Cargando…
Simulate_PCR for amplicon prediction and annotation from multiplex, degenerate primers and probes
BACKGROUND: Pairing up primers to amplify desired targets and avoid undesired cross reactions can be a combinatorial challenge. Effective prediction of specificity and inclusivity from multiplexed primers and TaqMan®/Luminex® probes is a critical step in PCR design. RESULTS: Code is described to ide...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2014
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4226945/ https://www.ncbi.nlm.nih.gov/pubmed/25005023 http://dx.doi.org/10.1186/1471-2105-15-237 |