Customized patterned substrates for highly versatile correlative light-scanning electron microscopy

Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical...

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Detalles Bibliográficos
Autores principales: Benedetti, Lorena, Sogne, Elisa, Rodighiero, Simona, Marchesi, Davide, Milani, Paolo, Francolini, Maura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4229662/
https://www.ncbi.nlm.nih.gov/pubmed/25391455
http://dx.doi.org/10.1038/srep07033
Descripción
Sumario:Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.

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