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Detrimental effect of the 6 His C-terminal tag on YedY enzymatic activity and influence of the TAT signal sequence on YedY synthesis
BACKGROUND: YedY, a molybdoenzyme belonging to the sulfite oxidase family, is found in most Gram-negative bacteria. It contains a twin-arginine signal sequence that is cleaved after its translocation into the periplasm. Despite a weak reductase activity with substrates such as dimethyl sulfoxide or...
Autores principales: | Sabaty, Monique, Grosse, Sandrine, Adryanczyk, Geraldine, Boiry, Séverine, Biaso, Frédéric, Arnoux, Pascal, Pignol, David |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228395/ https://www.ncbi.nlm.nih.gov/pubmed/24180491 http://dx.doi.org/10.1186/1471-2091-14-28 |
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