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CCR7 Regulates Cell Migration and Invasion through JAK2/STAT3 in Metastatic Squamous Cell Carcinoma of the Head and Neck

Squamous cell carcinoma of the head and neck (SCCHN) frequently involves metastasis at diagnosis. Our previous research has demonstrated that CCR7 plays a key role in regulating SCCHN metastasis, and this process involves several molecules, such as PI3K/cdc42, pyk2, and Src. In this study, the goals...

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Autores principales: Liu, Fa-Yu, Safdar, Jawad, Li, Zhen-Ning, Fang, Qi-Gen, Zhang, Xu, Xu, Zhong-Fei, Sun, Chang-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227331/
https://www.ncbi.nlm.nih.gov/pubmed/25405202
http://dx.doi.org/10.1155/2014/415375
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author Liu, Fa-Yu
Safdar, Jawad
Li, Zhen-Ning
Fang, Qi-Gen
Zhang, Xu
Xu, Zhong-Fei
Sun, Chang-Fu
author_facet Liu, Fa-Yu
Safdar, Jawad
Li, Zhen-Ning
Fang, Qi-Gen
Zhang, Xu
Xu, Zhong-Fei
Sun, Chang-Fu
author_sort Liu, Fa-Yu
collection PubMed
description Squamous cell carcinoma of the head and neck (SCCHN) frequently involves metastasis at diagnosis. Our previous research has demonstrated that CCR7 plays a key role in regulating SCCHN metastasis, and this process involves several molecules, such as PI3K/cdc42, pyk2, and Src. In this study, the goals are to identify whether JAK2/STAT3 also participates in CCR7's signal network, its relationship with other signal pathways, and its role in SCCHN cell invasion and migration. The results showed that stimulation of CCL19 could induce JAK2/STAT3 phosphorylation, which can be blocked by Src and pyk2 inhibitors. After activation, STAT3 was able to promote low expression of E-cadherin and had no effect on vimentin. This JAk2/STAT3 pathway not only mediated CCR7-induced cell migration but also mediated invasion speed. The immunohistochemistry results also showed that the phosphorylation of STAT3 was correlated with CCR7 expression in SCCHN, and CCR7 and STAT3 phosphorylation were all associated with lymph node metastasis. In conclusion, JAk2/STAT3 plays a key role in CCR7 regulating SCCHN metastasis.
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spelling pubmed-42273312014-11-17 CCR7 Regulates Cell Migration and Invasion through JAK2/STAT3 in Metastatic Squamous Cell Carcinoma of the Head and Neck Liu, Fa-Yu Safdar, Jawad Li, Zhen-Ning Fang, Qi-Gen Zhang, Xu Xu, Zhong-Fei Sun, Chang-Fu Biomed Res Int Research Article Squamous cell carcinoma of the head and neck (SCCHN) frequently involves metastasis at diagnosis. Our previous research has demonstrated that CCR7 plays a key role in regulating SCCHN metastasis, and this process involves several molecules, such as PI3K/cdc42, pyk2, and Src. In this study, the goals are to identify whether JAK2/STAT3 also participates in CCR7's signal network, its relationship with other signal pathways, and its role in SCCHN cell invasion and migration. The results showed that stimulation of CCL19 could induce JAK2/STAT3 phosphorylation, which can be blocked by Src and pyk2 inhibitors. After activation, STAT3 was able to promote low expression of E-cadherin and had no effect on vimentin. This JAk2/STAT3 pathway not only mediated CCR7-induced cell migration but also mediated invasion speed. The immunohistochemistry results also showed that the phosphorylation of STAT3 was correlated with CCR7 expression in SCCHN, and CCR7 and STAT3 phosphorylation were all associated with lymph node metastasis. In conclusion, JAk2/STAT3 plays a key role in CCR7 regulating SCCHN metastasis. Hindawi Publishing Corporation 2014 2014-10-27 /pmc/articles/PMC4227331/ /pubmed/25405202 http://dx.doi.org/10.1155/2014/415375 Text en Copyright © 2014 Fa-Yu Liu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Fa-Yu
Safdar, Jawad
Li, Zhen-Ning
Fang, Qi-Gen
Zhang, Xu
Xu, Zhong-Fei
Sun, Chang-Fu
CCR7 Regulates Cell Migration and Invasion through JAK2/STAT3 in Metastatic Squamous Cell Carcinoma of the Head and Neck
title CCR7 Regulates Cell Migration and Invasion through JAK2/STAT3 in Metastatic Squamous Cell Carcinoma of the Head and Neck
title_full CCR7 Regulates Cell Migration and Invasion through JAK2/STAT3 in Metastatic Squamous Cell Carcinoma of the Head and Neck
title_fullStr CCR7 Regulates Cell Migration and Invasion through JAK2/STAT3 in Metastatic Squamous Cell Carcinoma of the Head and Neck
title_full_unstemmed CCR7 Regulates Cell Migration and Invasion through JAK2/STAT3 in Metastatic Squamous Cell Carcinoma of the Head and Neck
title_short CCR7 Regulates Cell Migration and Invasion through JAK2/STAT3 in Metastatic Squamous Cell Carcinoma of the Head and Neck
title_sort ccr7 regulates cell migration and invasion through jak2/stat3 in metastatic squamous cell carcinoma of the head and neck
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227331/
https://www.ncbi.nlm.nih.gov/pubmed/25405202
http://dx.doi.org/10.1155/2014/415375
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