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Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy

Active cell migration and invasion is a peculiar feature of glioma that makes this tumor able to rapidly infiltrate into the surrounding brain tissue. In our recent work, we identified a novel class of glioma-associated-stem cells (defined as GASC for high-grade glioma -HG- and Gasc for low-grade gl...

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Autores principales: Andolfi, Laura, Bourkoula, Eugenia, Migliorini, Elisa, Palma, Anita, Pucer, Anja, Skrap, Miran, Scoles, Giacinto, Beltrami, Antonio Paolo, Cesselli, Daniela, Lazzarino, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4229222/
https://www.ncbi.nlm.nih.gov/pubmed/25390644
http://dx.doi.org/10.1371/journal.pone.0112582
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author Andolfi, Laura
Bourkoula, Eugenia
Migliorini, Elisa
Palma, Anita
Pucer, Anja
Skrap, Miran
Scoles, Giacinto
Beltrami, Antonio Paolo
Cesselli, Daniela
Lazzarino, Marco
author_facet Andolfi, Laura
Bourkoula, Eugenia
Migliorini, Elisa
Palma, Anita
Pucer, Anja
Skrap, Miran
Scoles, Giacinto
Beltrami, Antonio Paolo
Cesselli, Daniela
Lazzarino, Marco
author_sort Andolfi, Laura
collection PubMed
description Active cell migration and invasion is a peculiar feature of glioma that makes this tumor able to rapidly infiltrate into the surrounding brain tissue. In our recent work, we identified a novel class of glioma-associated-stem cells (defined as GASC for high-grade glioma -HG- and Gasc for low-grade glioma -LG-) that, although not tumorigenic, act supporting the biological aggressiveness of glioma-initiating stem cells (defined as GSC for HG and Gsc for LG) favoring also their motility. Migrating cancer cells undergo considerable molecular and cellular changes by remodeling their cytoskeleton and cell interactions with surrounding environment. To get a better understanding about the role of the glioma-associated-stem cells in tumor progression, cell deformability and interactions between glioma-initiating stem cells and glioma-associated-stem cells were investigated. Adhesion of HG/LG-cancer cells on HG/LG-glioma-associated stem cells was studied by time-lapse microscopy, while cell deformability and cell-cell adhesion strengths were quantified by indentation measurements by atomic force microscopy and single cell force spectroscopy. Our results demonstrate that for both HG and LG glioma, cancer-initiating-stem cells are softer than glioma-associated-stem cells, in agreement with their neoplastic features. The adhesion strength of GSC on GASC appears to be significantly lower than that observed for Gsc on Gasc. Whereas, GSC spread and firmly adhere on Gasc with an adhesion strength increased as compared to that obtained on GASC. These findings highlight that the grade of glioma-associated-stem cells plays an important role in modulating cancer cell adhesion, which could affect glioma cell migration, invasion and thus cancer aggressiveness. Moreover this work provides evidence about the importance of investigating cell adhesion and elasticity for new developments in disease diagnostics and therapeutics.
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spelling pubmed-42292222014-11-18 Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy Andolfi, Laura Bourkoula, Eugenia Migliorini, Elisa Palma, Anita Pucer, Anja Skrap, Miran Scoles, Giacinto Beltrami, Antonio Paolo Cesselli, Daniela Lazzarino, Marco PLoS One Research Article Active cell migration and invasion is a peculiar feature of glioma that makes this tumor able to rapidly infiltrate into the surrounding brain tissue. In our recent work, we identified a novel class of glioma-associated-stem cells (defined as GASC for high-grade glioma -HG- and Gasc for low-grade glioma -LG-) that, although not tumorigenic, act supporting the biological aggressiveness of glioma-initiating stem cells (defined as GSC for HG and Gsc for LG) favoring also their motility. Migrating cancer cells undergo considerable molecular and cellular changes by remodeling their cytoskeleton and cell interactions with surrounding environment. To get a better understanding about the role of the glioma-associated-stem cells in tumor progression, cell deformability and interactions between glioma-initiating stem cells and glioma-associated-stem cells were investigated. Adhesion of HG/LG-cancer cells on HG/LG-glioma-associated stem cells was studied by time-lapse microscopy, while cell deformability and cell-cell adhesion strengths were quantified by indentation measurements by atomic force microscopy and single cell force spectroscopy. Our results demonstrate that for both HG and LG glioma, cancer-initiating-stem cells are softer than glioma-associated-stem cells, in agreement with their neoplastic features. The adhesion strength of GSC on GASC appears to be significantly lower than that observed for Gsc on Gasc. Whereas, GSC spread and firmly adhere on Gasc with an adhesion strength increased as compared to that obtained on GASC. These findings highlight that the grade of glioma-associated-stem cells plays an important role in modulating cancer cell adhesion, which could affect glioma cell migration, invasion and thus cancer aggressiveness. Moreover this work provides evidence about the importance of investigating cell adhesion and elasticity for new developments in disease diagnostics and therapeutics. Public Library of Science 2014-11-12 /pmc/articles/PMC4229222/ /pubmed/25390644 http://dx.doi.org/10.1371/journal.pone.0112582 Text en © 2014 Andolfi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Andolfi, Laura
Bourkoula, Eugenia
Migliorini, Elisa
Palma, Anita
Pucer, Anja
Skrap, Miran
Scoles, Giacinto
Beltrami, Antonio Paolo
Cesselli, Daniela
Lazzarino, Marco
Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy
title Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy
title_full Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy
title_fullStr Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy
title_full_unstemmed Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy
title_short Investigation of Adhesion and Mechanical Properties of Human Glioma Cells by Single Cell Force Spectroscopy and Atomic Force Microscopy
title_sort investigation of adhesion and mechanical properties of human glioma cells by single cell force spectroscopy and atomic force microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4229222/
https://www.ncbi.nlm.nih.gov/pubmed/25390644
http://dx.doi.org/10.1371/journal.pone.0112582
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