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Improved workflows for high throughput library preparation using the transposome-based nextera system

BACKGROUND: The Nextera protocol, which utilises a transposome based approach to create libraries for Illumina sequencing, requires pure DNA template, an accurate assessment of input concentration and a column clean-up that limits its applicability for high-throughput sample preparation. We addresse...

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Detalles Bibliográficos
Autores principales:	Lamble, Sarah, Batty, Elizabeth, Attar, Moustafa, Buck, David, Bowden, Rory, Lunter, Gerton, Crook, Derrick, El-Fahmawi, Bassam, Piazza, Paolo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4222894/ https://www.ncbi.nlm.nih.gov/pubmed/24256843 http://dx.doi.org/10.1186/1472-6750-13-104

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