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Rapid Interaction of Helicobacter pylori with Microvilli of the Polar Human Gastric Epithelial Cell Line NCI-N87

Infection with Helicobacter pylori results often in chronic gastritis, gastric ulcers or even gastric tumor development. Little is known about the initial interaction between gastric epithelial cells and H. pylori. The aim of the present study was to analyze the initial host contact to the bacteria....

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Autores principales: Diesing, Anne-Kathrin, Nossol, Constanze, Faber-Zuschratter, Heidi, Zuschratter, Werner, Renner, Lydia, Sokolova, Olga, Naumann, Michael, Rothkötter, Hermann-Josef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4225472/
https://www.ncbi.nlm.nih.gov/pubmed/24136815
http://dx.doi.org/10.1002/ar.22818
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author Diesing, Anne-Kathrin
Nossol, Constanze
Faber-Zuschratter, Heidi
Zuschratter, Werner
Renner, Lydia
Sokolova, Olga
Naumann, Michael
Rothkötter, Hermann-Josef
author_facet Diesing, Anne-Kathrin
Nossol, Constanze
Faber-Zuschratter, Heidi
Zuschratter, Werner
Renner, Lydia
Sokolova, Olga
Naumann, Michael
Rothkötter, Hermann-Josef
author_sort Diesing, Anne-Kathrin
collection PubMed
description Infection with Helicobacter pylori results often in chronic gastritis, gastric ulcers or even gastric tumor development. Little is known about the initial interaction between gastric epithelial cells and H. pylori. The aim of the present study was to analyze the initial host contact to the bacteria. Monolayers of the human gastric epithelial cell line NCI-N87 grown on porous membranes were used and the apical side of the epithelium was exposed to the H. pylori wild-type strain P1 for 1 hr. Many epithelial cells were colonized by bacteria within the period of 60 min. Using scanning electron microscopy we detected that the bacteria were in close contact with the epithelia via microvilli. Further, transmission electron microscopy of the contact sites revealed no difference in the morphology of the microvilli in comparison to those not attached to the bacteria. The present study demonstrates the importance of microvilli on apical epithelial cells during the initial contact of the host by colonizing H. pylori. Anat Rec, 296:1800–1805, 2013. © 2013 The Authors. The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology published by Wiley Periodicals, Inc. on behalf of the American Association of Anatomists.
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spelling pubmed-42254722014-12-15 Rapid Interaction of Helicobacter pylori with Microvilli of the Polar Human Gastric Epithelial Cell Line NCI-N87 Diesing, Anne-Kathrin Nossol, Constanze Faber-Zuschratter, Heidi Zuschratter, Werner Renner, Lydia Sokolova, Olga Naumann, Michael Rothkötter, Hermann-Josef Anat Rec (Hoboken) Cover/Gastrointestinal Biology Infection with Helicobacter pylori results often in chronic gastritis, gastric ulcers or even gastric tumor development. Little is known about the initial interaction between gastric epithelial cells and H. pylori. The aim of the present study was to analyze the initial host contact to the bacteria. Monolayers of the human gastric epithelial cell line NCI-N87 grown on porous membranes were used and the apical side of the epithelium was exposed to the H. pylori wild-type strain P1 for 1 hr. Many epithelial cells were colonized by bacteria within the period of 60 min. Using scanning electron microscopy we detected that the bacteria were in close contact with the epithelia via microvilli. Further, transmission electron microscopy of the contact sites revealed no difference in the morphology of the microvilli in comparison to those not attached to the bacteria. The present study demonstrates the importance of microvilli on apical epithelial cells during the initial contact of the host by colonizing H. pylori. Anat Rec, 296:1800–1805, 2013. © 2013 The Authors. The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology published by Wiley Periodicals, Inc. on behalf of the American Association of Anatomists. Blackwell Publishing Ltd 2013-12 2013-10-17 /pmc/articles/PMC4225472/ /pubmed/24136815 http://dx.doi.org/10.1002/ar.22818 Text en Copyright © The Authors. The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology published by Wiley Periodicals, Inc. on behalf of the American Association of Anatomists http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Cover/Gastrointestinal Biology
Diesing, Anne-Kathrin
Nossol, Constanze
Faber-Zuschratter, Heidi
Zuschratter, Werner
Renner, Lydia
Sokolova, Olga
Naumann, Michael
Rothkötter, Hermann-Josef
Rapid Interaction of Helicobacter pylori with Microvilli of the Polar Human Gastric Epithelial Cell Line NCI-N87
title Rapid Interaction of Helicobacter pylori with Microvilli of the Polar Human Gastric Epithelial Cell Line NCI-N87
title_full Rapid Interaction of Helicobacter pylori with Microvilli of the Polar Human Gastric Epithelial Cell Line NCI-N87
title_fullStr Rapid Interaction of Helicobacter pylori with Microvilli of the Polar Human Gastric Epithelial Cell Line NCI-N87
title_full_unstemmed Rapid Interaction of Helicobacter pylori with Microvilli of the Polar Human Gastric Epithelial Cell Line NCI-N87
title_short Rapid Interaction of Helicobacter pylori with Microvilli of the Polar Human Gastric Epithelial Cell Line NCI-N87
title_sort rapid interaction of helicobacter pylori with microvilli of the polar human gastric epithelial cell line nci-n87
topic Cover/Gastrointestinal Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4225472/
https://www.ncbi.nlm.nih.gov/pubmed/24136815
http://dx.doi.org/10.1002/ar.22818
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