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Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer
BACKGROUND: Paxillin (PXN) has been found to be aberrantly regulated in various malignancies and involved in tumor growth and invasion. The clinicopathological and prognostic significance of PXN in gastric cancer is still unclear. METHODS: The expression of PXN was determined in paired gastric cance...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228400/ https://www.ncbi.nlm.nih.gov/pubmed/24180516 http://dx.doi.org/10.1186/1479-5876-11-277 |
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author | Chen, Dong-liang Wang, Zhi-qiang Ren, Chao Zeng, Zhao-lei Wang, De-shen Luo, Hui-yan Wang, Feng Qiu, Miao-zhen Bai, Long Zhang, Dong-sheng Wang, Feng-hua Li, Yu-hong Xu, Rui-hua |
author_facet | Chen, Dong-liang Wang, Zhi-qiang Ren, Chao Zeng, Zhao-lei Wang, De-shen Luo, Hui-yan Wang, Feng Qiu, Miao-zhen Bai, Long Zhang, Dong-sheng Wang, Feng-hua Li, Yu-hong Xu, Rui-hua |
author_sort | Chen, Dong-liang |
collection | PubMed |
description | BACKGROUND: Paxillin (PXN) has been found to be aberrantly regulated in various malignancies and involved in tumor growth and invasion. The clinicopathological and prognostic significance of PXN in gastric cancer is still unclear. METHODS: The expression of PXN was determined in paired gastric cancer tissues and adjacent normal tissues by Western blotting and real-time PCR. Immunohistochemistry was performed to detect the expression of PXN in 239 gastric cancer patients. Statistical analysis was applied to investigate the correlation between PXN expression and clinicopathological characteristics and prognosis in patients. Additionally, the effects of PXN on gastric cancer cell proliferation and migration were also evaluated. RESULTS: PXN was up-regulated in gastric cancer tissues and cell lines as compared with adjacent normal tissues and normal gastric epithelial cell line GES-1. Overexpression of PXN was correlated with distant metastasis (P = 0.001) and advanced tumor stage (P = 0.021) in gastric cancer patients. Patients with high PXN expression tended to have poor prognosis compared with patients with low PXN expression (P < 0.001). Multivariate analysis demonstrated that PXN expression was an independent prognostic factor (P = 0.020). Moreover, ectopic expression of PXN promotes cell proliferation and migration in AGS cells whereas knockdown of PXN inhibits cell proliferation and migration in SGC7901 cells. CONCLUSIONS: PXN plays an important role in tumor progression and may be used as a potential prognostic indicator in gastric cancer. |
format | Online Article Text |
id | pubmed-4228400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42284002014-11-13 Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer Chen, Dong-liang Wang, Zhi-qiang Ren, Chao Zeng, Zhao-lei Wang, De-shen Luo, Hui-yan Wang, Feng Qiu, Miao-zhen Bai, Long Zhang, Dong-sheng Wang, Feng-hua Li, Yu-hong Xu, Rui-hua J Transl Med Research BACKGROUND: Paxillin (PXN) has been found to be aberrantly regulated in various malignancies and involved in tumor growth and invasion. The clinicopathological and prognostic significance of PXN in gastric cancer is still unclear. METHODS: The expression of PXN was determined in paired gastric cancer tissues and adjacent normal tissues by Western blotting and real-time PCR. Immunohistochemistry was performed to detect the expression of PXN in 239 gastric cancer patients. Statistical analysis was applied to investigate the correlation between PXN expression and clinicopathological characteristics and prognosis in patients. Additionally, the effects of PXN on gastric cancer cell proliferation and migration were also evaluated. RESULTS: PXN was up-regulated in gastric cancer tissues and cell lines as compared with adjacent normal tissues and normal gastric epithelial cell line GES-1. Overexpression of PXN was correlated with distant metastasis (P = 0.001) and advanced tumor stage (P = 0.021) in gastric cancer patients. Patients with high PXN expression tended to have poor prognosis compared with patients with low PXN expression (P < 0.001). Multivariate analysis demonstrated that PXN expression was an independent prognostic factor (P = 0.020). Moreover, ectopic expression of PXN promotes cell proliferation and migration in AGS cells whereas knockdown of PXN inhibits cell proliferation and migration in SGC7901 cells. CONCLUSIONS: PXN plays an important role in tumor progression and may be used as a potential prognostic indicator in gastric cancer. BioMed Central 2013-11-02 /pmc/articles/PMC4228400/ /pubmed/24180516 http://dx.doi.org/10.1186/1479-5876-11-277 Text en Copyright © 2013 Chen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Chen, Dong-liang Wang, Zhi-qiang Ren, Chao Zeng, Zhao-lei Wang, De-shen Luo, Hui-yan Wang, Feng Qiu, Miao-zhen Bai, Long Zhang, Dong-sheng Wang, Feng-hua Li, Yu-hong Xu, Rui-hua Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer |
title | Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer |
title_full | Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer |
title_fullStr | Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer |
title_full_unstemmed | Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer |
title_short | Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer |
title_sort | abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228400/ https://www.ncbi.nlm.nih.gov/pubmed/24180516 http://dx.doi.org/10.1186/1479-5876-11-277 |
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