Cargando…
Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria
BACKGROUND: The rapid diagnostic test (RDT) has been adopted in contemporary malaria control and management programmes around the world as it represents a fast and apt alternative for malaria diagnosis in a resource-limited setting. This study assessed the performance of a HRP-2/pLDH based RDT (Para...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228469/ https://www.ncbi.nlm.nih.gov/pubmed/24172045 http://dx.doi.org/10.1186/1475-2875-12-378 |
_version_ | 1782343990836723712 |
---|---|
author | Elahi, Rubayet Mohon, Abu Naser Khan, Wasif A Haque, Rashidul Alam, Mohammad Shafiul |
author_facet | Elahi, Rubayet Mohon, Abu Naser Khan, Wasif A Haque, Rashidul Alam, Mohammad Shafiul |
author_sort | Elahi, Rubayet |
collection | PubMed |
description | BACKGROUND: The rapid diagnostic test (RDT) has been adopted in contemporary malaria control and management programmes around the world as it represents a fast and apt alternative for malaria diagnosis in a resource-limited setting. This study assessed the performance of a HRP-2/pLDH based RDT (Parascreen® Pan/Pf) in a laboratory setting utilizing clinical samples obtained from the field. METHODS: Whole blood samples were obtained from febrile patients referred for malaria diagnosis by clinicians from two different Upazila Health Complexes (UHCs) located near the Bangladesh-India and Bangladesh-Myanmar border where malaria is endemic. RDT was performed on archived samples and sensitivity and specificity evaluated with expert microscopy (EM) and quantitative PCR (qPCR). RESULTS: A total of 327 clinical samples were made available for the study, of which 153 were Plasmodium falciparum-positive and 54 were Plasmodium vivax-positive. In comparison with EM, for P. falciparum malaria, the RDT had sensitivity: 96.0% (95% CI, 91.2-98.3) and specificity: 98.2% (95% CI, 94.6-99.5) and for P. vivax, sensitivity: 90.7% (95% CI, 78.9-96.5) and specificity: 98.9% (95% CI, 96.5-99.7). Comparison with qPCR showed, for P. falciparum malaria, sensitivity: 95.4% (95% CI, 90.5-98.0) and specificity: 98.8% (95% CI, 95.4-99.7) and for P. vivax malaria, sensitivity: 89.0% (95% CI,77.0-95.4) and specificity: 98.8% (95% CI, 96.5-99.7). Sensitivity varied according to different parasitaemia for falciparum and vivax malaria diagnosis. CONCLUSION: Parascreen® Pan/Pf Rapid test for malaria showed acceptable sensitivity and specificity in border belt endemic areas of Bangladesh when compared with EM and qPCR. |
format | Online Article Text |
id | pubmed-4228469 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42284692014-11-13 Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria Elahi, Rubayet Mohon, Abu Naser Khan, Wasif A Haque, Rashidul Alam, Mohammad Shafiul Malar J Research BACKGROUND: The rapid diagnostic test (RDT) has been adopted in contemporary malaria control and management programmes around the world as it represents a fast and apt alternative for malaria diagnosis in a resource-limited setting. This study assessed the performance of a HRP-2/pLDH based RDT (Parascreen® Pan/Pf) in a laboratory setting utilizing clinical samples obtained from the field. METHODS: Whole blood samples were obtained from febrile patients referred for malaria diagnosis by clinicians from two different Upazila Health Complexes (UHCs) located near the Bangladesh-India and Bangladesh-Myanmar border where malaria is endemic. RDT was performed on archived samples and sensitivity and specificity evaluated with expert microscopy (EM) and quantitative PCR (qPCR). RESULTS: A total of 327 clinical samples were made available for the study, of which 153 were Plasmodium falciparum-positive and 54 were Plasmodium vivax-positive. In comparison with EM, for P. falciparum malaria, the RDT had sensitivity: 96.0% (95% CI, 91.2-98.3) and specificity: 98.2% (95% CI, 94.6-99.5) and for P. vivax, sensitivity: 90.7% (95% CI, 78.9-96.5) and specificity: 98.9% (95% CI, 96.5-99.7). Comparison with qPCR showed, for P. falciparum malaria, sensitivity: 95.4% (95% CI, 90.5-98.0) and specificity: 98.8% (95% CI, 95.4-99.7) and for P. vivax malaria, sensitivity: 89.0% (95% CI,77.0-95.4) and specificity: 98.8% (95% CI, 96.5-99.7). Sensitivity varied according to different parasitaemia for falciparum and vivax malaria diagnosis. CONCLUSION: Parascreen® Pan/Pf Rapid test for malaria showed acceptable sensitivity and specificity in border belt endemic areas of Bangladesh when compared with EM and qPCR. BioMed Central 2013-10-30 /pmc/articles/PMC4228469/ /pubmed/24172045 http://dx.doi.org/10.1186/1475-2875-12-378 Text en Copyright © 2013 Elahi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Elahi, Rubayet Mohon, Abu Naser Khan, Wasif A Haque, Rashidul Alam, Mohammad Shafiul Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria |
title | Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria |
title_full | Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria |
title_fullStr | Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria |
title_full_unstemmed | Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria |
title_short | Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria |
title_sort | performance of a hrp-2/pldh based rapid diagnostic test at the bangladesh-india-myanmar border areas for diagnosis of clinical malaria |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228469/ https://www.ncbi.nlm.nih.gov/pubmed/24172045 http://dx.doi.org/10.1186/1475-2875-12-378 |
work_keys_str_mv | AT elahirubayet performanceofahrp2pldhbasedrapiddiagnostictestatthebangladeshindiamyanmarborderareasfordiagnosisofclinicalmalaria AT mohonabunaser performanceofahrp2pldhbasedrapiddiagnostictestatthebangladeshindiamyanmarborderareasfordiagnosisofclinicalmalaria AT khanwasifa performanceofahrp2pldhbasedrapiddiagnostictestatthebangladeshindiamyanmarborderareasfordiagnosisofclinicalmalaria AT haquerashidul performanceofahrp2pldhbasedrapiddiagnostictestatthebangladeshindiamyanmarborderareasfordiagnosisofclinicalmalaria AT alammohammadshafiul performanceofahrp2pldhbasedrapiddiagnostictestatthebangladeshindiamyanmarborderareasfordiagnosisofclinicalmalaria |