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Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study

OBJECTIVES: This in vitro study aimed to investigate the ability of Candida albicans (C. albicans) and Enterococcus faecalis (E. faecalis) to penetrate dentinal tubules of instrumented and retreated root canal surface of split human teeth. MATERIALS AND METHODS: Sixty intact extracted human single-r...

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Autores principales: Al-Nazhan, Saad, Al-Sulaiman, Alaa, Al-Rasheed, Fellwa, Alnajjar, Fatimah, Al-Abdulwahab, Bander, Al-Badah, Abdulhakeem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Conservative Dentistry 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4223094/
https://www.ncbi.nlm.nih.gov/pubmed/25383343
http://dx.doi.org/10.5395/rde.2014.39.4.258
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author Al-Nazhan, Saad
Al-Sulaiman, Alaa
Al-Rasheed, Fellwa
Alnajjar, Fatimah
Al-Abdulwahab, Bander
Al-Badah, Abdulhakeem
author_facet Al-Nazhan, Saad
Al-Sulaiman, Alaa
Al-Rasheed, Fellwa
Alnajjar, Fatimah
Al-Abdulwahab, Bander
Al-Badah, Abdulhakeem
author_sort Al-Nazhan, Saad
collection PubMed
description OBJECTIVES: This in vitro study aimed to investigate the ability of Candida albicans (C. albicans) and Enterococcus faecalis (E. faecalis) to penetrate dentinal tubules of instrumented and retreated root canal surface of split human teeth. MATERIALS AND METHODS: Sixty intact extracted human single-rooted teeth were divided into 4 groups, negative control, positive control without canal instrumentation, instrumented, and retreated. Root canals in the instrumented group were enlarged with endodontic instruments, while root canals in the retreated group were enlarged, filled, and then removed the canal filling materials. The teeth were split longitudinally after canal preparation in 3 groups except the negative control group. The teeth were inoculated with both microorganisms separately and in combination. Teeth specimens were examined by scanning electron microscopy (SEM), and the depth of penetration into the dentinal tubules was assessed using the SMILE view software (JEOL Ltd). RESULTS: Penetration of C. albicans and E. faecalis into the dentinal tubules was observed in all 3 groups, although penetration was partially restricted by dentin debris of tubules in the instrumented group and remnants of canal filling materials in the retreated group. In all 3 groups, E. faecalis penetrated deeper into the dentinal tubules by way of cell division than C. albicans which built colonies and penetrated by means of hyphae. CONCLUSIONS: Microorganisms can easily penetrate dentinal tubules of root canals with different appearance based on the microorganism size and status of dentinal tubules.
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spelling pubmed-42230942014-11-07 Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study Al-Nazhan, Saad Al-Sulaiman, Alaa Al-Rasheed, Fellwa Alnajjar, Fatimah Al-Abdulwahab, Bander Al-Badah, Abdulhakeem Restor Dent Endod Research Article OBJECTIVES: This in vitro study aimed to investigate the ability of Candida albicans (C. albicans) and Enterococcus faecalis (E. faecalis) to penetrate dentinal tubules of instrumented and retreated root canal surface of split human teeth. MATERIALS AND METHODS: Sixty intact extracted human single-rooted teeth were divided into 4 groups, negative control, positive control without canal instrumentation, instrumented, and retreated. Root canals in the instrumented group were enlarged with endodontic instruments, while root canals in the retreated group were enlarged, filled, and then removed the canal filling materials. The teeth were split longitudinally after canal preparation in 3 groups except the negative control group. The teeth were inoculated with both microorganisms separately and in combination. Teeth specimens were examined by scanning electron microscopy (SEM), and the depth of penetration into the dentinal tubules was assessed using the SMILE view software (JEOL Ltd). RESULTS: Penetration of C. albicans and E. faecalis into the dentinal tubules was observed in all 3 groups, although penetration was partially restricted by dentin debris of tubules in the instrumented group and remnants of canal filling materials in the retreated group. In all 3 groups, E. faecalis penetrated deeper into the dentinal tubules by way of cell division than C. albicans which built colonies and penetrated by means of hyphae. CONCLUSIONS: Microorganisms can easily penetrate dentinal tubules of root canals with different appearance based on the microorganism size and status of dentinal tubules. The Korean Academy of Conservative Dentistry 2014-11 2014-07-22 /pmc/articles/PMC4223094/ /pubmed/25383343 http://dx.doi.org/10.5395/rde.2014.39.4.258 Text en ©Copyights 2014. The Korean Academy of Conservative Dentistry. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Al-Nazhan, Saad
Al-Sulaiman, Alaa
Al-Rasheed, Fellwa
Alnajjar, Fatimah
Al-Abdulwahab, Bander
Al-Badah, Abdulhakeem
Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study
title Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study
title_full Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study
title_fullStr Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study
title_full_unstemmed Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study
title_short Microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. In vitro SEM study
title_sort microorganism penetration in dentinal tubules of instrumented and retreated root canal walls. in vitro sem study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4223094/
https://www.ncbi.nlm.nih.gov/pubmed/25383343
http://dx.doi.org/10.5395/rde.2014.39.4.258
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