In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil

Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identi...

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Detalles Bibliográficos
Autores principales: Williams, Ryan M., Kulick, Amanda R., Yedlapalli, Srilakshmi, Battistella, Louisa, Hajiran, Cyrus J., Sooter, Letha J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4225842/
https://www.ncbi.nlm.nih.gov/pubmed/25400940
http://dx.doi.org/10.1155/2014/102968
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author Williams, Ryan M.
Kulick, Amanda R.
Yedlapalli, Srilakshmi
Battistella, Louisa
Hajiran, Cyrus J.
Sooter, Letha J.
author_facet Williams, Ryan M.
Kulick, Amanda R.
Yedlapalli, Srilakshmi
Battistella, Louisa
Hajiran, Cyrus J.
Sooter, Letha J.
author_sort Williams, Ryan M.
collection PubMed
description Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (K (d) = 9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device.
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spelling pubmed-42258422014-11-16 In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil Williams, Ryan M. Kulick, Amanda R. Yedlapalli, Srilakshmi Battistella, Louisa Hajiran, Cyrus J. Sooter, Letha J. J Nucleic Acids Research Article Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (K (d) = 9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device. Hindawi Publishing Corporation 2014 2014-10-23 /pmc/articles/PMC4225842/ /pubmed/25400940 http://dx.doi.org/10.1155/2014/102968 Text en Copyright © 2014 Ryan M. Williams et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Williams, Ryan M.
Kulick, Amanda R.
Yedlapalli, Srilakshmi
Battistella, Louisa
Hajiran, Cyrus J.
Sooter, Letha J.
In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil
title In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil
title_full In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil
title_fullStr In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil
title_full_unstemmed In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil
title_short In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil
title_sort in vitro selection of a single-stranded dna molecular recognition element specific for bromacil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4225842/
https://www.ncbi.nlm.nih.gov/pubmed/25400940
http://dx.doi.org/10.1155/2014/102968
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