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Hydroxyapatite crystallinity does not affect the repair of critical size bone defects

OBJECTIVE: The physicochemical properties of hydroxyapatite (HA) granules were observed to affect the biological behavior of graft materials. The aim of this work was to analyze the tissue response of two HA granules with different crystallinity and Ca/P ratio in vivo. MATERIAL AND METHODS: The HA g...

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Autores principales: CONZ, Marcio Baltazar, GRANJEIRO, José Mauro, SOARES, Gloria de Almeida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculdade de Odontologia de Bauru da Universidade de São Paulo 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4223784/
https://www.ncbi.nlm.nih.gov/pubmed/21655775
http://dx.doi.org/10.1590/S1678-77572011005000007
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author CONZ, Marcio Baltazar
GRANJEIRO, José Mauro
SOARES, Gloria de Almeida
author_facet CONZ, Marcio Baltazar
GRANJEIRO, José Mauro
SOARES, Gloria de Almeida
author_sort CONZ, Marcio Baltazar
collection PubMed
description OBJECTIVE: The physicochemical properties of hydroxyapatite (HA) granules were observed to affect the biological behavior of graft materials. The aim of this work was to analyze the tissue response of two HA granules with different crystallinity and Ca/P ratio in vivo. MATERIAL AND METHODS: The HA granules were produced in the Biomaterials Laboratory (COPPE/UFRJ). The testing materials were HA granules presenting a Ca/P molar ratio of 1.60 and 28% crystallinity (HA-1), and a Ca/P molar ratio of 1.67 and 70% crystallinity (HA-2). Both HAs were implanted into a critical-size calvaria rat defects. RESULTS: To note, in the control group, the bone defects were filled with blood clot only. Descriptive and histomorphometric analyses after 1, 3, and 6 months postoperatively showed mild inflammatory infiltrate, mainly comprising macrophage-like and multinucleated giant cells, and an increase in the volume density of the fibrous tissues (p<0.05), which was in contrast to the similar volume density of the newly formed bone and biomaterials in relation to the control group. CONCLUSION: Thus, we concluded that HA-1 and HA-2 are biocompatible and non-degradable, and that crystallinity does not affect bone repair of critical size defects.
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spelling pubmed-42237842014-11-12 Hydroxyapatite crystallinity does not affect the repair of critical size bone defects CONZ, Marcio Baltazar GRANJEIRO, José Mauro SOARES, Gloria de Almeida J Appl Oral Sci Original Articles OBJECTIVE: The physicochemical properties of hydroxyapatite (HA) granules were observed to affect the biological behavior of graft materials. The aim of this work was to analyze the tissue response of two HA granules with different crystallinity and Ca/P ratio in vivo. MATERIAL AND METHODS: The HA granules were produced in the Biomaterials Laboratory (COPPE/UFRJ). The testing materials were HA granules presenting a Ca/P molar ratio of 1.60 and 28% crystallinity (HA-1), and a Ca/P molar ratio of 1.67 and 70% crystallinity (HA-2). Both HAs were implanted into a critical-size calvaria rat defects. RESULTS: To note, in the control group, the bone defects were filled with blood clot only. Descriptive and histomorphometric analyses after 1, 3, and 6 months postoperatively showed mild inflammatory infiltrate, mainly comprising macrophage-like and multinucleated giant cells, and an increase in the volume density of the fibrous tissues (p<0.05), which was in contrast to the similar volume density of the newly formed bone and biomaterials in relation to the control group. CONCLUSION: Thus, we concluded that HA-1 and HA-2 are biocompatible and non-degradable, and that crystallinity does not affect bone repair of critical size defects. Faculdade de Odontologia de Bauru da Universidade de São Paulo 2011 /pmc/articles/PMC4223784/ /pubmed/21655775 http://dx.doi.org/10.1590/S1678-77572011005000007 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
CONZ, Marcio Baltazar
GRANJEIRO, José Mauro
SOARES, Gloria de Almeida
Hydroxyapatite crystallinity does not affect the repair of critical size bone defects
title Hydroxyapatite crystallinity does not affect the repair of critical size bone defects
title_full Hydroxyapatite crystallinity does not affect the repair of critical size bone defects
title_fullStr Hydroxyapatite crystallinity does not affect the repair of critical size bone defects
title_full_unstemmed Hydroxyapatite crystallinity does not affect the repair of critical size bone defects
title_short Hydroxyapatite crystallinity does not affect the repair of critical size bone defects
title_sort hydroxyapatite crystallinity does not affect the repair of critical size bone defects
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4223784/
https://www.ncbi.nlm.nih.gov/pubmed/21655775
http://dx.doi.org/10.1590/S1678-77572011005000007
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