Cargando…

Crystal structure, mutational analysis and RNA-dependent ATPase activity of the yeast DEAD-box pre-mRNA splicing factor Prp28

Yeast Prp28 is a DEAD-box pre-mRNA splicing factor implicated in displacing U1 snRNP from the 5′ splice site. Here we report that the 588-aa Prp28 protein consists of a trypsin-sensitive 126-aa N-terminal segment (of which aa 1–89 are dispensable for Prp28 function in vivo) fused to a trypsin-resist...

Descripción completa

Detalles Bibliográficos
Autores principales:	Jacewicz, Agata, Schwer, Beate, Smith, Paul, Shuman, Stewart
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Structural Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227776/ https://www.ncbi.nlm.nih.gov/pubmed/25303995 http://dx.doi.org/10.1093/nar/gku930

Ejemplares similares

Structural basis for recognition of intron branchpoint RNA by yeast Msl5 and selective effects of interfacial mutations on splicing of yeast pre-mRNAs
por: Jacewicz, Agata, et al.
Publicado: (2015)

Structure-function analysis of the Yhc1 subunit of yeast U1 snRNP and genetic interactions of Yhc1 with Mud2, Nam8, Mud1, Tgs1, U1 snRNA, SmD3 and Prp28
por: Schwer, Beate, et al.
Publicado: (2014)

An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8
por: Price, Argenta M., et al.
Publicado: (2014)

Splicing proofreading at 5′ splice sites by ATPase Prp28p
por: Yang, Fei, et al.
Publicado: (2013)

Structural basis for RNA translocation by DEAH-box ATPases
por: Hamann, Florian, et al.
Publicado: (2019)

Structure of mycobacterial 3′-to-5′ RNA:DNA helicase Lhr bound to a ssDNA tracking strand highlights distinctive features of a novel family of bacterial helicases
por: Ejaz, Anam, et al.
Publicado: (2018)

Defining essential elements and genetic interactions of the yeast Lsm2–8 ring and demonstration that essentiality of Lsm2–8 is bypassed via overexpression of U6 snRNA or the U6 snRNP subunit Prp24
por: Roth, Allen J., et al.
Publicado: (2018)

Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28
por: Möhlmann, Sina, et al.
Publicado: (2014)

Structure–function analysis of the kinase-CPD domain of yeast tRNA ligase (Trl1) and requirements for complementation of tRNA splicing by a plant Trl1 homolog
por: Wang, Li Kai, et al.
Publicado: (2006)

Transcriptional profiling of fission yeast RNA polymerase II CTD mutants
por: Garg, Angad, et al.
Publicado: (2021)

Mechanistic insights into the manganese-dependent phosphodiesterase activity of yeast Dbr1 with bis-p-nitrophenylphosphate and branched RNA substrates
por: Schwer, Beate, et al.
Publicado: (2016)

A Snu114–GTP–Prp8 module forms a relay station for efficient splicing in yeast
por: Jia, Junqiao, et al.
Publicado: (2020)

A conserved mechanism of DEAD-box ATPase activation by nucleoporins and IP(6) in mRNA export
por: Montpetit, Ben, et al.
Publicado: (2011)

RNA polymerase II CTD phospho-sites Ser5 and Ser7 govern phosphate homeostasis in fission yeast
por: Schwer, Beate, et al.
Publicado: (2015)

An essential role for trimethylguanosine RNA caps in Saccharomyces cerevisiae meiosis and their requirement for splicing of SAE3 and PCH2 meiotic pre-mRNAs
por: Qiu, Zhicheng R., et al.
Publicado: (2011)

Termination of pre-mRNA splicing requires that the ATPase and RNA unwindase Prp43p acts on the catalytic snRNA U6
por: Toroney, Rebecca, et al.
Publicado: (2019)

Yeast Prp2 liberates the 5′ splice site and the branch site adenosine for catalysis of pre-mRNA splicing
por: Bao, Penghui, et al.
Publicado: (2017)

Structure–function analysis of yeast RNA debranching enzyme (Dbr1), a manganese-dependent phosphodiesterase
por: Khalid, M. Fahad, et al.
Publicado: (2005)

Fission yeast RNA triphosphatase reads an Spt5 CTD code
por: Doamekpor, Selom K., et al.
Publicado: (2015)

Inactivation of fission yeast Erh1 de-represses pho1 expression: evidence that Erh1 is a negative regulator of prt lncRNA termination
por: Schwer, Beate, et al.
Publicado: (2020)

Structure-function analysis of the 5′ end of yeast U1 snRNA highlights genetic interactions with the Msl5•Mud2 branchpoint-binding complex and other spliceosome assembly factors
por: Schwer, Beate, et al.
Publicado: (2013)

Poly(A) site choice and Pol2 CTD Serine-5 status govern lncRNA control of phosphate-responsive tgp1 gene expression in fission yeast
por: Sanchez, Ana M., et al.
Publicado: (2018)

A genetic screen for suppressors of hyper-repression of the fission yeast PHO regulon by Pol2 CTD mutation T4A implicates inositol 1-pyrophosphates as agonists of precocious lncRNA transcription termination
por: Garg, Angad, et al.
Publicado: (2020)

How an mRNA capping enzyme reads distinct RNA polymerase II and Spt5 CTD phosphorylation codes
por: Doamekpor, Selom K., et al.
Publicado: (2014)

Sinefungin resistance of Saccharomyces cerevisiae arising from sam3 mutations that inactivate the AdoMet transporter or from increased expression of AdoMet synthase plus mRNA cap guanine-N7 methyltransferase
por: Zheng, Sushuang, et al.
Publicado: (2007)

Activity and substrate specificity of Candida, Aspergillus, and Coccidioides Tpt1: essential tRNA splicing enzymes and potential antifungal targets
por: Dantuluri, Swathi, et al.
Publicado: (2021)

Genetic and structural analysis of the essential fission yeast RNA polymerase II CTD phosphatase Fcp1
por: Schwer, Beate, et al.
Publicado: (2015)

Transcription of lncRNA prt, clustered prt RNA sites for Mmi1 binding, and RNA polymerase II CTD phospho-sites govern the repression of pho1 gene expression under phosphate-replete conditions in fission yeast
por: Chatterjee, Debashree, et al.
Publicado: (2016)

Determinants of Nam8-dependent splicing of meiotic pre-mRNAs
por: Qiu, Zhicheng R., et al.
Publicado: (2011)

Crystal structure of the spliceosomal DEAH-box ATPase Prp2
por: Schmitt, Andreas, et al.
Publicado: (2018)

Structure–function analysis and genetic interactions of the yeast branchpoint binding protein Msl5
por: Chang, Jonathan, et al.
Publicado: (2012)

Characterization of the tRNA ligases of pathogenic fungi Aspergillus fumigatus and Coccidioides immitis
por: Remus, Barbara S., et al.
Publicado: (2016)

Structure and mechanism of E. coli RNA 2′,3′-cyclic phosphodiesterase
por: Remus, Barbara S., et al.
Publicado: (2014)

Structures of RNA ligase RtcB in complexes with divalent cations and GTP
por: Jacewicz, Agata, et al.
Publicado: (2022)

Genetic interactions and transcriptomics implicate fission yeast CTD prolyl isomerase Pin1 as an agent of RNA 3′ processing and transcription termination that functions via its effects on CTD phosphatase Ssu72
por: Sanchez, Ana M, et al.
Publicado: (2020)

Dynamics of the DEAD-box ATPase Prp5 RecA-like domains provide a conformational switch during spliceosome assembly
por: Beier, David H, et al.
Publicado: (2019)

The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome
por: Wlodaver, Alissa M., et al.
Publicado: (2014)

A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop
por: Martin-Tumasz, Stephen, et al.
Publicado: (2011)

Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2
por: Hamann, Florian, et al.
Publicado: (2020)

Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis
por: Garg, Angad, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_aggn1q063skf993acgnf550g17