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Long-Range Distance Measurements in Proteins at Physiological Temperatures Using Saturation Recovery EPR Spectroscopy
[Image: see text] Site-directed spin labeling in combination with EPR is a powerful method for providing distances on the nm scale in biological systems. The most popular strategy, double electron–electron resonance (DEER), is carried out at cryogenic temperatures (50–80 K) to increase the short spi...
Autores principales: | Yang, Zhongyu, Jiménez-Osés, Gonzalo, López, Carlos J., Bridges, Michael D., Houk, K. N., Hubbell, Wayne L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2014
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227719/ https://www.ncbi.nlm.nih.gov/pubmed/25290172 http://dx.doi.org/10.1021/ja5083206 |
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