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Chloroform-Assisted Phenol Extraction Improving Proteome Profiling of Maize Embryos through Selective Depletion of High-Abundance Storage Proteins

The presence of abundant storage proteins in plant embryos greatly impedes seed proteomics analysis. Vicilin (or globulin-1) is the most abundant storage protein in maize embryo. There is a need to deplete the vicilins from maize embryo extracts for enhanced proteomics analysis. We here reported a c...

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Detalles Bibliográficos
Autores principales: Xiong, Erhui, Wu, Xiaolin, Yang, Le, Gong, Fangping, Tai, Fuju, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227863/
https://www.ncbi.nlm.nih.gov/pubmed/25386674
http://dx.doi.org/10.1371/journal.pone.0112724