Cargando…

A Biomimetic Collagen Derived Peptide Exhibits Anti-Angiogenic Activity in Triple Negative Breast Cancer

We investigated the application of a mimetic 20 amino acid peptide derived from type IV collagen for treatment of breast cancer. We showed that the peptide induced a decrease of proliferation, adhesion, and migration of endothelial and tumor cells in vitro. We also observed an inhibition of triple n...

Descripción completa

Detalles Bibliográficos
Autores principales: Rosca, Elena V., Penet, Marie-France, Mori, Noriko, Koskimaki, Jacob E., Lee, Esak, Pandey, Niranjan B., Bhujwalla, Zaver M., Popel, Aleksander S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226498/
https://www.ncbi.nlm.nih.gov/pubmed/25384034
http://dx.doi.org/10.1371/journal.pone.0111901
_version_ 1782343630766211072
author Rosca, Elena V.
Penet, Marie-France
Mori, Noriko
Koskimaki, Jacob E.
Lee, Esak
Pandey, Niranjan B.
Bhujwalla, Zaver M.
Popel, Aleksander S.
author_facet Rosca, Elena V.
Penet, Marie-France
Mori, Noriko
Koskimaki, Jacob E.
Lee, Esak
Pandey, Niranjan B.
Bhujwalla, Zaver M.
Popel, Aleksander S.
author_sort Rosca, Elena V.
collection PubMed
description We investigated the application of a mimetic 20 amino acid peptide derived from type IV collagen for treatment of breast cancer. We showed that the peptide induced a decrease of proliferation, adhesion, and migration of endothelial and tumor cells in vitro. We also observed an inhibition of triple negative MDA-MB-231 xenograft growth by 75% relative to control when administered intraperitoneally for 27 days at 10 mg/kg. We monitored in vivo the changes in vascular properties throughout the treatment using MRI and found that the vascular volume and permeability surface area product decreased significantly. The treatment also resulted in an increase of caspase-3 activity and in a reduction of microvascular density. The multiple mode of action of this peptide, i.e., anti-angiogenic, and anti-tumorigenic, makes it a viable candidate as a therapeutic agent as a monotherapy or in combination with other compounds.
format Online
Article
Text
id pubmed-4226498
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-42264982014-11-13 A Biomimetic Collagen Derived Peptide Exhibits Anti-Angiogenic Activity in Triple Negative Breast Cancer Rosca, Elena V. Penet, Marie-France Mori, Noriko Koskimaki, Jacob E. Lee, Esak Pandey, Niranjan B. Bhujwalla, Zaver M. Popel, Aleksander S. PLoS One Research Article We investigated the application of a mimetic 20 amino acid peptide derived from type IV collagen for treatment of breast cancer. We showed that the peptide induced a decrease of proliferation, adhesion, and migration of endothelial and tumor cells in vitro. We also observed an inhibition of triple negative MDA-MB-231 xenograft growth by 75% relative to control when administered intraperitoneally for 27 days at 10 mg/kg. We monitored in vivo the changes in vascular properties throughout the treatment using MRI and found that the vascular volume and permeability surface area product decreased significantly. The treatment also resulted in an increase of caspase-3 activity and in a reduction of microvascular density. The multiple mode of action of this peptide, i.e., anti-angiogenic, and anti-tumorigenic, makes it a viable candidate as a therapeutic agent as a monotherapy or in combination with other compounds. Public Library of Science 2014-11-10 /pmc/articles/PMC4226498/ /pubmed/25384034 http://dx.doi.org/10.1371/journal.pone.0111901 Text en © 2014 Rosca et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Rosca, Elena V.
Penet, Marie-France
Mori, Noriko
Koskimaki, Jacob E.
Lee, Esak
Pandey, Niranjan B.
Bhujwalla, Zaver M.
Popel, Aleksander S.
A Biomimetic Collagen Derived Peptide Exhibits Anti-Angiogenic Activity in Triple Negative Breast Cancer
title A Biomimetic Collagen Derived Peptide Exhibits Anti-Angiogenic Activity in Triple Negative Breast Cancer
title_full A Biomimetic Collagen Derived Peptide Exhibits Anti-Angiogenic Activity in Triple Negative Breast Cancer
title_fullStr A Biomimetic Collagen Derived Peptide Exhibits Anti-Angiogenic Activity in Triple Negative Breast Cancer
title_full_unstemmed A Biomimetic Collagen Derived Peptide Exhibits Anti-Angiogenic Activity in Triple Negative Breast Cancer
title_short A Biomimetic Collagen Derived Peptide Exhibits Anti-Angiogenic Activity in Triple Negative Breast Cancer
title_sort biomimetic collagen derived peptide exhibits anti-angiogenic activity in triple negative breast cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226498/
https://www.ncbi.nlm.nih.gov/pubmed/25384034
http://dx.doi.org/10.1371/journal.pone.0111901
work_keys_str_mv AT roscaelenav abiomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT penetmariefrance abiomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT morinoriko abiomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT koskimakijacobe abiomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT leeesak abiomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT pandeyniranjanb abiomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT bhujwallazaverm abiomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT popelaleksanders abiomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT roscaelenav biomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT penetmariefrance biomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT morinoriko biomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT koskimakijacobe biomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT leeesak biomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT pandeyniranjanb biomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT bhujwallazaverm biomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer
AT popelaleksanders biomimeticcollagenderivedpeptideexhibitsantiangiogenicactivityintriplenegativebreastcancer