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Heparin Assisted Photochemical Synthesis of Gold Nanoparticles and Their Performance as SERS Substrates

Reactive and pharmaceutical-grade heparins were used as biologically compatible reducing and stabilizing agents to photochemically synthesize colloidal gold nanoparticles. Aggregates and anisotropic shapes were obtained photochemically under UV black-light lamp irradiation (λ = 366 nm). Heparin-func...

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Autores principales: Rodríguez-Torres, Maria del Pilar, Díaz-Torres, Luis Armando, Romero-Servin, Sergio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227271/
https://www.ncbi.nlm.nih.gov/pubmed/25342319
http://dx.doi.org/10.3390/ijms151019239
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author Rodríguez-Torres, Maria del Pilar
Díaz-Torres, Luis Armando
Romero-Servin, Sergio
author_facet Rodríguez-Torres, Maria del Pilar
Díaz-Torres, Luis Armando
Romero-Servin, Sergio
author_sort Rodríguez-Torres, Maria del Pilar
collection PubMed
description Reactive and pharmaceutical-grade heparins were used as biologically compatible reducing and stabilizing agents to photochemically synthesize colloidal gold nanoparticles. Aggregates and anisotropic shapes were obtained photochemically under UV black-light lamp irradiation (λ = 366 nm). Heparin-functionalized gold nanoparticles were characterized by Scanning Electron Microscopy and UV-Vis spectroscopy. The negatively charged colloids were used for the Surface Enhanced Raman Spectroscopy (SERS) analysis of differently charged analytes (dyes). Measurements of pH were taken to inspect how the acidity of the medium affects the colloid-analyte interaction. SERS spectra were taken by mixing the dyes and the colloidal solutions without further functionalization or addition of any aggregating agent.
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spelling pubmed-42272712014-11-12 Heparin Assisted Photochemical Synthesis of Gold Nanoparticles and Their Performance as SERS Substrates Rodríguez-Torres, Maria del Pilar Díaz-Torres, Luis Armando Romero-Servin, Sergio Int J Mol Sci Article Reactive and pharmaceutical-grade heparins were used as biologically compatible reducing and stabilizing agents to photochemically synthesize colloidal gold nanoparticles. Aggregates and anisotropic shapes were obtained photochemically under UV black-light lamp irradiation (λ = 366 nm). Heparin-functionalized gold nanoparticles were characterized by Scanning Electron Microscopy and UV-Vis spectroscopy. The negatively charged colloids were used for the Surface Enhanced Raman Spectroscopy (SERS) analysis of differently charged analytes (dyes). Measurements of pH were taken to inspect how the acidity of the medium affects the colloid-analyte interaction. SERS spectra were taken by mixing the dyes and the colloidal solutions without further functionalization or addition of any aggregating agent. MDPI 2014-10-23 /pmc/articles/PMC4227271/ /pubmed/25342319 http://dx.doi.org/10.3390/ijms151019239 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodríguez-Torres, Maria del Pilar
Díaz-Torres, Luis Armando
Romero-Servin, Sergio
Heparin Assisted Photochemical Synthesis of Gold Nanoparticles and Their Performance as SERS Substrates
title Heparin Assisted Photochemical Synthesis of Gold Nanoparticles and Their Performance as SERS Substrates
title_full Heparin Assisted Photochemical Synthesis of Gold Nanoparticles and Their Performance as SERS Substrates
title_fullStr Heparin Assisted Photochemical Synthesis of Gold Nanoparticles and Their Performance as SERS Substrates
title_full_unstemmed Heparin Assisted Photochemical Synthesis of Gold Nanoparticles and Their Performance as SERS Substrates
title_short Heparin Assisted Photochemical Synthesis of Gold Nanoparticles and Their Performance as SERS Substrates
title_sort heparin assisted photochemical synthesis of gold nanoparticles and their performance as sers substrates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227271/
https://www.ncbi.nlm.nih.gov/pubmed/25342319
http://dx.doi.org/10.3390/ijms151019239
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