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Isolation, Characterization, Crystal Structure Elucidation, and Anticancer Study of Dimethyl Cardamonin, Isolated from Syzygium campanulatum Korth
Syzygium campanulatum Korth is an equatorial, evergreen, aboriginal shrub of Malaysia. Conventionally it has been used as a stomachic. However, in the currently conducted study dimethyl cardamonin or 2′,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC) was isolated from S. campanulatum Korth, lea...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228816/ https://www.ncbi.nlm.nih.gov/pubmed/25530783 http://dx.doi.org/10.1155/2014/470179 |
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author | Memon, Abdul Hakeem Ismail, Zhari Aisha, Abdalrahim F. A. Al-Suede, Fouad Saleih Resq Hamil, Mohammad Shahrul Ridzuan Hashim, Suzana Saeed, Mohammed Ali Ahmed Laghari, Madeeha Abdul Majid, Amin Malik Shah |
author_facet | Memon, Abdul Hakeem Ismail, Zhari Aisha, Abdalrahim F. A. Al-Suede, Fouad Saleih Resq Hamil, Mohammad Shahrul Ridzuan Hashim, Suzana Saeed, Mohammed Ali Ahmed Laghari, Madeeha Abdul Majid, Amin Malik Shah |
author_sort | Memon, Abdul Hakeem |
collection | PubMed |
description | Syzygium campanulatum Korth is an equatorial, evergreen, aboriginal shrub of Malaysia. Conventionally it has been used as a stomachic. However, in the currently conducted study dimethyl cardamonin or 2′,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC) was isolated from S. campanulatum Korth, leaf extract. The structural characterization of DMC was carried out by making use of various techniques including UV, IR, NMR spectral followed by LC-MS, and X-ray crystallographic techniques. For determining the purity of compound, highly effective techniques including TLC, HPLC, and melting point were used. The cytotoxicity of DMC and three different extracts of S. campanulatum was evaluated against human colon cancer cell line (HT-29) by three different assays. DMC and ethanolic extract revealed potent and dose-dependent cytotoxic activity on the cancer cell line with IC(50) 12.6 and 90.1 µg/mL, respectively. Quite astonishingly to our knowledge, this is the very first report on S. campanulatum as being a rich source (3.5%) of DMC, X-ray crystallography, and anticancer activity on human colon cancer cells. |
format | Online Article Text |
id | pubmed-4228816 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-42288162014-12-21 Isolation, Characterization, Crystal Structure Elucidation, and Anticancer Study of Dimethyl Cardamonin, Isolated from Syzygium campanulatum Korth Memon, Abdul Hakeem Ismail, Zhari Aisha, Abdalrahim F. A. Al-Suede, Fouad Saleih Resq Hamil, Mohammad Shahrul Ridzuan Hashim, Suzana Saeed, Mohammed Ali Ahmed Laghari, Madeeha Abdul Majid, Amin Malik Shah Evid Based Complement Alternat Med Research Article Syzygium campanulatum Korth is an equatorial, evergreen, aboriginal shrub of Malaysia. Conventionally it has been used as a stomachic. However, in the currently conducted study dimethyl cardamonin or 2′,4′-dihydroxy-6′-methoxy-3′,5′-dimethylchalcone (DMC) was isolated from S. campanulatum Korth, leaf extract. The structural characterization of DMC was carried out by making use of various techniques including UV, IR, NMR spectral followed by LC-MS, and X-ray crystallographic techniques. For determining the purity of compound, highly effective techniques including TLC, HPLC, and melting point were used. The cytotoxicity of DMC and three different extracts of S. campanulatum was evaluated against human colon cancer cell line (HT-29) by three different assays. DMC and ethanolic extract revealed potent and dose-dependent cytotoxic activity on the cancer cell line with IC(50) 12.6 and 90.1 µg/mL, respectively. Quite astonishingly to our knowledge, this is the very first report on S. campanulatum as being a rich source (3.5%) of DMC, X-ray crystallography, and anticancer activity on human colon cancer cells. Hindawi Publishing Corporation 2014 2014-10-28 /pmc/articles/PMC4228816/ /pubmed/25530783 http://dx.doi.org/10.1155/2014/470179 Text en Copyright © 2014 Abdul Hakeem Memon et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Memon, Abdul Hakeem Ismail, Zhari Aisha, Abdalrahim F. A. Al-Suede, Fouad Saleih Resq Hamil, Mohammad Shahrul Ridzuan Hashim, Suzana Saeed, Mohammed Ali Ahmed Laghari, Madeeha Abdul Majid, Amin Malik Shah Isolation, Characterization, Crystal Structure Elucidation, and Anticancer Study of Dimethyl Cardamonin, Isolated from Syzygium campanulatum Korth |
title | Isolation, Characterization, Crystal Structure Elucidation, and Anticancer Study of Dimethyl Cardamonin, Isolated from Syzygium campanulatum Korth |
title_full | Isolation, Characterization, Crystal Structure Elucidation, and Anticancer Study of Dimethyl Cardamonin, Isolated from Syzygium campanulatum Korth |
title_fullStr | Isolation, Characterization, Crystal Structure Elucidation, and Anticancer Study of Dimethyl Cardamonin, Isolated from Syzygium campanulatum Korth |
title_full_unstemmed | Isolation, Characterization, Crystal Structure Elucidation, and Anticancer Study of Dimethyl Cardamonin, Isolated from Syzygium campanulatum Korth |
title_short | Isolation, Characterization, Crystal Structure Elucidation, and Anticancer Study of Dimethyl Cardamonin, Isolated from Syzygium campanulatum Korth |
title_sort | isolation, characterization, crystal structure elucidation, and anticancer study of dimethyl cardamonin, isolated from syzygium campanulatum korth |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4228816/ https://www.ncbi.nlm.nih.gov/pubmed/25530783 http://dx.doi.org/10.1155/2014/470179 |
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