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CANDLES, an assay for monitoring GPCR induced cAMP generation in cell cultures

BACKGROUND: G protein-coupled receptors (GPCRs) represent a physiologically and pharmacologically important family of receptors that upon coupling to Gα(S) stimulate cAMP production catalyzed by adenylyl cyclase. Thus, developing assays to monitor cAMP production is crucial to screen for ligands in...

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Detalles Bibliográficos
Autores principales:	Trehan, Ashutosh, Rotgers, Emmi, Coffey, Eleanor T, Huhtaniemi, Ilpo, Rivero-Müller, Adolfo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228090/ https://www.ncbi.nlm.nih.gov/pubmed/25366423 http://dx.doi.org/10.1186/s12964-014-0070-x

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