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Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes
BACKGROUND: Application of adjuvants with microbial origins is a recently highlighted approach in the vaccinology trials. Archaeosomes are among these microbial compounds with both adjuvant and liposomal activities and features. METHODS: In the present study, recombinant HBsAg encapsulated into Meth...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Avicenna Research Institute
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4224663/ https://www.ncbi.nlm.nih.gov/pubmed/25414786 |
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author | Aghasadeghi, Mohammad Reza Delbaz, Seyed Ali Sadat, Seyed Mehdi Siadat, Seyed Davar Ardestani, Mehdi Shafiee Rahimi, Pooneh Bolhassani, Azam Roudsari, Rouhollah Vahabpour Bahramali, Golnaz Motevalli, Fateme Davari, Mehdi Vakily, Habib Salmani, Ali Sharifat Nobari, Maryam Borhan |
author_facet | Aghasadeghi, Mohammad Reza Delbaz, Seyed Ali Sadat, Seyed Mehdi Siadat, Seyed Davar Ardestani, Mehdi Shafiee Rahimi, Pooneh Bolhassani, Azam Roudsari, Rouhollah Vahabpour Bahramali, Golnaz Motevalli, Fateme Davari, Mehdi Vakily, Habib Salmani, Ali Sharifat Nobari, Maryam Borhan |
author_sort | Aghasadeghi, Mohammad Reza |
collection | PubMed |
description | BACKGROUND: Application of adjuvants with microbial origins is a recently highlighted approach in the vaccinology trials. Archaeosomes are among these microbial compounds with both adjuvant and liposomal activities and features. METHODS: In the present study, recombinant HBsAg encapsulated into Methanobrevibacter smithii (M. smithii) archaeosomes. Balb/c mice immunized with this compound and humoral and cytokine secretion pattern of immunized models analyzed. RESULTS: Frequency of IFN-γ secreting cells in the HBsAg-containing archaeosomes group was significantly higher than HBsAg and HBsAg(+)C/IFA groups (p≤0.05). IgG2a titer in the sera of HBsAg-containing archaeosomes group was also significantly higher than this subclass titer in the other groups (p≤ 0.05). CONCLUSION: Analysis of induced responses revealed the immunopotentiating characteristics of M. smithii archaeosomes in the induction of T-helper 1 responses according to the dominance of IgG2a subtype and IFN-γ secreting splenocytes of immunized mice. |
format | Online Article Text |
id | pubmed-4224663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Avicenna Research Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-42246632014-11-20 Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes Aghasadeghi, Mohammad Reza Delbaz, Seyed Ali Sadat, Seyed Mehdi Siadat, Seyed Davar Ardestani, Mehdi Shafiee Rahimi, Pooneh Bolhassani, Azam Roudsari, Rouhollah Vahabpour Bahramali, Golnaz Motevalli, Fateme Davari, Mehdi Vakily, Habib Salmani, Ali Sharifat Nobari, Maryam Borhan Avicenna J Med Biotechnol Original Article BACKGROUND: Application of adjuvants with microbial origins is a recently highlighted approach in the vaccinology trials. Archaeosomes are among these microbial compounds with both adjuvant and liposomal activities and features. METHODS: In the present study, recombinant HBsAg encapsulated into Methanobrevibacter smithii (M. smithii) archaeosomes. Balb/c mice immunized with this compound and humoral and cytokine secretion pattern of immunized models analyzed. RESULTS: Frequency of IFN-γ secreting cells in the HBsAg-containing archaeosomes group was significantly higher than HBsAg and HBsAg(+)C/IFA groups (p≤0.05). IgG2a titer in the sera of HBsAg-containing archaeosomes group was also significantly higher than this subclass titer in the other groups (p≤ 0.05). CONCLUSION: Analysis of induced responses revealed the immunopotentiating characteristics of M. smithii archaeosomes in the induction of T-helper 1 responses according to the dominance of IgG2a subtype and IFN-γ secreting splenocytes of immunized mice. Avicenna Research Institute 2014 /pmc/articles/PMC4224663/ /pubmed/25414786 Text en Copyright © 2014 Avicenna Research Institute http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Aghasadeghi, Mohammad Reza Delbaz, Seyed Ali Sadat, Seyed Mehdi Siadat, Seyed Davar Ardestani, Mehdi Shafiee Rahimi, Pooneh Bolhassani, Azam Roudsari, Rouhollah Vahabpour Bahramali, Golnaz Motevalli, Fateme Davari, Mehdi Vakily, Habib Salmani, Ali Sharifat Nobari, Maryam Borhan Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes |
title | Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes |
title_full | Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes |
title_fullStr | Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes |
title_full_unstemmed | Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes |
title_short | Induction of Strong and Specific Humoral and T-helper 1 Cellular Responses by HBsAg Entrapped in the Methanobrevibacter smithii Archaeosomes |
title_sort | induction of strong and specific humoral and t-helper 1 cellular responses by hbsag entrapped in the methanobrevibacter smithii archaeosomes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4224663/ https://www.ncbi.nlm.nih.gov/pubmed/25414786 |
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