Applications of RNA interference high-throughput screening technology in cancer biology and virology

RNA interference (RNAi) is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi high-throughput screening (HTS) has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables resea...

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Detalles Bibliográficos
Autores principales: Gao, Shan, Yang, Chen, Jiang, Shan, Xu, Xiao-Ning, Lu, Xin, He, You-Wen, Cheung, Annie, Wang, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Higher Education Press 2014
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4225462/
https://www.ncbi.nlm.nih.gov/pubmed/24952721
http://dx.doi.org/10.1007/s13238-014-0076-6
Descripción
Sumario:RNA interference (RNAi) is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi high-throughput screening (HTS) has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables researchers to identify gene function in a given biological context and will provide considerable novel insight. Here, we review RNAi HTS strategies and applications using case studies in cancer biology and virology.

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