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Cytotoxic potential of Anisochilus carnosus (L.f.) wall and estimation of luteolin content by HPLC
BACKGROUND: Anisochilus carnosus (L.f.) wall (Lamiaceae), an annual herb which grows at high altitude is used extensively in folk medicine for the treatment of ailments such as gastric ulcer and skin diseases. The aim of our study was to evaluate the anticancer activity of different extracts of the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226858/ https://www.ncbi.nlm.nih.gov/pubmed/25348394 http://dx.doi.org/10.1186/1472-6882-14-421 |
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author | Bhagat, Jaykumar Lobo, Richard Kumar, Nimmy Mathew, Jessy Elizabeth Pai, Aravinda |
author_facet | Bhagat, Jaykumar Lobo, Richard Kumar, Nimmy Mathew, Jessy Elizabeth Pai, Aravinda |
author_sort | Bhagat, Jaykumar |
collection | PubMed |
description | BACKGROUND: Anisochilus carnosus (L.f.) wall (Lamiaceae), an annual herb which grows at high altitude is used extensively in folk medicine for the treatment of ailments such as gastric ulcer and skin diseases. The aim of our study was to evaluate the anticancer activity of different extracts of the leaves of A.carnosus. An attempt was also made to estimate the luteolin content in different extracts of Anisochilus carnosus by HPLC (High Performance Liquid Chromatography). METHODS: In the current study, we explored the cytotoxic potential of petroleum ether, ethanolic and aqueous extracts of A.carnosus against breast adenocarcinoma cell line (BT-549), by in vitro MTT and SRB assay. We also detected the luteolin content in different extracts (ethanolic and aqueous) of A.carnosus by using HPLC as a tool of analysis. RESULTS: The results demonstrate that petroleum ether and ethanolic extract of A.carnosus showed potent cytotoxic effect against BT-549 with an IC(50) of 22.5 μg/ml (petroleum ether extract) and 87.24 μg/ml (ethanolic extract), by SRB assay, and 18.35 μg/ml (petroleum ether extract) and 58.64 μg/ml (ethanolic extract), by MTT assay. The aqueous extracts showed less cytotoxic effect with an IC(50) of 211.26 μg/ml (by SRB assay) and 238.91 μg/ml (by MTT assay). HPLC results of luteolin content in various extracts using luteolin as the marker compound indicated the ethanol extract to contain the highest concentration of luteolin (0.372% w/w). The aqueous extract contained lower concentration of luteolin (0.282% w/w). CONCLUSION: Our findings demonstrate that petroleum ether and ethanolic extract of A.carnosus shows promising anticancer activity and has the potential to be developed into a therapeutic option for the treatment of cancer. |
format | Online Article Text |
id | pubmed-4226858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42268582014-11-12 Cytotoxic potential of Anisochilus carnosus (L.f.) wall and estimation of luteolin content by HPLC Bhagat, Jaykumar Lobo, Richard Kumar, Nimmy Mathew, Jessy Elizabeth Pai, Aravinda BMC Complement Altern Med Research Article BACKGROUND: Anisochilus carnosus (L.f.) wall (Lamiaceae), an annual herb which grows at high altitude is used extensively in folk medicine for the treatment of ailments such as gastric ulcer and skin diseases. The aim of our study was to evaluate the anticancer activity of different extracts of the leaves of A.carnosus. An attempt was also made to estimate the luteolin content in different extracts of Anisochilus carnosus by HPLC (High Performance Liquid Chromatography). METHODS: In the current study, we explored the cytotoxic potential of petroleum ether, ethanolic and aqueous extracts of A.carnosus against breast adenocarcinoma cell line (BT-549), by in vitro MTT and SRB assay. We also detected the luteolin content in different extracts (ethanolic and aqueous) of A.carnosus by using HPLC as a tool of analysis. RESULTS: The results demonstrate that petroleum ether and ethanolic extract of A.carnosus showed potent cytotoxic effect against BT-549 with an IC(50) of 22.5 μg/ml (petroleum ether extract) and 87.24 μg/ml (ethanolic extract), by SRB assay, and 18.35 μg/ml (petroleum ether extract) and 58.64 μg/ml (ethanolic extract), by MTT assay. The aqueous extracts showed less cytotoxic effect with an IC(50) of 211.26 μg/ml (by SRB assay) and 238.91 μg/ml (by MTT assay). HPLC results of luteolin content in various extracts using luteolin as the marker compound indicated the ethanol extract to contain the highest concentration of luteolin (0.372% w/w). The aqueous extract contained lower concentration of luteolin (0.282% w/w). CONCLUSION: Our findings demonstrate that petroleum ether and ethanolic extract of A.carnosus shows promising anticancer activity and has the potential to be developed into a therapeutic option for the treatment of cancer. BioMed Central 2014-10-28 /pmc/articles/PMC4226858/ /pubmed/25348394 http://dx.doi.org/10.1186/1472-6882-14-421 Text en © Bhagat et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Bhagat, Jaykumar Lobo, Richard Kumar, Nimmy Mathew, Jessy Elizabeth Pai, Aravinda Cytotoxic potential of Anisochilus carnosus (L.f.) wall and estimation of luteolin content by HPLC |
title | Cytotoxic potential of Anisochilus carnosus (L.f.) wall and estimation of luteolin content by HPLC |
title_full | Cytotoxic potential of Anisochilus carnosus (L.f.) wall and estimation of luteolin content by HPLC |
title_fullStr | Cytotoxic potential of Anisochilus carnosus (L.f.) wall and estimation of luteolin content by HPLC |
title_full_unstemmed | Cytotoxic potential of Anisochilus carnosus (L.f.) wall and estimation of luteolin content by HPLC |
title_short | Cytotoxic potential of Anisochilus carnosus (L.f.) wall and estimation of luteolin content by HPLC |
title_sort | cytotoxic potential of anisochilus carnosus (l.f.) wall and estimation of luteolin content by hplc |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226858/ https://www.ncbi.nlm.nih.gov/pubmed/25348394 http://dx.doi.org/10.1186/1472-6882-14-421 |
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