of human stem cells was then investigated by using the methyl thiazolyl tetrazolium (MTT) assay, which showed that D-mannose- and poly(N,N-dimethylacrylamide)-coated γ-Fe(2)O(3) particles exhibit much lower level of cytotoxicity than the non-coated γ-Fe(2)O(3). Beilstein-Institut 2014-10-08 /pmc/articles/PMC4222378/ /pubmed/25383284 http://dx.doi.org/10.3762/bjnano.5.183 Text en Copyright © 2014, Babič et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjnano/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (https://www.beilstein-journals.org/bjnano/terms)
spellingShingle Full Research Paper
Babič, Michal
Horák, Daniel
Lukash, Lyubov L
Ruban, Tetiana A
Kolomiets, Yurii N
Shpylova, Svitlana P
Grypych, Oksana A
Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells
title Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells
title_full Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells
title_fullStr Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells
title_full_unstemmed Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells
title_short Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells
title_sort influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222378/
https://www.ncbi.nlm.nih.gov/pubmed/25383284
http://dx.doi.org/10.3762/bjnano.5.183
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