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NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins
Membrane proteins play key roles in biology. Determination of their structure in a membrane environment, however, is highly challenging. To address this challenge, we developed an approach that couples hydrogen/deuterium exchange of membrane proteins to rapid unfolding and detection by solution-stat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4223039/ https://www.ncbi.nlm.nih.gov/pubmed/25375235 http://dx.doi.org/10.1371/journal.pone.0112374 |
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author | Yao, Xuejun Dürr, Ulrich H. N. Gattin, Zrinka Laukat, Yvonne Narayanan, Rhagavendran L. Brückner, Ann-Kathrin Meisinger, Chris Lange, Adam Becker, Stefan Zweckstetter, Markus |
author_facet | Yao, Xuejun Dürr, Ulrich H. N. Gattin, Zrinka Laukat, Yvonne Narayanan, Rhagavendran L. Brückner, Ann-Kathrin Meisinger, Chris Lange, Adam Becker, Stefan Zweckstetter, Markus |
author_sort | Yao, Xuejun |
collection | PubMed |
description | Membrane proteins play key roles in biology. Determination of their structure in a membrane environment, however, is highly challenging. To address this challenge, we developed an approach that couples hydrogen/deuterium exchange of membrane proteins to rapid unfolding and detection by solution-state NMR spectroscopy. We show that the method allows analysis of the solvent protection of single residues in liposome-embedded proteins such as the 349-residue Tom40, the major protein translocation pore in the outer mitochondrial membrane, which has resisted structural analysis for many years. |
format | Online Article Text |
id | pubmed-4223039 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-42230392014-11-13 NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins Yao, Xuejun Dürr, Ulrich H. N. Gattin, Zrinka Laukat, Yvonne Narayanan, Rhagavendran L. Brückner, Ann-Kathrin Meisinger, Chris Lange, Adam Becker, Stefan Zweckstetter, Markus PLoS One Research Article Membrane proteins play key roles in biology. Determination of their structure in a membrane environment, however, is highly challenging. To address this challenge, we developed an approach that couples hydrogen/deuterium exchange of membrane proteins to rapid unfolding and detection by solution-state NMR spectroscopy. We show that the method allows analysis of the solvent protection of single residues in liposome-embedded proteins such as the 349-residue Tom40, the major protein translocation pore in the outer mitochondrial membrane, which has resisted structural analysis for many years. Public Library of Science 2014-11-06 /pmc/articles/PMC4223039/ /pubmed/25375235 http://dx.doi.org/10.1371/journal.pone.0112374 Text en © 2014 Yao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Yao, Xuejun Dürr, Ulrich H. N. Gattin, Zrinka Laukat, Yvonne Narayanan, Rhagavendran L. Brückner, Ann-Kathrin Meisinger, Chris Lange, Adam Becker, Stefan Zweckstetter, Markus NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins |
title | NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins |
title_full | NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins |
title_fullStr | NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins |
title_full_unstemmed | NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins |
title_short | NMR-Based Detection of Hydrogen/Deuterium Exchange in Liposome-Embedded Membrane Proteins |
title_sort | nmr-based detection of hydrogen/deuterium exchange in liposome-embedded membrane proteins |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4223039/ https://www.ncbi.nlm.nih.gov/pubmed/25375235 http://dx.doi.org/10.1371/journal.pone.0112374 |
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