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PIWIL2 induces c-Myc expression by interacting with NME2 and regulates c-Myc-mediated tumor cell proliferation

c-Myc serves as a crucial regulator in multiple cellular events. Cumulative evidences demonstrate that anomalous c-Myc overexpression correlates with proliferation, invasion and metastasis in various human tumors. However, the transcriptionally activating mechanisms responsible for c-Myc overexpress...

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Autores principales: Yao, Youlin, Li, Chao, Zhou, Xiaoyan, Zhang, Yu, Lu, Yilu, Chen, Jianhui, Zheng, Xulei, Tao, Dachang, Liu, Yunqiang, Ma, Yongxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226697/
https://www.ncbi.nlm.nih.gov/pubmed/25193865
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author Yao, Youlin
Li, Chao
Zhou, Xiaoyan
Zhang, Yu
Lu, Yilu
Chen, Jianhui
Zheng, Xulei
Tao, Dachang
Liu, Yunqiang
Ma, Yongxin
author_facet Yao, Youlin
Li, Chao
Zhou, Xiaoyan
Zhang, Yu
Lu, Yilu
Chen, Jianhui
Zheng, Xulei
Tao, Dachang
Liu, Yunqiang
Ma, Yongxin
author_sort Yao, Youlin
collection PubMed
description c-Myc serves as a crucial regulator in multiple cellular events. Cumulative evidences demonstrate that anomalous c-Myc overexpression correlates with proliferation, invasion and metastasis in various human tumors. However, the transcriptionally activating mechanisms responsible for c-Myc overexpression are complex and continue to be intangible. Here we showed that Piwi-Like RNA-Mediated Gene Silencing 2 (PIWIL2) can upregulate c-Myc via binding with NME/NM23 nucleoside diphosphate kinase 2 (NME2). PIWIL2 promotes c-Myc transcription by interacting with and facilitating NME2 to bind to G4-motif region within c-Myc promoter. Interestingly, in a c-Myc-mediated manner, PIWIL2 upregulates RhoA, which in turn induces filamentary F-actin. Deficiency of PIWIL2 results in obstacle for c-Myc expression, cell cycle progress and cell proliferation. Taken together, our present work demonstrates that PIWIL2 modulates tumor cell proliferation and F-actin filaments via promoting c-Myc expression.
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spelling pubmed-42266972014-11-17 PIWIL2 induces c-Myc expression by interacting with NME2 and regulates c-Myc-mediated tumor cell proliferation Yao, Youlin Li, Chao Zhou, Xiaoyan Zhang, Yu Lu, Yilu Chen, Jianhui Zheng, Xulei Tao, Dachang Liu, Yunqiang Ma, Yongxin Oncotarget Research Paper c-Myc serves as a crucial regulator in multiple cellular events. Cumulative evidences demonstrate that anomalous c-Myc overexpression correlates with proliferation, invasion and metastasis in various human tumors. However, the transcriptionally activating mechanisms responsible for c-Myc overexpression are complex and continue to be intangible. Here we showed that Piwi-Like RNA-Mediated Gene Silencing 2 (PIWIL2) can upregulate c-Myc via binding with NME/NM23 nucleoside diphosphate kinase 2 (NME2). PIWIL2 promotes c-Myc transcription by interacting with and facilitating NME2 to bind to G4-motif region within c-Myc promoter. Interestingly, in a c-Myc-mediated manner, PIWIL2 upregulates RhoA, which in turn induces filamentary F-actin. Deficiency of PIWIL2 results in obstacle for c-Myc expression, cell cycle progress and cell proliferation. Taken together, our present work demonstrates that PIWIL2 modulates tumor cell proliferation and F-actin filaments via promoting c-Myc expression. Impact Journals LLC 2014-08-08 /pmc/articles/PMC4226697/ /pubmed/25193865 Text en Copyright: © 2014 Yao et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Yao, Youlin
Li, Chao
Zhou, Xiaoyan
Zhang, Yu
Lu, Yilu
Chen, Jianhui
Zheng, Xulei
Tao, Dachang
Liu, Yunqiang
Ma, Yongxin
PIWIL2 induces c-Myc expression by interacting with NME2 and regulates c-Myc-mediated tumor cell proliferation
title PIWIL2 induces c-Myc expression by interacting with NME2 and regulates c-Myc-mediated tumor cell proliferation
title_full PIWIL2 induces c-Myc expression by interacting with NME2 and regulates c-Myc-mediated tumor cell proliferation
title_fullStr PIWIL2 induces c-Myc expression by interacting with NME2 and regulates c-Myc-mediated tumor cell proliferation
title_full_unstemmed PIWIL2 induces c-Myc expression by interacting with NME2 and regulates c-Myc-mediated tumor cell proliferation
title_short PIWIL2 induces c-Myc expression by interacting with NME2 and regulates c-Myc-mediated tumor cell proliferation
title_sort piwil2 induces c-myc expression by interacting with nme2 and regulates c-myc-mediated tumor cell proliferation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226697/
https://www.ncbi.nlm.nih.gov/pubmed/25193865
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