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Collection of Analytes from Microneedle Patches

[Image: see text] Clinical medicine and public health would benefit from simplified acquisition of biological samples from patients that can be easily obtained at point of care, in the field, and by patients themselves. Microneedle patches are designed to serve this need by collecting dermal interst...

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Autores principales: Romanyuk, Andrey V., Zvezdin, Vasiliy N., Samant, Pradnya, Grenader, Mark I., Zemlyanova, Marina, Prausnitz, Mark R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222632/
https://www.ncbi.nlm.nih.gov/pubmed/25367229
http://dx.doi.org/10.1021/ac503823p
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author Romanyuk, Andrey V.
Zvezdin, Vasiliy N.
Samant, Pradnya
Grenader, Mark I.
Zemlyanova, Marina
Prausnitz, Mark R.
author_facet Romanyuk, Andrey V.
Zvezdin, Vasiliy N.
Samant, Pradnya
Grenader, Mark I.
Zemlyanova, Marina
Prausnitz, Mark R.
author_sort Romanyuk, Andrey V.
collection PubMed
description [Image: see text] Clinical medicine and public health would benefit from simplified acquisition of biological samples from patients that can be easily obtained at point of care, in the field, and by patients themselves. Microneedle patches are designed to serve this need by collecting dermal interstitial fluid containing biomarkers without the dangers, pain, or expertise needed to collect blood. This study presents novel methods to collect biomarker analytes from microneedle patches for analysis by integration into conventional analytical laboratory microtubes and microplates. Microneedle patches were made out of cross-linked hydrogel composed of poly(methyl vinyl ether-alt-maleic acid) and poly(ethylene glycol) prepared by micromolding. Microneedle patches were shown to swell with water up to 50-fold in volume, depending on degree of polymer cross-linking, and to collect interstitial fluid from the skin of rats. To collect analytes from microneedle patches, the patches were mounted within the cap of microcentrifuge tubes or formed the top of V-bottom multiwell microplates, and fluid was collected in the bottom of the tubes under gentle centrifugation. In another method, microneedle patches were attached to form the bottom of multiwell microplates, thereby enabling in situ analysis. The simplicity of biological sample acquisition using microneedle patches coupled with the simplicity of analyte collection from microneedles patches integrated into conventional analytical equipment could broaden the reach of future screening, diagnosis, and monitoring of biomarkers in healthcare and environmental/workplace settings.
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spelling pubmed-42226322015-10-17 Collection of Analytes from Microneedle Patches Romanyuk, Andrey V. Zvezdin, Vasiliy N. Samant, Pradnya Grenader, Mark I. Zemlyanova, Marina Prausnitz, Mark R. Anal Chem [Image: see text] Clinical medicine and public health would benefit from simplified acquisition of biological samples from patients that can be easily obtained at point of care, in the field, and by patients themselves. Microneedle patches are designed to serve this need by collecting dermal interstitial fluid containing biomarkers without the dangers, pain, or expertise needed to collect blood. This study presents novel methods to collect biomarker analytes from microneedle patches for analysis by integration into conventional analytical laboratory microtubes and microplates. Microneedle patches were made out of cross-linked hydrogel composed of poly(methyl vinyl ether-alt-maleic acid) and poly(ethylene glycol) prepared by micromolding. Microneedle patches were shown to swell with water up to 50-fold in volume, depending on degree of polymer cross-linking, and to collect interstitial fluid from the skin of rats. To collect analytes from microneedle patches, the patches were mounted within the cap of microcentrifuge tubes or formed the top of V-bottom multiwell microplates, and fluid was collected in the bottom of the tubes under gentle centrifugation. In another method, microneedle patches were attached to form the bottom of multiwell microplates, thereby enabling in situ analysis. The simplicity of biological sample acquisition using microneedle patches coupled with the simplicity of analyte collection from microneedles patches integrated into conventional analytical equipment could broaden the reach of future screening, diagnosis, and monitoring of biomarkers in healthcare and environmental/workplace settings. American Chemical Society 2014-10-17 2014-11-04 /pmc/articles/PMC4222632/ /pubmed/25367229 http://dx.doi.org/10.1021/ac503823p Text en Copyright © 2014 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Romanyuk, Andrey V.
Zvezdin, Vasiliy N.
Samant, Pradnya
Grenader, Mark I.
Zemlyanova, Marina
Prausnitz, Mark R.
Collection of Analytes from Microneedle Patches
title Collection of Analytes from Microneedle Patches
title_full Collection of Analytes from Microneedle Patches
title_fullStr Collection of Analytes from Microneedle Patches
title_full_unstemmed Collection of Analytes from Microneedle Patches
title_short Collection of Analytes from Microneedle Patches
title_sort collection of analytes from microneedle patches
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222632/
https://www.ncbi.nlm.nih.gov/pubmed/25367229
http://dx.doi.org/10.1021/ac503823p
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