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Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides

A detailed knowledge of cell wall heterogeneity and complexity is crucial for understanding plant growth and development. One key challenge is to establish links between polysaccharide-rich cell walls and their phenotypic characteristics. It is of particular interest for some plant material, like co...

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Autores principales: Rajasundaram, Dhivyaa, Runavot, Jean-Luc, Guo, Xiaoyuan, Willats, William G. T., Meulewaeter, Frank, Selbig, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226482/
https://www.ncbi.nlm.nih.gov/pubmed/25383868
http://dx.doi.org/10.1371/journal.pone.0112168
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author Rajasundaram, Dhivyaa
Runavot, Jean-Luc
Guo, Xiaoyuan
Willats, William G. T.
Meulewaeter, Frank
Selbig, Joachim
author_facet Rajasundaram, Dhivyaa
Runavot, Jean-Luc
Guo, Xiaoyuan
Willats, William G. T.
Meulewaeter, Frank
Selbig, Joachim
author_sort Rajasundaram, Dhivyaa
collection PubMed
description A detailed knowledge of cell wall heterogeneity and complexity is crucial for understanding plant growth and development. One key challenge is to establish links between polysaccharide-rich cell walls and their phenotypic characteristics. It is of particular interest for some plant material, like cotton fibers, which are of both biological and industrial importance. To this end, we attempted to study cotton fiber characteristics together with glycan arrays using regression based approaches. Taking advantage of the comprehensive microarray polymer profiling technique (CoMPP), 32 cotton lines from different cotton species were studied. The glycan array was generated by sequential extraction of cell wall polysaccharides from mature cotton fibers and screening samples against eleven extensively characterized cell wall probes. Also, phenotypic characteristics of cotton fibers such as length, strength, elongation and micronaire were measured. The relationship between the two datasets was established in an integrative manner using linear regression methods. In the conducted analysis, we demonstrated the usefulness of regression based approaches in establishing a relationship between glycan measurements and phenotypic traits. In addition, the analysis also identified specific polysaccharides which may play a major role during fiber development for the final fiber characteristics. Three different regression methods identified a negative correlation between micronaire and the xyloglucan and homogalacturonan probes. Moreover, homogalacturonan and callose were shown to be significant predictors for fiber length. The role of these polysaccharides was already pointed out in previous cell wall elongation studies. Additional relationships were predicted for fiber strength and elongation which will need further experimental validation.
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spelling pubmed-42264822014-11-13 Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides Rajasundaram, Dhivyaa Runavot, Jean-Luc Guo, Xiaoyuan Willats, William G. T. Meulewaeter, Frank Selbig, Joachim PLoS One Research Article A detailed knowledge of cell wall heterogeneity and complexity is crucial for understanding plant growth and development. One key challenge is to establish links between polysaccharide-rich cell walls and their phenotypic characteristics. It is of particular interest for some plant material, like cotton fibers, which are of both biological and industrial importance. To this end, we attempted to study cotton fiber characteristics together with glycan arrays using regression based approaches. Taking advantage of the comprehensive microarray polymer profiling technique (CoMPP), 32 cotton lines from different cotton species were studied. The glycan array was generated by sequential extraction of cell wall polysaccharides from mature cotton fibers and screening samples against eleven extensively characterized cell wall probes. Also, phenotypic characteristics of cotton fibers such as length, strength, elongation and micronaire were measured. The relationship between the two datasets was established in an integrative manner using linear regression methods. In the conducted analysis, we demonstrated the usefulness of regression based approaches in establishing a relationship between glycan measurements and phenotypic traits. In addition, the analysis also identified specific polysaccharides which may play a major role during fiber development for the final fiber characteristics. Three different regression methods identified a negative correlation between micronaire and the xyloglucan and homogalacturonan probes. Moreover, homogalacturonan and callose were shown to be significant predictors for fiber length. The role of these polysaccharides was already pointed out in previous cell wall elongation studies. Additional relationships were predicted for fiber strength and elongation which will need further experimental validation. Public Library of Science 2014-11-10 /pmc/articles/PMC4226482/ /pubmed/25383868 http://dx.doi.org/10.1371/journal.pone.0112168 Text en © 2014 Rajasundaram et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rajasundaram, Dhivyaa
Runavot, Jean-Luc
Guo, Xiaoyuan
Willats, William G. T.
Meulewaeter, Frank
Selbig, Joachim
Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides
title Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides
title_full Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides
title_fullStr Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides
title_full_unstemmed Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides
title_short Understanding the Relationship between Cotton Fiber Properties and Non-Cellulosic Cell Wall Polysaccharides
title_sort understanding the relationship between cotton fiber properties and non-cellulosic cell wall polysaccharides
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226482/
https://www.ncbi.nlm.nih.gov/pubmed/25383868
http://dx.doi.org/10.1371/journal.pone.0112168
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